Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel method for inducing plant chromosome variation by injecting zebularine in booting stage

A technology of chromosomes, new methods, applied in the field of agricultural biology

Active Publication Date: 2016-06-29
NANJING AGRICULTURAL UNIVERSITY
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chromosomal variants obtained in the above studies are chimeras that occur in the root apical cells. It has not been reported whether these variants can occur in the shoot apical meristem and other species, and thus whether they can be stably transmitted to the offspring.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel method for inducing plant chromosome variation by injecting zebularine in booting stage
  • Novel method for inducing plant chromosome variation by injecting zebularine in booting stage
  • Novel method for inducing plant chromosome variation by injecting zebularine in booting stage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] The present invention will be further described below in conjunction with the examples, and the experimental methods that do not specify specific conditions in the following examples generally follow the known methods in the art.

[0019] 1. The mutagenesis method of Chromosome 1 mutation in Jinghui

[0020] Wheat-rye diploid Jinghui 1 was used as the material, and the material was planted in the field. Young ears with a length of 3 to 4 cm at the booting stage were selected, and 100 μL of concentration was injected with a 1 mL syringe at 0.5 to 1 cm above the first internode under the ear. It is 500μM zebularine, and the single ear after injection is harvested as a single ear after maturity, and the harvested seeds are subjected to cytological identification to detect the type and frequency of chromosomal variation.

[0021] Solution preparation: 25mgzebularine was dissolved in 1562.5μL DMSO, then 3887.5μLddH was added 2 O, made into a mother solution with a concentra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of agricultural biology, and provides a novel method for inducing plant chromosome number and structure variation through a DNA transmethylase inhibitor zebularine.According to the method, octaploid jinghui-No.1 triticale is taken as a test material, zebularine is directly injected into plants in a booting stage, selfed seeds are harvested and subjected to chromosome identification, the chromosome number and structure variation which can be stably transferred is found from chromosome identification, and a novel method is supplied to plant chromosome engineering breeding and relevant theoretical research thereof.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology and relates to a new method for inducing plant chromosome variation. Background technique [0002] With artificial selection and intensive cultivation, the genetic base of many cultivated plants is gradually narrowing. It is an important way to broaden the genetic diversity of cultivated plants by inducing plant chromosome variation and transferring and utilizing favorable genes of related species through distant hybridization and chromosome engineering technology. How to efficiently induce chromosomal variation is one of the main research contents of crop chromosome engineering. [0003] In wheat (Triticum aestivumL, 2n=6x=42) chromosome engineering, commonly used chromosomal mutation mutagenesis methods include ionizing radiation, gametocidal chromosomes, wheat ph1b gene mutants and spontaneous variation (JiangJM, FriebeB, GillBS.Recentadvancesinalienegenetransferinwheat.Euphytica, 199...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 庄丽芳马旭辉亓增军王艳芝王青马洁云刘朋吴楠
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com