71 results about "Spermoblast" patented technology

The invention relates to the technical field of biomedical detection, and discloses a non-invasive sperm cell death and viability detection method, which utilizes a bright field image and a fluorescence image to train a fluorescence-assisted bright field attention network, introduces a fluorescence attention guidance module to provide guidance for training of a Transformer encoder, and realizes non-invasive sperm cell death and viability detection through priori knowledge of the fluorescence image. By means of the method, the model can be quickly focused on key features displayed in the fluorescent highlight area, the situation that training resources are consumed on a large amount of irrelevant information is avoided, convergence is accelerated, the model training time is shortened, meanwhile, the detection precision is improved, and then a more accurate and efficient sperm cell death and activity detection model is obtained. And during actual detection, the sperm cell death and viability detection can be realized only by collecting a microscopic image, pre-processing the microscopic image and inputting the preprocessed microscopic image into the model without dyeing a sperm sample, so that the damage of a traditional detection method to sperms is avoided, the non-invasive detection of the sperm cell death and viability is realized, and the method is more suitable for clinical detection.

The invention provides application of a protein deacetylase inhibitor NAM in ultralow-temperature cryopreservation of poultry semen, and belongs to the technical field of poultry breed conservation. The protein deacetylase inhibitor NAM is used as a cryoprotectant or is combined with other cryoprotectants for use, and the protein deacetylase inhibitor NAM is used as an effective component, so that the ultralow-temperature cryopreservation efficiency of poultry can be improved. Wherein the protein deacetylase inhibitor NAM can inhibit sperm cell deacetylation modification so as to maintain a high acetylation modification level, improve the ability of sperms to respond to ultralow temperature freezing stimulation and improve the fertilization ability of frozen-remelted sperms.

The invention relates to a sperm cell analysis and diagnosis system based on a multi-modal large language model, which comprises a multi-modal data co-processing unit, a cross-modal semantic alignment module, a dynamic diagnosis decision engine and a self-adaptive evolution system, the four-dimensional data processing module is used for synchronously processing microscopic images, motion trail videos, biochemical detection data and four-dimensional input data of medical record texts and comprises a feature selector based on a gating attention mechanism. According to the sperm cell analysis and diagnosis system based on the multi-modal large language model, quantitative analysis of sperm movement chaos features is realized for the first time, a nonlinear dynamic evaluation standard is established, a cross-modal knowledge distillation and meta-learning migration framework is developed, a data annotation dependence bottleneck is broken through, and an interpretable clinical decision support system is constructed; dynamic updating and probabilistic suggestion of diagnosis rules are achieved, semantic analysis of single-cell multi-omics data is achieved, and molecular mechanism research results are converted into clinically available knowledge.

The present invention provides a method for accurately measuring live sperm morphology, including the following steps: S11. acquiring an image including a plurality of sperm cells; S12. detecting the sperm cells in the image, and segmenting a head, a midpiece, and a tail of each detected sperm cell; and S13. calculating morphological parameters of each sperm cell based on a segmentation result. For the method for accurately measuring live sperm morphology of the present invention, an image of sperm cells is acquired, a head, a midpiece, and a tail of each sperm cell are segmented through image processing, and morphological parameters of each sperm cell are calculated based on a segmentation result. Morphological parameters of live sperm cells can be comprehensively and accurately obtained for analysis or selection.

Means for rigorous quantitative assessment of sperm motility are presented, based on a number of movement and morphology parameters measured using various image processing methods. The quantitative assessment thereby achieved is objective and multidimensional, allowing for detailed and repeatable assessment of samples. Deep learning methods are also disclosed for detection and classification of sperm cells (or other types of cells or particles) using neural networks, adapted to work both in good and in poor imaging condition, such as low magnification and resolution. Specific methods for both supervised and unsupervised deep learning approaches are delineated. In a non-limiting disclosure, the methods are particularly adapted to deal with cases of azoospermia, where there is a very low number of sperm cells (most of which do not swim) and a lot of debris in the imaged field of view.

The present invention relates to a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation. According to a first aspect of the present invention, there is provided a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation, the method comprising:providing an image of the sperm cell, under brightfield and / or phase contrast with a total magnification of 400× to 1000×;evaluating the image of the sperm cell to identify and / or measure a pre-selected biomarker; andapproximating the output of the pre-selected chemical assay of sperm DNA fragmentation of the sperm cell by subjecting the identified and / or measured biomarker to a first machine learning analysis.

