Multi-color in-situ hybridization method for rapidly analyzing and detecting triticeae plant chromosome configuration

A technique for rapid analysis and in situ hybridization, which is applied in the fields of biochemical equipment and methods, microbial determination/inspection, etc.

Inactive Publication Date: 2017-12-19
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the one-time identification of exchange recombination between different genomes of the Triticum family during meiosis has not been reported

Method used

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  • Multi-color in-situ hybridization method for rapidly analyzing and detecting triticeae plant chromosome configuration
  • Multi-color in-situ hybridization method for rapidly analyzing and detecting triticeae plant chromosome configuration
  • Multi-color in-situ hybridization method for rapidly analyzing and detecting triticeae plant chromosome configuration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0056] Example 2. Identification of octoploid wheat XY7430 material

[0057] 1. Preparation of octoploid wheat XY7430 pollen mother cell meiotic metaphase I mitotic phase

[0058] The octoploid wheat XY7430 is from the germplasm resources preserved by Professor Wang Honggang, College of Agriculture, Shandong Agricultural University.

[0059] 1) Selection of anthers at metaphase meiosis of pollen mother cell I

[0060] When the flag leaf of the plant is drawn out 2-3cm, select a spikelet with a size of about 7cm, select the anthers in different florets (3 anthers in each floret), stain with modified Carbo Fuchsin, and select the pollen mother cells in the microscopic examination. The anthers of metaphase meiosis I, and the remaining anthers in the same floret are used for preservation.

[0061] 2) Anther fixation and preservation

[0062] Place the anthers selected in step 1) in the French fixative (glacial acetic acid: absolute ethanol volume ratio = 1:3) for 12-24 hours, wash twice wit...

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Abstract

The invention relates to the technical field of biology, and especially relates to a multicolor in-situ hybridization method for rapidly analyzing and detecting triticeae plant chromosome configuration. The method is characterized in that mc-GISH is used for analysis of a chromosome pairing behavior during a pollen blast cell meiosis process, one-time hybridization can effectively identify the chromosome pairing condition during a meiosis process of triticeae at different genomes, and the method provides a powerful tool for analyzing a genetic relationship between the different genomes of triticeae, and especially for identifying the synapsis and pairing behaviors between different genomes of artificial created polyploid species.

Description

Technical field [0001] The invention relates to the field of biotechnology, mainly to the research fields of cell biology and molecular biology, and in particular to a multicolor in situ hybridization method for rapidly analyzing and detecting the chromosome configuration of Triticeae plants. Background technique [0002] Polyploid (polyploid) refers to a living individual that contains all the chromosomes of one or more ancestors attached in a cell, and is a form of life ubiquitous in plants. Due to the different formation methods, polyploid is now divided into autopolyploid and allopolyploid (Comai 2005; Wolfe 2001). Some important economic crops, such as wheat (Triticum aestivum), oats (Avena sativa), potato (Solanumtuberosum), cotton (Gossypium hirsutum), etc. are all polyploid; other crops, such as cabbage (Brassicaoleracea), corn (Zea mays ), soybean (Glycine max), sunflower (Helianthus annuus), etc. are diploids, but their ancestors have also undergone a process of polypl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6841C12Q1/6895C12Q2563/107
Inventor 何方刘树兵李兴锋鲍印广王洪刚
Owner SHANDONG AGRICULTURAL UNIVERSITY
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