70 results about "Spermoblast" patented technology

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

The invention discloses a method for predicting whether sperms exist in the testis of an azoospermia patient or not according to seminal plasma mRNA. The method comprises the following steps of dividing seminal fluid specimens of the azoospermia patient into a testicular sperm group and a testicular azoospermia group by taking a testicular biopsy as a gold standard, determining the difference of exRNA expression profiles of two seminal fluid groups, carrying out comparison with an existing spermatogenic cell transcriptome database, and searching for differentially expressed exRNA capable of judging spermatogenic cell types, which is used for judging whether haploid sperm cells exist in the testis of the azoospermia patient or not. The clinical application of the scheme provided by the invention can greatly improve the sperm taking success rate of the azoospermia patient, and is also helpful for guiding the azoospermia patient to select an economical, painless and effective treatment method to solve the problem of infertility of the azoospermia patient.

The embodiment of the invention discloses a sperm fragmentization rate detection method, device and method and a readable storage medium, and the method comprises the steps: obtaining and preprocessing of an archeocyte image; obtaining all kernel image blocks, all first halo image blocks, all cell images and the corresponding relation between the image blocks and the cell images according to the archeocyte image after preprocessing; carrying out the analysis of the kernel image blocks, the corresponding first halo image blocks and the cell images; determining the sperm cells and the sperm kernel image blocks and sperm halo image blocks corresponding to the sperm cells according to an analysis result and the corresponding relation between the image blocks and the cell images; carrying out the analysis of the sperm kernel image blocks and the sperm halo image blocks corresponding to all sperm cells, and judging whether the corresponding sperm cell is qualified or not according to the analysis result; and carrying out the counting of the qualified sperm cells and the unqualified sperm cells, so as to obtain the sperm fragmentization rate. The method improves the work efficiency and the precision of the detection result.

Staining mixtures comprising viable spermatozoa, a composition which regulates oxidation/reduction reactions intracellularly or extracellularly, and a DNA selective dye are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for staining sperm cells comprising the formation of a staining mixture are also disclosed.

A sperm tail recognition method comprises the following steps: step 1, acquiring an image; step 2, acquiring a difference image; step 3, acquiring a tail image; 4, acquiring a sperm head region image;5, combining the tail image and the sperm head region image; and 6, filtering the bright spot area without the long-tail area within a preset distance to obtain a sperm area image. Through acquisition of the difference image, a bright spot area in the semen sample image is filtered out, so that a non-long-tail area is convenient to filter, and an image of impurities or cells, which are greatly different from the sperm structure, in semen is realized. Filtering of bright spot images without long-tail-shaped areas nearby can be achieved through a distance selection method, and then filtering ofbright spot areas of non-sperm cells is achieved. According to the method, impurities in the seminal fluid are accurately filtered, and researchers can count and research the seminal fluid conveniently.

A method for providing semen optimized for use in artificial insemination is described. Methods involve monitoring sperm cell metabolism by assays that produce results in real time, while sperm are still being processed into doses for use in insemination. Processing is modified in response to assay results, to optimize sperm performance.

The present disclosure is directed to methods for typing and characterizing sperm. The technology employs a sequencing-based method for detecting and measuring RNA transcripts from single sperm cells and the analysis of the sequencing data for the prediction of male parent contribution to autism.

The present invention relates to an immunological method that selects spermatozoa having X-chromosome or Y-chromosome. The method of the invention is based on the use of monoclonal antibodies directed against the genus-specific proteins located in the cytoplasm membrane of spermatozoa associated to the action of the classical complement pathway to increase the percentage of one of the gender in the offspring of mammals.

Provided are methods of in vitro maturation of human spermatogonium, comprising culturing the spermatogonium in a three-dimensional methylcellose culture system (MCS) under conditions capable of differentiating said human spermatogonium into an elongated spermatid, thereby in vitro maturing the human spermatogonium. Also provided is an in vitro matured sperm obtainable according to the method of the invention and methods of treating subjects in need of mature sperm cells.

