40 results about "Spermoblast" patented technology

The invention relates to a sperm cell analysis and diagnosis system based on a multi-modal large language model, which comprises a multi-modal data co-processing unit, a cross-modal semantic alignment module, a dynamic diagnosis decision engine and a self-adaptive evolution system, the four-dimensional data processing module is used for synchronously processing microscopic images, motion trail videos, biochemical detection data and four-dimensional input data of medical record texts and comprises a feature selector based on a gating attention mechanism. According to the sperm cell analysis and diagnosis system based on the multi-modal large language model, quantitative analysis of sperm movement chaos features is realized for the first time, a nonlinear dynamic evaluation standard is established, a cross-modal knowledge distillation and meta-learning migration framework is developed, a data annotation dependence bottleneck is broken through, and an interpretable clinical decision support system is constructed; dynamic updating and probabilistic suggestion of diagnosis rules are achieved, semantic analysis of single-cell multi-omics data is achieved, and molecular mechanism research results are converted into clinically available knowledge.

Means for rigorous quantitative assessment of sperm motility are presented, based on a number of movement and morphology parameters measured using various image processing methods. The quantitative assessment thereby achieved is objective and multidimensional, allowing for detailed and repeatable assessment of samples. Deep learning methods are also disclosed for detection and classification of sperm cells (or other types of cells or particles) using neural networks, adapted to work both in good and in poor imaging condition, such as low magnification and resolution. Specific methods for both supervised and unsupervised deep learning approaches are delineated. In a non-limiting disclosure, the methods are particularly adapted to deal with cases of azoospermia, where there is a very low number of sperm cells (most of which do not swim) and a lot of debris in the imaged field of view.

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

The present disclosure relates to methods of identifying, differentiating between and / or sorting sperm cells, or more particularly, X-chromosome bearing sperm (X-CBS) and Y-chromosome bearing sperm (Y-CBS) cells, in a sperm sample, the methods comprising determining a level of a Reactive Oxidant Species and / or a Cellular Change mediated thereby (ROSCC) in each of a plurality of sperm cells in the sperm sample; and based on the determined ROSCC levels, identifying, differentiating between and / or sorting X-CBS and Y-CBS cells in at least a portion of the plurality of sperm cells. Also described are enriched or purified X-CBS and Y-CBS cells, and uses thereof.

The invention discloses an efficient extraction method of blue fox sperm DNA, and belongs to the technical field of molecular biology. Aiming at the structural characteristics that chromatin of the blue fox sperms is highly condensed, histone is replaced by protamine, and a stable nucleoprotein complex is formed through an intermolecular disulfide bond, the invention provides an efficient DNA extraction method suitable for the blue fox sperms. According to the method, based on the synergistic effect of three chemical reagents, namely SDS, PK and DTT, full lysis of the sperm cells of the blue foxes is achieved, and then efficient enrichment of sperm DNA of the blue foxes can be achieved by combining a conventional DNA extraction method. According to the efficient extraction method of the blue fox sperm DNA provided by the invention, the yield and integrity of the blue fox sperm DNA are remarkably improved, and the limitation that sperm chromatin is difficult to effectively lyse by a conventional method is overcome; the method provides reliable technical support for research on genetic diversity evaluation, population management, molecular breeding and the like of the blue foxes, and has the potential of popularization and application in other high-condensation sperm species.

The application discloses a high-precision sperm morphology screening system and a screening method, and relates to the technical field of sperm cell recognition. Raw semen is obtained and filtered to obtain initial semen; the initial semen is spread into a preset single-layer sperm liquid strip to obtain initial single-layer motile sperm; a first magnification operation is performed on the initial single-layer motile sperm to obtain a first initial single-layer motile sperm image; a second magnification operation is performed on the first initial single-layer motile sperm image to obtain a second initial single-layer motile sperm image; and the second initial single-layer motile sperm image is substituted into a high-precision sperm morphology selection model to obtain target high-quality sperm. Through the preliminary filtration of the raw semen and the secondary screening of the preset single-layer sperm strip spreading method, and by adopting the progressive magnification strategy of the first magnification operation and the second magnification operation, the high-precision sperm morphology screening can be performed when high-quality sperm is selected by using the existing IMSI technology, and the screening efficiency of the high-precision sperm is improved.

