Chinese cabbage fertility relevant gene and method of using the gene in constituting artificial male sterility line of Chinese cabbage and similar crops

A male sterile line and genetic technology, applied in genetic engineering, botany equipment and methods, biochemical equipment and methods, etc., can solve crop self-incompatibility instability, difficulty in parental reproduction, time-consuming Power and other issues

Inactive Publication Date: 2003-11-05
ZHEJIANG UNIV
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Problems solved by technology

However, the method of artificial detasseling is time-consuming and labor-intensive, and the cost of seed production is high; chemical detasseling provides a new method for hybrid seed production, but it has disadvantages such as poor detasseling effect, inhibiting plant growth, and polluting the environment; Cross-incompatibility is easily affected by the environment and unstable, and t

Method used

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  • Chinese cabbage fertility relevant gene and method of using the gene in constituting artificial male sterility line of Chinese cabbage and similar crops
  • Chinese cabbage fertility relevant gene and method of using the gene in constituting artificial male sterility line of Chinese cabbage and similar crops

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Experimental program
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Embodiment Construction

[0016] Take the construction of 'Shanghai Qing' common cabbage and 'Youqing' cabbage as an example.

[0017] Obtaining Antisense Fragments of Fertility Related Genes

[0018] According to the full-length sequence (SEQ ID No.1) of the Chinese cabbage fertility-related gene, primers were designed between the 901st base and the 1338th base in the gene, and the 438bp sequence was amplified as an antisense gene fragment (SEQ ID No.2). And according to the multiple cloning restriction site of plant binary vector pBI121 ( figure 1 ), respectively design two PCR amplification primers containing BamH I and Xba I restriction endonuclease sites and three protective bases, the sequence of which is: P1 5'-GCT GGA TCC ACC ATT AGG TTT TTT AGA CA- 3'; P2 5'-GATTCT AGA GCC ACT AAC AAA CTT CCC TG-3'. The PCR amplification conditions were: 94°C, pre-denaturation for 3min; 94°C, denaturation for 1min; 50°C, annealing for 45s; 72°C, extension for 45s; 72°C, extension for 10min; storage at 4°C; ...

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Abstract

By means of plant gene engineering, primer is designed from No. 901 base to No. 1338 base inside the fertility relevant gene, 438 bp sequence is amplified as anti-sense gene segment and introduced into plant chromosome to obtain regenerative plant and artificial male sterile common Chinese cabbage and core. The said method is suitable for rape genus plants and may be used in fast constituting the male sterility line of different Chinese cabbage and similar vegetable crops without the limitation similar to that of seeking natural sterile source. The present invention opens one new way the wide application of plant gene engineering technology in agricultural production.

Description

technical field [0001] The invention relates to a Chinese cabbage fertility-related gene and a method for using the gene to construct an artificial male sterile line of Chinese cabbage crops. Background technique [0002] The utilization of heterosis is of great significance in crop breeding aimed at improving the yield and quality of crops, and enhancing the disease resistance and stress resistance of varieties. At present, the methods of using heterosis to formulate and produce the first generation of hybrids in crop production mainly include artificial detasseling, chemical detasseling, using self-incompatible lines and male sterile lines. However, the method of artificial detasseling is time-consuming and labor-intensive, and the cost of seed production is high; chemical detasseling provides a new method for hybrid seed production, but it has disadvantages such as poor detasseling effect, inhibiting plant growth, and polluting the environment; ...

Claims

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Application Information

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IPC IPC(8): A01H1/00A01H4/00A01H5/00C12N15/29C12N15/70C12N15/74C12Q1/68
Inventor 曹家树余小林叶纨芝
Owner ZHEJIANG UNIV
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