16 results about "Spermoblast" patented technology

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

The present disclosure relates to methods of identifying, differentiating between and / or sorting sperm cells, or more particularly, X-chromosome bearing sperm (X-CBS) and Y-chromosome bearing sperm (Y-CBS) cells, in a sperm sample, the methods comprising determining a level of a Reactive Oxidant Species and / or a Cellular Change mediated thereby (ROSCC) in each of a plurality of sperm cells in the sperm sample; and based on the determined ROSCC levels, identifying, differentiating between and / or sorting X-CBS and Y-CBS cells in at least a portion of the plurality of sperm cells. Also described are enriched or purified X-CBS and Y-CBS cells, and uses thereof.

This invention discloses a method, system, device, medium, and application for constructing a diagnostic model for male infertility, belonging to the fields of male infertility diagnosis, drug screening, and assisted reproductive technology. In this invention, by simultaneously and quantitatively analyzing the pH value, mitochondrial G-quadruplex structure level, and β-galactosidase activity within a single sperm cell, and integrating routine clinical sperm testing parameters and age, a high-precision diagnostic model for male infertility and a drug screening system are established, providing a reference for assisted reproduction to meet practical needs.

The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population using flow-cytometric processing. The methods generally entail staining the sperm cells in the population; irradiating the sperm cells in the population; detecting fluorescence emitted by the sperm cells in the population in response to the step of irradiating; differentiating the sperm cells in the population based on orientation or viability; and then collecting a subpopulation of oriented or viable sperm cells.

A composition includes a contraceptive chimeric virus-like particle with an antigenic carrier domain and one or more antigenic regions from a sperm cell in the antigenic carrier domain, with the antigenic carrier domain including human papillomavirus L1 capsid protein and the antigenic regions including one or more structural elements of the Catsper ion channel complex. When administered to a patient, the contraceptive vaccine stimulates production of anti-sperm antibodies that, upon binding to a sperm cell, inhibit the sperm cell's motility and thus inhibit the ability of the sperm cell to fertilize an egg cell. The induced immunoinfertility of the composition can be reversed for brief or extended lengths of time by overdosing the patient with a reversal agent lacking the antigenic carrier domain but having a protein sequence substantially identical to that of the one or more antigenic regions to sequester the anti-sperm antibodies.

This invention provides a sperm cell counting detection plate, including a main frame. Auxiliary blocks are fixed to the four corners of the outer side of the upper surface of the main frame. A cover plate is rotatably connected to the top rear end of the main frame via a hinge. A coverslip is fixed to the middle of the cover plate, and fixing blocks are fixed to the four corners of the bottom outer side of the cover plate. A glass slide is fixed to the middle of the main frame, and a main storage cylinder is fixed to the middle of the glass slide. Secondary storage cylinders are fixed to the left and right ends of the middle of the glass slide. An inner frame is fixed to the outer side of the inner frame, and a return spring and a contact plate are embedded inside the inner frame. This invention can achieve multiple sealing protection for the semen in the main storage cylinder, effectively preventing sperm leakage caused by device shaking after semen is added, making subsequent observation and counting more convenient.

The invention discloses a method and a system for evaluating male fertility by analyzing meiosis recombination rate. The method comprises the following steps: acquiring a diploid biological sample and a plurality of monomer sperm cells of a subject; respectively determining the diploid and haploid genotypes on the heterozygous loci through sequencing; identifying and filtering meiosis crossover events for each sperm cell based on the genotype data, thereby calculating a quantitative crossover rate; the crossover rate is finally compared to a reference crossover rate from a normal fertility population to assess the fertility of the subject. The key point of the invention lies in that it is revealed for the first time that reduction of meiosis crossover rate is a potential quantitative biomarker of'uniform cell energy deficiency 'in male germ cell lines, and the method can more deeply, objectively and accurately evaluate the internal health status of sperms by measuring the core development event. The kit is especially suitable for diagnosis and risk assessment of asthenospermia and monitoring of infertility treatment effect.

The invention relates to a flow cytometry installation, in particular for the processing of spermatozoon-type cells and in particular for the sexing of animal semen, comprising an optical detection system (3) configured to differentiate, according to predetermined parameters, a plurality of cells (26) in a jet of such cells (11) directed towards a differentiation zone (17), and an imaging system (40) configured to visualize the jet comprising the differentiated cells at the level of the differentiation zone and to determine representative information of a time at which a drop containing an identified cell should be generated, and then sorted;with the optical detection system (3) which is provided with a first light radiation source (12) configured to emit a light beam (16) focused on the differentiation zone (17), at least two detection elements (14) configured to detect each a quantity of light re-emitted by the cells (26) which are illuminated in different directions of re-emission, and at least two collection elements (15) which are focused, on a first side, on the differentiation zone (17) and on a second side opposite to the first side, on the respective detection elements (14);the installation being characterized in that at least one of the two collection organs (15) is formed by a lens (60, 70) having a cut edge (61, 71) and in that the imaging system (40) is provided with a camera (41) and a second light source (42), distinct from the first light source (12), which is arranged and configured so as to generate a viewing ray (43) directed towards the differentiation zone (17) and the camera. Figure for the abbreviation: Figure 3;