The utility model discloses an efficient separator for human seminal spot mixed spots, which relates to the technical field of separators and comprises a centrifugal tube and a detachable sleeve matched with the centrifugal tube, the caliber of the detachable sleeve is consistent with that of the centrifugal tube, and a sleeve cavity for containing biological material evidences is arranged in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve cavity, a filter membrane filter layer is arranged at the bottom of the sleeve cavity, and a bottom drainage hole is formed in the bottom of the detachable sleeve. According to the utility model, a two-stage filtering mode of carrier removal filtering and sperm enrichment filtering is constructed in one sleeve, so that a biological material evidence carrier can be efficiently removed, water-soluble substances of human epithelial cells can be quickly filtered, sperm cells can be efficiently separated from related biological material evidences only by using a small amount of biological material evidences, and the operation is simple and convenient. The test speed and efficiency are greatly improved, difficult biological material evidence treatment is simplified, the experiment repeatability is good, the applicability is high, biological material evidence samples are saved, and the cost of related experiments is reduced.

A device for sperm cell selection has a support plate comprising an inlet to receive a sample of sperm cells, an outlet to collect at least some of the sperm cells comprised in the sample, and a microfluidic system therebetween. The microfluidic system comprises a basin receiving a medium and having inlet and outlet zones. The inlet and outlet provide a hydrostatic pressure difference between the zones when medium is added to the basin via the outlet, to provoke a stream of the medium from the outlet zone towards the inlet zone to initiate sperm cell migration from the inlet zone towards the outlet zone. The basin has a zone of reduced width between the zones, to form a rheotaxis zone. The sample of sperm cells undergoes a selection by passing through the rheotaxis zone during migration for collecting quality sperm cells from the outlet zone at the outlet.

This invention discloses a method, system, device, medium, and application for constructing a diagnostic model for male infertility, belonging to the fields of male infertility diagnosis, drug screening, and assisted reproductive technology. In this invention, by simultaneously and quantitatively analyzing the pH value, mitochondrial G-quadruplex structure level, and β-galactosidase activity within a single sperm cell, and integrating routine clinical sperm testing parameters and age, a high-precision diagnostic model for male infertility and a drug screening system are established, providing a reference for assisted reproduction to meet practical needs.

The present disclosure relates to methods and kits for treatment of biological samples comprising non-sperm cells and sperm cells. The present disclosure relates more particularly to methods and kits for treatment of forensic samples comprising non-sperm cells and sperm cells.

The invention relates to a protective liquid formula for improving the survival rate of frozen and thawed sperms of pigs. The protective liquid formula comprises the following components in percentage by mass: 14% of sodium hexadecylsulfonate, 7% of coconut oil monoethanolamide and 79% of distilled water. The preparation method comprises the following steps: 1) weighing 14g of sodium hexadecylsulfonate and 7g of coconut oil monoethanolamide, and dissolving in 79g of distilled water; and 2) uniformly stirring the solution obtained in the step 1) at 60 DEG C until the sodium hexadecyl sulfonate and the coconut oil monoethanolamide are completely dissolved in the distilled water. The protection liquid has the advantages that the protection liquid protects porcine sperm cells, provides a suitable living environment for porcine sperms, improves the oxidative stress resistance and the preservation effect of the porcine sperms, ensures the survival rate of the porcine sperms, and further improves the breeding rate and the breeding benefits of sows; the survival rate, the sperm motility and the acrosome integrity rate of the frozen and thawed sperms of the pigs are obviously improved; the feed additive is simple in proportioning, high in reliability, low in economic cost, high in biological value and convenient to popularize and apply in production, and yolk with extremely high preparation requirements is not used.

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

The invention consists of methods and compositions for detecting the presence or absence of a DNA aberration by analyzing fluorescence emission characteristics in sperm cells or sperm nuclei, which generally consists of entraining sperm cells or sperm nuclei stained with a DNA selective dye in sheath fluid; exposing the entrained sperm cells or sperm nuclei to electromagnetic radiation; determining a forward fluorescence characteristic and a side fluorescence characteristic of individual events associated with the exposed sperm cells or sperm nuclei; and gating the individual events based on the forward fluorescence characteristic and the side fluorescence characteristic with a criterion.