The present invention relates to a composition comprising an elderberry extract as an effective component for preventing, treating, or alleviating andropause syndrome, wherein the elderberry extract of the present invention increases testosterone secretion in sperm cells and andropause syndrome animal models, and thus can be usefully employed as a pharmaceutical or food composition for preventing, treating or alleviating andropause syndrome.

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

A discrimination system with optics that collect output electromagnetic radiation from sperm cells within a measurement region, a split detector that generates an electrical signal responsive to the intensity of the collected electromagnetic radiation and which detects the position of sperm cells in the measurement region, and an analyzer for discriminating between X-chromosome bearing sperm and Y-chromosome bearing sperm in the measurement region.

The invention discloses a micro-fluidic chip for exploring in-vitro XY type sperm fertilization preference, and relates to the technical field of biomedical engineering.According to the technical scheme, the micro-fluidic chip is characterized by comprising a chip body, the chip body is provided with a central sample carrying hole, the central sample carrying hole is used for containing an oocyte, and the central sample carrying hole is communicated with the central sample carrying hole; the chip body is provided with four peripheral sample carrying holes, the four peripheral sample carrying holes are used for placing fluorescently-labeled sperm cells subjected to sexual control sorting, and communicated micro-channels are arranged between the central sample carrying hole and the four peripheral sample carrying holes. Observation is facilitated by improving the volume of a liquid carrying hole and optimizing the transparency of a material, meanwhile, by adjusting the diameter of a channel and the number of repeated units, the thickness of the micro-fluidic chip is reduced, micro-channel blockage is avoided, the number of cells in loaded liquid is guaranteed, and the detection accuracy of the micro-fluidic chip is improved. The preference of successful fertilization of X and Y type sperms under different conditions is observed in vitro through micro-fluidic, and a new thought is provided for a method for controlling the gender of a fetus.

The present invention provides a cryoprotective additive for human sperm and a preparation method thereof. The method is to add an mTORC1 activator and a Caspase-9 inhibitor to a commercially available sperm cryoprotectant. Compared with the pure commercially available protectant, the cryoprotective additive of the present invention has an increased number of forward-moving sperm after thawing and recovery; there are more forward-moving sperm after recovery, with better forward motility and better forward motility endurance. At the same time, the mitochondrial structure and function of the sperm cells after recovery are less damaged, the mitochondrial function recovers quickly, less mitochondrial ROS is produced, apoptosis is reduced, the ability to protect the function of sperm cells is better, and it is more conducive to the long-term cryopreservation of sperm-like cells.

Provided herein are methods, reagents, and kits for the isolation of sperm cells from other cell types and the purification of sperm-associated DNA from a complex sample. In particular, methods, reagents, and kits are provided for the separation of intact sperm cells from epithelial cells via binding of sperm cells to a cellulose material, and the extraction of sperm-associated DNA therefrom.

The present disclosure relates to methods of identifying, differentiating between and / or sorting sperm cells, or more particularly, X-chromosome bearing sperm (X-CBS) and Y-chromosome bearing sperm (Y-CBS) cells, in a sperm sample, the methods comprising determining a level of a Reactive Oxidant Species and / or a Cellular Change mediated thereby (ROSCC) in each of a plurality of sperm cells in the sperm sample; and based on the determined ROSCC levels, identifying, differentiating between and / or sorting X-CBS and Y-CBS cells in at least a portion of the plurality of sperm cells. Also described are enriched or purified X-CBS and Y-CBS cells, and uses thereof.

The invention discloses an efficient extraction method of blue fox sperm DNA, and belongs to the technical field of molecular biology. Aiming at the structural characteristics that chromatin of the blue fox sperms is highly condensed, histone is replaced by protamine, and a stable nucleoprotein complex is formed through an intermolecular disulfide bond, the invention provides an efficient DNA extraction method suitable for the blue fox sperms. According to the method, based on the synergistic effect of three chemical reagents, namely SDS, PK and DTT, full lysis of the sperm cells of the blue foxes is achieved, and then efficient enrichment of sperm DNA of the blue foxes can be achieved by combining a conventional DNA extraction method. According to the efficient extraction method of the blue fox sperm DNA provided by the invention, the yield and integrity of the blue fox sperm DNA are remarkably improved, and the limitation that sperm chromatin is difficult to effectively lyse by a conventional method is overcome; the method provides reliable technical support for research on genetic diversity evaluation, population management, molecular breeding and the like of the blue foxes, and has the potential of popularization and application in other high-condensation sperm species.

