64 results about "Molecular spectroscopy" patented technology

The present invention relates, e.g., to a method for determining the size distribution of DNA molecules in a sample comprising cell-free nucleic acid, comprising labeling the DNA with a fluorescent dye in a stoichiometric manner, subjecting the DNA to molecular spectroscopy (e.g., cylindrical illumination confocal spectroscopy), analyzing suitable fluorescent burst parameters of the labeled DNA, and conducting single molecule DNA integrity analysis of the labeled DNA molecules in the sample. In one embodiment of the invention, the method is used as a diagnostic method for detecting cancer.

The present invention relates, e.g., to a method for determining the size distribution of DNA molecules in a sample comprising cell-free nucleic acid, comprising labeling the DNA with a fluorescent dye in a stoichiometric manner, subjecting the DNA to molecular spectroscopy (e.g., cylindrical illumination confocal spectroscopy), analyzing suitable fluorescent burst parameters of the labeled DNA, and conducting single molecule DNA integrity analysis of the labeled DNA molecules in the sample. In one embodiment of the invention, the method is used as a diagnostic method for detecting cancer.

Methods and apparatus in the field of single molecule sensing are described, e.g. for molecular analysis of analytes such as molecular analytes, e.g. nucleic acids, proteins, polypeptides, peptides, lipids and polysaccharides. Molecular spectroscopy on a molecule translocating through a solid-state nanopore is described. Optical spectroscopic signals are enhanced by plasmonic field-confinement and antenna effects and probed in transmission by plasmon-enabled transmission of light through an optical channel that overlaps with the physical channel.

Described herein is a spectroscopic system and method for measuring and monitoring the chemical composition and / or impurity content of a sample or sample stream using absorption light spectroscopy. Specifically, in certain embodiments, this invention relates to the use of sample pressure variation to alter the magnitude of the absorption spectrum (e.g., wavelength-dependent signal) received for the sample, thereby obviating the need for a reference or ‘zero’ sample. Rather than use a reference or ‘zero’ sample, embodiments described herein obtain a spectrum / signal from a sample-containing cell at both a first pressure and a second (different) pressure.

The invention provides a device for simultaneously reducing an intensity noise and a frequency noise of single frequency laser and a working method thereof. The device comprises a single mode semiconductor pump light source, a laser power stabilizing device, a wavelength division multiplexer, a single frequency fiber resonant cavity and a fiber optic isolator. According to the method, based on the fact that at the low-frequency stage, the intensity noise and the frequency noise of a single frequency fiber laser both come from a pump laser intensity noise, the low-frequency intensity noise of the pump laser is directly reduced in an amplitude modulation mode, and accordingly the effect of simultaneously reducing the two noises at the low-frequency stage is achieved. A single frequency fiber laser source with ultra-stable output power and frequency is obtained through the simple and practical method, and the method has wide application prospect and huge application value in the fields of ultrahigh precision and super-remote distance laser ranging, optical fiber sensing and atom and molecular spectroscopy.

The invention discloses a synchronous acquisition device and method of an atom and molecular spectrum. The method comprises the following steps: striking the surface of a to-be-detected sample repeatedly by focused energy of a laser device, stimulating the to-be-detected sample to be at a plasma state, and collecting an atom spectrum transmitted by the to-be-detected sample at the plasma state by an optical fiber collector via a visible spectrograph; striking the surface of the to-be-detected sample repeatedly by the focused energy of the laser device, and collecting an infrared spectrum transmitted externally by the surface of the to-be-detected sample by an infrared optical fiber collector via the visible spectrograph so as to obtain a molecule spectrum; transmitting the molecular spectrum to a signal collecting control system by the atom spectrum and the infrared spectrograph via the visible spectrograph; and matching the atom spectrum and the molecular spectrum by the signal collecting control system so as to obtain the corresponding relationship between the atom spectral line and a functional group. The method can be used for obtaining the molecule and atom spectrum with the same size synchronously within a short time, and achieving the correlation of the characteristic relation of the atom spectrum and the molecular spectrum.