The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population using flow-cytometric processing. The methods generally entail staining the sperm cells in the population; irradiating the sperm cells in the population; detecting fluorescence emitted by the sperm cells in the population in response to the step of irradiating; differentiating the sperm cells in the population based on orientation or viability; and then collecting a subpopulation of oriented or viable sperm cells.

Embodiments of this application provide a method, apparatus, device, and computer-readable storage device for sperm identification based on microscope images. The method includes acquiring a microscope image; segmenting the microscope image to obtain all cell instances; classifying the cell instances to obtain the shape and position features of each cell; and inputting the shape and position features of each cell into a trained decision tree classification model to identify normal sperm cells. In this way, normal sperm can be accurately and quickly located from a microscope image.

The application provides a hidden sperm screening system and method based on magnetic bead capture and fluorescent identification, and is applied to the technical field of magnetic bead fluorescence analysis. A sperm sample is incubated with high-magnetic-saturation first immunomagnetic beads and low-magnetic-saturation second immunomagnetic beads in an incubation module, and the two kinds of magnetic beads are coupled with anti-sperm antibodies and anti-non-sperm cell antibodies respectively. A magnetic field generating module applies an alternating magnetic field to a separation area and synchronously injects sheath liquid, and uses the difference in magnetic response characteristics to retain the complex combined with sperm and to flush away the complex combined with non-sperm cells. A dye introduction module introduces membrane impermeable and permeable dyes under the constraint of a magnetic field, and obtains fluorescent characteristics of membrane-intact and membrane-damaged sperm which can be distinguished. An imaging module performs multi-channel fluorescence imaging, an image analysis module extracts positive events conforming to morphology and fluorescent logic, and finally outputs sperm count parameters and membrane integrity classification parameters synchronously.

The present invention relates to a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation. According to a first aspect of the present invention, there is provided a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation, the method comprising:providing an image of the sperm cell, under brightfield and / or phase contrast with a total magnification of 400× to 1000×;evaluating the image of the sperm cell to identify and / or measure a pre-selected biomarker; andapproximating the output of the pre-selected chemical assay of sperm DNA fragmentation of the sperm cell by subjecting the identified and / or measured biomarker to a first machine learning analysis.

A composition includes a contraceptive chimeric virus-like particle with an antigenic carrier domain and one or more antigenic regions from a sperm cell in the antigenic carrier domain, with the antigenic carrier domain including human papillomavirus L1 capsid protein and the antigenic regions including one or more structural elements of the Catsper ion channel complex. When administered to a patient, the contraceptive vaccine stimulates production of anti-sperm antibodies that, upon binding to a sperm cell, inhibit the sperm cell's motility and thus inhibit the ability of the sperm cell to fertilize an egg cell. The induced immunoinfertility of the composition can be reversed for brief or extended lengths of time by overdosing the patient with a reversal agent lacking the antigenic carrier domain but having a protein sequence substantially identical to that of the one or more antigenic regions to sequester the anti-sperm antibodies.

The present invention relates to a sperm cell selection device having a support plate comprising an inlet for receiving a sperm cell sample, an outlet for collecting at least a portion of the sperm cells comprised in said sample, and a microfluidic system between said inlet and outlet, wherein the microfluidic system comprises a cuvette arranged to receive a medium and having an inlet area and an outlet area respectively proximate the inlet and the outlet, and wherein the inlet and the outlet are arranged to provide a hydrostatic pressure difference between said inlet area and said outlet area when a medium is added to the cuvette via the outlet,so as to cause a flow of said medium from the outlet area toward the inlet area to initiate sperm cell migration of the sperm cells of the sperm cell sample from the inlet area toward the outlet area, and wherein the inlet area and the outlet area are in fluid communication with each other via a main channel arranged in the cuvette, the channel having a reduced width area arranged between the inlet area and the outlet area so as to form a rheotaxis zone, whereby said sperm cell sample undergoes selection by passing through said rheotaxis zone during said sperm cell migration thereby enabling a quality sperm cell sample to be collected from the outlet area at the outlet. Figure for abstract: Fig. 1,