The invention provides a yak sperm cryopreservation liquid and a preparation method thereof, modified cholesterol is added into the cryopreservation liquid, and the modified cholesterol is obtained by grafting alginic acid with cholesterol and then grafting glutathione. After the modified cholesterol is added into the preserving fluid, a three-dimensional cross-linked network is formed by means of glycerol glycidyl ether, and the network can reduce the size of ice crystals in the freezing process and form a barrier layer outside a sperm cell membrane, so that the ice crystals are prevented from directly damaging the cell membrane and acrosome. Meanwhile, the compatibility of the cholesterol and the sperm cell membrane is excellent, and the modified cholesterol can be enriched on the surface of the cell membrane, so that the effects of filling the membrane gap, enhancing the mechanical strength of the cell membrane and reducing the generation of ice crystals are achieved; meanwhile, glutathione can remove active oxygen, and cell membrane structures and sperm internal components are prevented from being damaged by oxidative stress; in addition, the modified cholesterol can also have a synergistic effect with egg yolk lipoprotein and bovine serum albumin, so that the vitality of frozen sperms is improved, the acrosome integrity rate is improved, and the malformation rate is reduced.

The invention relates to a method, a system and a composition for delivering macromolecules to marine fish sperms based on exosomes, and belongs to the crossing field of biotechnology and reproductive engineering.According to the method, the exosomes displaying CD81 protein serve as a carrier, and the macromolecules are delivered to marine fish sperms through specific binding of the CD81 protein and a receptor ligand of CD9 protein on a fish sperm cell membrane. And delivering the biological macromolecules loaded in the exosome into the marine fish sperms. Targeting and tracing are integrated, the targeting can be enhanced through the potential interaction effect of CD81 and sperm surface protein CD9, and real-time tracing of the delivery process can be achieved through EGFP fluorescence.

The invention discloses a method and a system for diagnosing and typing male infertility based on sperm RNA (Ribonucleic Acid) characteristics. The core of the invention lies in a biological discovery that most RNA can be systematically degraded and removed in the sperm maturation process, so that the total RNA content of healthy and mature sperms is in negative correlation with the functional soundness of the healthy and mature sperms. The method comprises the following steps: (a) obtaining a semen sample of a subject; (b) separating and purifying sperm cells from the sample; (c) quantifying the purified sperms, and determining the total RNA content of each sperm cell so as to obtain a first value; and (d) comparing the first numerical value with a preset reference value, and if the first numerical value is obviously higher than the reference value, indicating that the subject has low fertility or infertility (such as asthenospermia). According to the invention, an objective and quantifiable molecular biomarker is provided by quantifying residual'molecular package 'in the sperm maturation process, and a new way is provided for accurate diagnosis, typing and treatment of male infertility.

The present invention relates to a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation. According to a first aspect of the present invention, there is provided a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation, the method comprising:providing an image of the sperm cell, under brightfield and / or phase contrast with a total magnification of 400× to 1000×;evaluating the image of the sperm cell to identify and / or measure a pre-selected biomarker; andapproximating the output of the pre-selected chemical assay of sperm DNA fragmentation of the sperm cell by subjecting the identified and / or measured biomarker to a first machine learning analysis.

The application discloses a RNAi interference fragment targeting a donkey Zfy gene, a carrier and a preparation method and application thereof, and relates to the technical field of animal molecular genetics, wherein the RNAi interference fragment comprises RNAi-3-F and RNAi-3-R, and the nucleotide sequences are shown in SEQ ID NO:1 and SEQ ID NO:2 respectively. The RNAi interference fragment provided by the application can target and silence Y chromosome sex determination related genes in male donkey Y spermatogenesis stage key reproductive cells, hinder and interfere with the normal development and physiological functions of Y sperm, regulate the key genes of Y sperm development through specific silencing, realize sex regulation before fertilization, greatly improve the proportion of female individuals of offspring donkeys, and the scheme does not damage target sperm cells and does not reduce sperm activity, and meanwhile, the scheme has the advantages of simple operation and low production cost.

The invention discloses a functional artificial egg capable of reducing the malformation rate of sperms of boars and a preparation method thereof, and every kilogram of egg liquid in the artificial egg comprises the following components in percentage by weight: 25-35% of protein component; 20-28% of a fatty acid component; 25-30% of phospholipid and 4-5% of cholesterol; the vitamin components comprise 500 to 1000 [mu] g / kg of vitamin A, 5 to 10 [mu] g / kg of vitamin D, 80 to 120 [mu] g / kg of vitamin E, 3 to 5 [mu] g / kg of vitamin B12 and 1500 to 2500 mg / kg of carnitine; the mineral element components comprise 650 to 750 mg / kg of phosphorus, 450 to 550 mg / kg of potassium, 800 to 1000 mg / kg of calcium, 40 to 60 mg / kg of magnesium, 18 to 20 mg / kg of iron, 6 to 12 mg / kg of zinc and 120 to 180 [mu] g / kg of selenium; and the balance of water. On the basis of nutritional physiology of spermatogenesis of boars, protein components, fatty acid components, vitamins (A, D, E, B12 and carnitine) related to oxidation resistance / energy metabolism and mineral elements (zinc and selenium) are accurately compounded; the components have a synergistic effect in the aspects of promoting the integrity of a sperm cell membrane structure, providing an energy metabolism substrate and enhancing an anti-oxidation defense system, so that the sperm malformation rate is remarkably reduced.