The invention discloses a broadband single-frequency fiber laser intensity noise suppression device for achieving a nearly schottky noise limit. The broadband single-frequency fiber laser intensity noise suppression device comprises a single-frequency fiber laser resonator, a wavelength division multiplexer, a single-mode semiconductor pump light source, an optical fiber isolator, a polarization controller, a laser saturated amplification device, an optical fiber filter module, an optical fiber coupler, a photoelectric detector and a feedback control system. According to the broadband single-frequency fiber laser intensity noise suppression device disclosed by the invention, the broadband intensity noise of output laser can be reduced through the laser saturated amplification device; feedback control is carried out on the laser saturated amplification device, so that the intensity noise of the output laser can be further reduced; the frequency range of intensity noise suppression can be further expanded; and finally, the effects of keeping the intensity noise of a single-frequency laser within a broadband and simultaneously reducing the intensity noise close to schottky noise limit are achieved. The broadband single-frequency fiber laser intensity noise suppression device for achieving the nearly schottky noise limit has a broad application prospect and great application value in the fields of ultra-high precision and ultra-long distance laser ranging, optical fiber sensing, atomic and molecular spectroscopy and the like.

The invention discloses a molecular spectrum imaging device applied in clinical medicine, biology, hylology, microelectronics and other subject fields. The invention is characterized in that: light sources are connected to dispersion units through optical fibers; the dispersion units are connected to a dark field optical collector through optical fibers and used for illumination of a microscope; a signal of an image sensor CCD is connected to a computer through a cable; a control wire of the computer is connected to a dispersion unit controller which is connected to the dispersion units; the computer changes the wavelength of the light sources through control of the dispersion units, acquires image data through the image sensor CCD and finally obtains molecular spectrum data of a sample. The molecular spectrum imaging device has high technical index and quick imaging speed, does not need a push-broom component, is easy to be combined with the general microscope for use and has wide imaging spectrum range covering visible light and near infrared light, thereby being favorable for detection and analysis of biological tissues.

The invention provides a fiber laser intensity noise suppressing device and a working method thereof. The device comprises a single mode semiconductor pump light source, a wavelength division multiplexer, a fiber resonator, a fiber coupler, a fiber isolator, a fiber circulator and a laser intensity noise suppressing device. Through a self-injection locking structure of a fiber laser, laser signals are injected into the fiber resonator after conventional intensity noise suppressing treatment, so that the finally output laser intensity noise is greatly reduced. The device with a relatively simple structure can realize high performance fiber laser output with ultralow intensity noise, thereby being of great importance for expansion of the application of fiber lasers in the field of high-precision optical fiber sensing, coherent light communication and atomic and molecular spectroscopy.

The embodiments of the invention disclose a spectral data analysis method and a spectral data analysis apparatus. The spectral data analysis method comprises: establishing a sample database, screeningthe neighboring spectral groups of a to-be-detected sample, carrying out dimension reduction on the neighboring spectral groups through cumulative contributing rate of principal component, determining target neighboring spectral groups according a Mahalanobis distance ratio, predicting the to-be-detected sample by selecting the target neighboring spectral group with the smallest relative prediction error as a training set sample, determining whether the representativeness of the sample database is sufficient, and adding the to-be-detected sample to the sample database if the representativeness of the sample database is insufficient. According to the present invention, the sample database is established by using the molecular spectroscopy technology, the optimal target neighboring spectralgroup is automatically selected from the sample database according to the spectral characteristics of the to-be-detected sample, the physical and chemical data of the to-be-detected sample is furtherpredicted, and the representativeness of the sample database is further determined, such that the automation of the spectral data analysis process and database update is achieved, the prediction accuracy is improved, the complicated work of manual establishing and model maintaining is eliminated, and the molecular spectroscopy technology is easily promoted and applied.