This invention provides a sperm cell counting detection plate, including a main frame. Auxiliary blocks are fixed to the four corners of the outer side of the upper surface of the main frame. A cover plate is rotatably connected to the top rear end of the main frame via a hinge. A coverslip is fixed to the middle of the cover plate, and fixing blocks are fixed to the four corners of the bottom outer side of the cover plate. A glass slide is fixed to the middle of the main frame, and a main storage cylinder is fixed to the middle of the glass slide. Secondary storage cylinders are fixed to the left and right ends of the middle of the glass slide. An inner frame is fixed to the outer side of the inner frame, and a return spring and a contact plate are embedded inside the inner frame. This invention can achieve multiple sealing protection for the semen in the main storage cylinder, effectively preventing sperm leakage caused by device shaking after semen is added, making subsequent observation and counting more convenient.

The application provides application of a protein deacetylase inhibitor NAM in poultry semen ultra-low temperature cryopreservation and belongs to the technical field of poultry breeding preservation. The protein deacetylase inhibitor NAM is used as a cryoprotective agent or is combined with other cryoprotective agents, and the protein deacetylase inhibitor NAM is used as an effective component, so that the efficiency of the poultry ultra-low temperature cryopreservation can be improved. The protein deacetylase inhibitor NAM can inhibit sperm cell deacetylation modification, maintain a high level of acetylation modification level, improve the ability of sperm to respond to ultra-low temperature freezing stimulation, and improve the fertilization ability of frozen-thawed sperm.

The invention discloses a sperm cell detection-based processing method and a storage medium, a microscope camera is used to shoot sperm to be detected at fixed time intervals, and an improved YOLO target detection model is used to carry out sperm cell detection, so that accurate detection of sperm cells is realized; according to the method, sperm cells are tracked by using a sperm cell tracking model, sperm cell information is recorded into a database, data support is provided for subsequent analysis, and a method for judging the motion trail and length of target sperm cells is established according to the sperm cell information database. According to the method, the number of the sperm frame images is set, the motion trail length corresponding to the number of the sperm frame images and the sperm cell activity degree judgment threshold value are set, the sperm activity degree can be objectively evaluated, a scientific basis is provided for sperm quality evaluation, the sperm quality can be efficiently and accurately evaluated, and important data support is provided for reproductive medicine research.

The present invention relates to a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation. According to a first aspect of the present invention, there is provided a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation, the method comprising:providing an image of the sperm cell, under brightfield and / or phase contrast with a total magnification of 400× to 1000×;evaluating the image of the sperm cell to identify and / or measure a pre-selected biomarker; andapproximating the output of the pre-selected chemical assay of sperm DNA fragmentation of the sperm cell by subjecting the identified and / or measured biomarker to a first machine learning analysis.

The invention discloses a method and a system for evaluating male fertility by analyzing meiosis recombination rate. The method comprises the following steps: acquiring a diploid biological sample and a plurality of monomer sperm cells of a subject; respectively determining the diploid and haploid genotypes on the heterozygous loci through sequencing; identifying and filtering meiosis crossover events for each sperm cell based on the genotype data, thereby calculating a quantitative crossover rate; the crossover rate is finally compared to a reference crossover rate from a normal fertility population to assess the fertility of the subject. The key point of the invention lies in that it is revealed for the first time that reduction of meiosis crossover rate is a potential quantitative biomarker of'uniform cell energy deficiency 'in male germ cell lines, and the method can more deeply, objectively and accurately evaluate the internal health status of sperms by measuring the core development event. The kit is especially suitable for diagnosis and risk assessment of asthenospermia and monitoring of infertility treatment effect.