The application discloses a high-precision sperm morphology screening system and a screening method, and relates to the technical field of sperm cell recognition. Raw semen is obtained and filtered to obtain initial semen; the initial semen is spread into a preset single-layer sperm liquid strip to obtain initial single-layer motile sperm; a first magnification operation is performed on the initial single-layer motile sperm to obtain a first initial single-layer motile sperm image; a second magnification operation is performed on the first initial single-layer motile sperm image to obtain a second initial single-layer motile sperm image; and the second initial single-layer motile sperm image is substituted into a high-precision sperm morphology selection model to obtain target high-quality sperm. Through the preliminary filtration of the raw semen and the secondary screening of the preset single-layer sperm strip spreading method, and by adopting the progressive magnification strategy of the first magnification operation and the second magnification operation, the high-precision sperm morphology screening can be performed when high-quality sperm is selected by using the existing IMSI technology, and the screening efficiency of the high-precision sperm is improved.

The present invention relates to a sperm cell selection device having a support plate comprising an inlet for receiving a sample of sperm cells, an outlet for collecting at least some of the sperm cells contained in the sample, and a microfluidic system located between the inlet and the outlet, wherein the microfluidic system comprises a tank for receiving a culture medium, and the tank has an inlet region and an outlet region in the vicinity of the inlet and the outlet, respectively, and wherein the inlet and the outlet are arranged such that when a culture medium is added into the tank through the outlet, the culture medium is introduced into the tank through the outlet. Providing a hydrostatic pressure difference between the inlet region and the outlet region to cause the medium to flow from the outlet region to the inlet region to initiate sperm cells in a sperm cell sample to migrate from the inlet region to the outlet region, and wherein the inlet region and the outlet region are in fluid communication with each other via a main channel arranged in the slot, the channel having a region of reduced width arranged between the inlet region and the outlet region to form a taxis region, whereby the sperm cell sample has undergone selection by passing through the taxis region during the sperm cell migration so that a premium sperm cell sample can be collected from the exit region at the exit.

The utility model discloses an efficient separator for human seminal spot mixed spots, which relates to the technical field of separators and comprises a centrifugal tube and a detachable sleeve matched with the centrifugal tube, the caliber of the detachable sleeve is consistent with that of the centrifugal tube, and a sleeve cavity for containing biological material evidences is arranged in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve cavity, a filter membrane filter layer is arranged at the bottom of the sleeve cavity, and a bottom drainage hole is formed in the bottom of the detachable sleeve. According to the utility model, a two-stage filtering mode of carrier removal filtering and sperm enrichment filtering is constructed in one sleeve, so that a biological material evidence carrier can be efficiently removed, water-soluble substances of human epithelial cells can be quickly filtered, sperm cells can be efficiently separated from related biological material evidences only by using a small amount of biological material evidences, and the operation is simple and convenient. The test speed and efficiency are greatly improved, difficult biological material evidence treatment is simplified, the experiment repeatability is good, the applicability is high, biological material evidence samples are saved, and the cost of related experiments is reduced.

The present invention discloses a method for detecting highly active sperm without chemical staining, comprising: collecting digital images of healthy sperm without chemical staining by using a sperm activity sorting and observation device based on microfluidics; performing non-linear intelligent color transfer processing on the collected digital images by using a complex deep neural network color transfer model; performing binary processing on the R and B channels of the stained images to respectively determine the head positions and tail positions of each sperm, jointly determining the positions of the corresponding complete sperm by the two and intercepting them; inputting the sperm images into a sperm discrimination convolutional neural network based on deep learning to discriminate whether they are complete and healthy. The present invention realizes chemical-staining-free imaging of sperm cells during the sorting process of the sperm activity sorting and observation device based on microfluidics, enabling cells to achieve the same observation and detection effects as those after staining without chemical staining, and improving the efficiency of sperm observation and detection.

A device for sperm cell selection has a support plate comprising an inlet to receive a sample of sperm cells, an outlet to collect at least some of the sperm cells comprised in the sample, and a microfluidic system therebetween. The microfluidic system comprises a basin receiving a medium and having inlet and outlet zones. The inlet and outlet provide a hydrostatic pressure difference between the zones when medium is added to the basin via the outlet, to provoke a stream of the medium from the outlet zone towards the inlet zone to initiate sperm cell migration from the inlet zone towards the outlet zone. The basin has a zone of reduced width between the zones, to form a rheotaxis zone. The sample of sperm cells undergoes a selection by passing through the rheotaxis zone during migration for collecting quality sperm cells from the outlet zone at the outlet.