The invention discloses an application of CLRN3 protein as cell surface identification protein for separating X sperms. The CLRN3 protein is in specific expression on the surface of the mammal X spermoblast, and an amino acid sequence of the CLRN3 protein is as shown in sequence 2 in a sequence table. The method for separating the X sperms provided by the invention is not only simple and easy to do, and the whole separation process is free from using dye and radiation, the pressure on the spermoblast is small, and the harmful influence on the sperm is relatively small. Through the method provided by the invention, the heavy demand on the sexed sperm in the production can be satisfied. The application disclosed by the invention has important application value.

The invention belongs to the technical field of forensic medicine, and particularly relates to a method for recognizing mixed semen stains from two individuals. According to the method, the individuals are separated and recognized by ABH antigen corresponding to ABO blood types of the individuals on spermoblast surfaces. The method particularly includes the steps: 1 preparing the mixed semen stains of the two individuals with ABH antigenic diversity; 2 performing immunohistochemical staining by the aid of labeled A, B and H antibodies, and collecting spermoblast of different ABH blood types bya cell capture technology; 3 performing PCR (polymerase chain reaction) amplification and capillary electrophoresis to perform genetic typing on STR (short tandem repeat) loci. According to the method for recognizing the mixed semen stains from the two individuals, individual origins of DNA (deoxyribonucleic acid) in the mixed semen stains of a plurality of individuals can be effectively determined according to gene level, the step of identifying histologic origins of body fluids or spots on criminal investigation scenes is greatly simplified, and accurate scientific evidence can be providedfor defining case quality, determining criminal suspects, performing conviction and sentencing and the like.

Microfluidic devices and methods for focusing components in a fluid sample are described herein. The microfluidic devices feature a microfluidic chip having a micro-channel having a constricting portion that narrows in width, and a flow focusing region downstream of the micro-channel. The flow focusing region includes a positively sloping bottom surface that reduces a height of the flow focusing region and sidewalls that taper to reduce a width of the flow focusing region, thereby geometrically constricting the flow focusing region. The devices and methods can be utilized in sex-sorting of sperm cells to improve performance and increase eligibility.

The invention discloses a dilution device. The dilution device comprises a mixing chamber, a first storage chamber and a second storage chamber are arranged above the mixing chamber, the first storagechamber is communicated with the mixing chamber through a first through flow hole, a puncture rod is arranged in the first storage chamber, a rotatable liquid mixer is arranged in the mixing chamber,and liquid distribution holes are arranged at the lower side of the liquid mixer. When the dilution device performs dilution operation, semen to be diluted is firstly added into the mixing chamber, adilution solution is added into the mixing chamber through the liquid mixer, the semen and the dilution solution are mixed, the arrangement of the liquid mixer makes the liquid mixer driven by a first drive to rotate during the addition of the dilution solution into the mixing chamber through the liquid mixer, and centrifugal force generated by the rotation is used to make the dilution solution uniformly flow into the mixing chamber through the liquid distribution holes in order to achieve no inactivation of spermoblasts during mixing. The invention relates to the field of mixing devices.

The invention belongs to the technical field of sperm morphology detection, and particularly relates to an automatic sperm morphology analysis method based on multi-scale feature fusion, which comprises the following steps: scanning a sperm sample image through a microscope automatic scanning platform, performing normalization processing on the obtained sperm sample image, extracting features of sperms on multiple scales by using an SD Net network model, fusing the features, outputting coordinates and bounding box information of the sperms on the sperm sample image, and detecting sperm images existing in the sperm sample image; and carrying out feature extraction and regional feature fusion on the detected sperm image by using an SR Net network model, and finally completing the recognition of abnormal sperm cells. Compared with a general deep learning method, the invention has the advantages that by fusing the features on multiple scales, the method has better detection capability on sperms with smaller sizes, the detection accuracy is higher, the generalization is stronger, and a very good detection effect can also be obtained under the condition of larger sample image difference.