The invention relates to a system (1) for detecting analytes of interest present in a gas sample. The detection system comprises a layer (30) of porous material positioned along the fluidic flow path for the gas, the material containing probe molecules capable of reacting with the analyte of interest. The detection system also comprises an optical device (50) capable of successively detecting a change in the spectral properties of the probe molecules at different detection areas (32) of the porous material layer (30).

The light emission analyzing device includes: a first light intensity calculation unit that performs polynomial approximation on a spectroscopic spectrum indicating a light intensity for each wavelength in a container as measured by a spectrometer so as to calculate the light intensity; a second light intensity calculation unit that subtracts, for each wavelength, the light intensity calculated by the first light intensity calculation unit from the light intensity indicated by the spectroscopic spectrum measured by the spectrometer so as to calculate a light intensity corresponding to a bright-line spectrum of a molecule; and a ratio calculation unit that calculates, by using the light intensity calculated by the second light intensity calculation unit, a ratio between (a) a peak value of a molecular spectrum of a first molecule and (b) a peak value of a molecular spectrum of a second molecule.

Methods and apparatus in the field of single molecule sensing are described, e.g. for molecular analysis of analytes such as molecular analytes, e.g. nucleic acids, proteins, polypeptides, peptides, lipids and polysaccharides. Molecular spectroscopy on a molecule translocating through a solid-state nanopore is described. Optical spectroscopic signals are enhanced by plasmonic field-confinement and antenna effects and probed in transmission by plasmon-enabled transmission of light through an optical channel that overlaps with the physical channel.

The present invention relates to an Internet of things + molecular spectrum tumor clinical medical diagnosis method: a multivariate analysis method is used to establish a molecular spectrum database for tumor diagnosis and compile an Internet + Spectrum tumor clinical diagnosis software system. In clinical diagnosis, the patient's spectrum is compared with the molecular spectrum database of tumor pathological characteristics through the Internet to realize automatic and rapid clinical diagnosis of tumors. The invention is suitable for professional medical institutions to perform early prediction of clinical tumors on patients and real-time in-situ diagnosis during operation, and has the advantages of rapidity, considerable size, simple operation, and reduced pain of patients.

Systems and methods for spectrally resolved super-resolution microscopy (SRM) and ultrahigh-throughput single-molecule spectroscopy to synchronously and rapidly measure the fluorescence spectra and positions of millions of single molecules in dense samples.

Described herein is a spectroscopic system and method for measuring and monitoring the chemical composition and / or impurity content of a sample or sample stream using absorption light spectroscopy. Specifically, in certain embodiments, this invention relates to the use of sample pressure variation to alter the magnitude of the absorption spectrum (e.g., wavelength-dependent signal) received for the sample, thereby obviating the need for a reference or ‘zero’ sample. Rather than use a reference or ‘zero’ sample, embodiments described herein obtain a spectrum / signal from a sample-containing cell at both a first pressure and a second (different) pressure.

Light-emitting devices having an emitting layer containing a light-emitting organic or organometallic material and a nanostructure, the nanostructure having strong local electric fields at visible electromagnetic wavelengths that spectrally and spatially overlap with the light-emitting material. The spectral and spatial overlap of the electric fields of the nanostructure with the light emitting material uses high LDOS provided by the nanostructures to enable excited triplet electronic states in the material to emit light faster than without the nanostructure. This faster light emission from triplet-excited states leads to more stable emission from the light emitting material because it prevents buildup of triplet-excited states, which ordinarily can lead to quenching of light emission from the light emitting material. Among the many different possibilities contemplated, the nanostructure may advantageously be made of a dielectric material or a plasmonic metal material, such as SiO2, TiO2, ZnO, Al or Ag. It is further contemplated that the light-emitting material be capable of exhibiting at least one of phosphorescence or thermally-assisted delayed fluorescence. Many light-emitting materials, including blue light emitters, may be utilized, and may also be doped into a host material. It is still further contemplated that the nanostructure may be a nanoantenna, a nanoparticle, such as a sphere or rod, a nanoporous film, or an imprinted grating, and the nanostructure may be on either side of the light-emitting material, or may be surrounded by or embedded in the host material. The light-emitting device may also advantageously include other layers, including but not limited a hole transport layer, a hole blocking layer, an electron transport layer, a hole injection layer, or an electron injection layer. Further, the device may also be configured for use in various applications, including but not limited to bioimaging, photochemistry, and single molecule spectroscopy.