This invention discloses a method, system, device, medium, and application for constructing a diagnostic model for male infertility, belonging to the fields of male infertility diagnosis, drug screening, and assisted reproductive technology. In this invention, by simultaneously and quantitatively analyzing the pH value, mitochondrial G-quadruplex structure level, and β-galactosidase activity within a single sperm cell, and integrating routine clinical sperm testing parameters and age, a high-precision diagnostic model for male infertility and a drug screening system are established, providing a reference for assisted reproduction to meet practical needs.

The present disclosure relates to methods and kits for treatment of biological samples comprising non-sperm cells and sperm cells. The present disclosure relates more particularly to methods and kits for treatment of forensic samples comprising non-sperm cells and sperm cells.

The present invention provides an antifreeze polypeptide derived from bovine collagen, which can reduce the difference in solute concentration inside and outside the cell during the cooling process, thereby inhibiting the formation of ice crystals inside and outside the sperm cell, and further reducing ice crystal damage to cell membranes and organelles during deep cryopreservation and recovery, thereby improving the survival rate of sperm cells after deep cryopreservation and recovery. The frozen sperm has a higher in vitro fertilization cell development rate and fertility rate, and can reproduce rapidly. In addition, the semen freezing diluent provided by the present application does not contain any exogenous protein, which can effectively reduce exogenous protein interference and immunosuppression during in vitro fertilization, and further improve the fertilization rate and fertility rate of frozen semen.

The invention relates to a protective liquid formula for improving the survival rate of frozen and thawed sperms of pigs. The protective liquid formula comprises the following components in percentage by mass: 14% of sodium hexadecylsulfonate, 7% of coconut oil monoethanolamide and 79% of distilled water. The preparation method comprises the following steps: 1) weighing 14g of sodium hexadecylsulfonate and 7g of coconut oil monoethanolamide, and dissolving in 79g of distilled water; and 2) uniformly stirring the solution obtained in the step 1) at 60 DEG C until the sodium hexadecyl sulfonate and the coconut oil monoethanolamide are completely dissolved in the distilled water. The protection liquid has the advantages that the protection liquid protects porcine sperm cells, provides a suitable living environment for porcine sperms, improves the oxidative stress resistance and the preservation effect of the porcine sperms, ensures the survival rate of the porcine sperms, and further improves the breeding rate and the breeding benefits of sows; the survival rate, the sperm motility and the acrosome integrity rate of the frozen and thawed sperms of the pigs are obviously improved; the feed additive is simple in proportioning, high in reliability, low in economic cost, high in biological value and convenient to popularize and apply in production, and yolk with extremely high preparation requirements is not used.

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

The invention consists of methods and compositions for detecting the presence or absence of a DNA aberration by analyzing fluorescence emission characteristics in sperm cells or sperm nuclei, which generally consists of entraining sperm cells or sperm nuclei stained with a DNA selective dye in sheath fluid; exposing the entrained sperm cells or sperm nuclei to electromagnetic radiation; determining a forward fluorescence characteristic and a side fluorescence characteristic of individual events associated with the exposed sperm cells or sperm nuclei; and gating the individual events based on the forward fluorescence characteristic and the side fluorescence characteristic with a criterion.

There is provided a system and method for detection of sperm using virtual-staining. The method including: receiving an unstained inference image, the inference image including a microscopic image capturing sperm cells; generating a virtual-stained image of sperm from the inference image using a trained generator machine learning model, the generator machine learning model taking the inference image as input, the generator machine learning model trained using a set of training images including microscopic images of sperm cells and a set of ground-truth images showing staining that identifies the sperm cells in the training images, the generator machine learning model trained by propagating determined losses between generated virtual-stained images and corresponding ground-truth images; and outputting the generated virtual-stained image of sperm.

The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population using flow-cytometric processing. The methods generally entail staining the sperm cells in the population; irradiating the sperm cells in the population; detecting fluorescence emitted by the sperm cells in the population in response to the step of irradiating; differentiating the sperm cells in the population based on orientation or viability; and then collecting a subpopulation of oriented or viable sperm cells.

Embodiments of this application provide a method, apparatus, device, and computer-readable storage device for sperm identification based on microscope images. The method includes acquiring a microscope image; segmenting the microscope image to obtain all cell instances; classifying the cell instances to obtain the shape and position features of each cell; and inputting the shape and position features of each cell into a trained decision tree classification model to identify normal sperm cells. In this way, normal sperm can be accurately and quickly located from a microscope image.