The invention relates to a flow cytometry installation, in particular for the processing of spermatozoon-type cells and in particular for the sexing of animal semen, comprising an optical detection system (3) configured to differentiate, according to predetermined parameters, a plurality of cells (26) in a jet of such cells (11) directed towards a differentiation zone (17), and an imaging system (40) configured to visualize the jet comprising the differentiated cells at the level of the differentiation zone and to determine representative information of a time at which a drop containing an identified cell should be generated, and then sorted;with the optical detection system (3) which is provided with a first light radiation source (12) configured to emit a light beam (16) focused on the differentiation zone (17), at least two detection elements (14) configured to detect each a quantity of light re-emitted by the cells (26) which are illuminated in different directions of re-emission, and at least two collection elements (15) which are focused, on a first side, on the differentiation zone (17) and on a second side opposite to the first side, on the respective detection elements (14);the installation being characterized in that at least one of the two collection organs (15) is formed by a lens (60, 70) having a cut edge (61, 71) and in that the imaging system (40) is provided with a camera (41) and a second light source (42), distinct from the first light source (12), which is arranged and configured so as to generate a viewing ray (43) directed towards the differentiation zone (17) and the camera. Figure for the abbreviation: Figure 3;

The invention provides a yak sperm cryopreservation liquid and a preparation method thereof, modified cholesterol is added into the cryopreservation liquid, and the modified cholesterol is obtained by grafting alginic acid with cholesterol and then grafting glutathione. After the modified cholesterol is added into the preserving fluid, a three-dimensional cross-linked network is formed by means of glycerol glycidyl ether, and the network can reduce the size of ice crystals in the freezing process and form a barrier layer outside a sperm cell membrane, so that the ice crystals are prevented from directly damaging the cell membrane and acrosome. Meanwhile, the compatibility of the cholesterol and the sperm cell membrane is excellent, and the modified cholesterol can be enriched on the surface of the cell membrane, so that the effects of filling the membrane gap, enhancing the mechanical strength of the cell membrane and reducing the generation of ice crystals are achieved; meanwhile, glutathione can remove active oxygen, and cell membrane structures and sperm internal components are prevented from being damaged by oxidative stress; in addition, the modified cholesterol can also have a synergistic effect with egg yolk lipoprotein and bovine serum albumin, so that the vitality of frozen sperms is improved, the acrosome integrity rate is improved, and the malformation rate is reduced.

The invention relates to an IVF oligoasthenospermia deformity available sperm grading evaluation system based on an expansion hydrogel technology. The IVF oligoasthenospermia deformity available sperm grading evaluation system comprises an expansion hydrogel preparation unit, a sperm sample processing unit, a sperm grading evaluation unit and a data processing and analyzing unit, wherein the expansion hydrogel is prepared from diacetone acrylamide, N (hydroxymethyl) acrylamide and sodium acrylate. According to the method, accurate grading evaluation of the oligospermia and asthenospermia abnormal sperms is realized by utilizing the amplification effect of the expansion hydrogel on the sperm cell structure and combining a modern detection technology, and the IVF success rate and the embryo quality are improved.

The invention discloses a paracobitis variegata semen preserving fluid as well as a preparation method and application thereof, and belongs to the technical field of fish semen preservation. The preserving fluid comprises double distilled water, methanol, sodium chloride, potassium chloride, cane sugar, trehalose, yolk and bovine serum albumin. According to the method, spermatoblast metabolism of the paracobitis erythrocauda is inhibited by mixing the sperm of the paracobitis erythrocauda with the preservation solution and freezing the mixed solution of the sperm of the paracobitis erythrocauda and the preservation solution, and yolk and methanol in the preservation solution can improve the anti-freezing capacity of the spermatoblast of the paracobitis erythrocauda. Sodium chloride and potassium chloride in the preservation solution can simulate a body fluid osmotic pressure environment and protect spermatoblasts of the paracobitis erythrocauda, cane sugar and trehalose in the preservation solution provide energy for sperms of the paracobitis erythrocauda and ensure that the spermatoblasts of the paracobitis erythrocauda maintain vitality, and all components in the preservation solution have a mutual synergistic effect and protect the spermatoblasts of the paracobitis erythrocauda. The aims of preserving the semen of the paracobitis resurrectocauda for a long time and maintaining the sperm cell activity of the paracobitis