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Single chromosome producing method through micro cutting plant chromosomes

A single chromosome and chromosome technology, applied in the field of plant chromosome acquisition, can solve the problems of high equipment requirements, high cost, and inconvenient promotion

Inactive Publication Date: 2012-02-15
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method is simple to operate, easy to master, and more accurate, it requires relatively high equipment conditions, high cost, and is not easy to promote.

Method used

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  • Single chromosome producing method through micro cutting plant chromosomes
  • Single chromosome producing method through micro cutting plant chromosomes
  • Single chromosome producing method through micro cutting plant chromosomes

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] The operation steps of the present invention will be described below by taking the wheat seed variety "Chinese Spring" as an example.

[0038] The first step is to obtain the seed root

[0039] Soak the Chinese spring seeds in sterile water at 25°C for 24 hours, then transfer them to a refrigerator at 4°C for 24 hours, and then culture them in the dark at 25°C. When the root grows to about 1cm, take it out, and use scissors to cut off the tip of the root at about 0.5cm , pretreated in ice water for 24 hours, then transferred to 70% ethanol and stored at 4°C until use;

[0040] The second step, the acquisition of pollen mother cells (hereinafter referred to as anthers)

[0041]Under field conditions, from May 1st to May 10th, Chinese spring wheat pollen was taken (observed under a microscope, in meiotic metaphase I), fixed with a solution of ethanol: glacial acetic acid = 3: 1 volume ratio for 10 minutes, and immediately Transfer to 70% ethanol and store at 4°C for lat...

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Abstract

The invention discloses a single chromosome producing method through micro cutting plant chromosomes, which comprises the steps that: under a microscope, the cool laser is adopted to cut and separate the seed plant chromosomes or chromatid segments of a specific section which is required to be researched according to the user requirement, and accordingly, any one chromatid or chromatid segment can be quickly and accurately obtained. The method adopts cool laser cutting, the laser is not directly contacted with the separated sample, the chromosome sample cannot be damaged due to the heat effect, and the problems that serious pollution is caused, the separated target chromosome is difficult to completely recovery, the operation equipment is complicated and the like in the former chromosome cutting technology are solved.

Description

technical field [0001] The invention relates to a method for obtaining plant chromosomes. Background technique [0002] 1. In the nuclei of cells that have not yet undergone division, many deep and slender meshes that can be stained can be seen, which is chromatin. When a cell divides, the chromatin in the nucleus presents a certain number and shape of chromosomes (chromosome), and the genes that control various biological traits are arranged in a straight line on the chromosome in a certain order (such as figure 1 ). [0003] 2. Chromosomes are the most important components in the nucleus. Chromosomes can be seen under an optical microscope or an electron microscope, and the chromosomes of various species of animals and plants in nature have specific morphological characteristics. During cell division, there are a series of regular changes in the morphology and structure of chromosomes. Among them, the metaphase and early anaphase of mitosis are the most obvious and typi...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12Q1/68
Inventor 毕建杰高居荣谭秀山尹赟瑜刘连颖盛一璟
Owner SHANDONG AGRICULTURAL UNIVERSITY
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