The invention relates to a microscope for the molecular spectroscopic analysis of a sample (2), having a beam path having at least one quantum cascade laser (QCL) (3) which emits an infrared (IR) radiation, a phase modulator (5) which is arranged between the QCL (3) and the sample (2), at least one optical element (6) which is arranged between the phase modulator (5) and the sample (2) and a sensor (4) which detects an IR radiation which is transmitted and / or reflected by the sample (2). The invention relates further to a method for the molecular spectroscopic analysis of a sample (2) comprising the steps of irradiating the sample (2) with an infrared (IR) radiation by means of a quantum cascade laser (QCL) (3), wherein the IR radiation is directed onto the sample (2) via a phase modulator (5) and at least one optical element (6), and detecting the IR radiation which is reflected and / or transmitted by the sample (2).

A label-free detection and characterization system includes an optical source; an optical path arranged to be optically coupled to the optical source; an optical resonator disposed proximate the optical path along a side of the optical path, the optical resonator having an optical whispering-gallery mode and being optically coupled to the optical path through an evanescent field to excite the optical whispering-gallery mode; an optical receiver arranged to be optically coupled to the optical path. The optical source is frequency locked to a resonance frequency of the optical resonator and provides light sufficiently intense to provide four-wave mixing while being coupled with the optical resonator resulting in a comb spectrum received by the optical receiver. The comb spectrum provides characteristic changes in the presence of a substance in contact with the optical resonator to provide detection and characterization of the substance.

The invention discloses a rapid nondestructive qualitative analysis method and device for fur. A fur detection device is developed based on molecular spectroscopy and chemometrics method, and rapid classification detection of fur is realized by spectral acquisition and data processing.

The invention belongs to the technical field of laser spectrum and provides a common NICE-OHMS (Noise-immune Cavity-Enhanced Optical Heterodyne Molecular Spectroscopy) system for detecting sub-Dopplerspectroscopy. The common NICE-OHMS system comprises a single side band phase modulator, an erbium-doped optical fiber amplifier and a voltage-controlled oscillator, wherein the voltage-controlled oscillator is used for driving the single side band phase modulator; a light beam emitted by a laser device passes through the single side band phase modulator, the erbium-doped optical fiber amplifier,an electro-optic modulator, a mode matching lens, a 1 / 2 wave plate, a polarized beam splitter prism and 1 / 4 wave plate in sequence and then enters a high-fineness cavity; a pre-cavity reflection signal of the high-fineness cavity is detected through a first detector and a reflection signal detected by a second detector is processed through a PID (Proportion Integration Differentiation) servo loopand then a feedback signal is output to the voltage-controlled oscillator so as to control the driving frequency of the voltage-controlled oscillator; furthermore, the off-set frequency of the singleside band phase modulator is controlled to realize PDH locking of laser frequency. The common NICE-OHMS system can be compatible with various laser devices and the compatibility of the NICE-OHMS system is improved.

A method for frequency-converting a source optical signal in order to produce a useful optical signal, by mixing a plurality of waves, implements a plurality of waveguides that are coupled together. Individual parameter values of the waveguides, as well as at least one coupling parameter, are selected so as to obtain the useful signal with a high intensity. Such a method for producing the useful signal is suitable for a spectroscopic application, in particular a molecular spectroscopy application.

The invention relates to a portable mid-infrared high-sensitivity multi-component gas measurement and analysis system and a working method thereof, and belongs to the technical field of laser measurement, the portable mid-infrared high-sensitivity multi-component gas measurement and analysis system comprises an optical parametric oscillator, an absorption cell and a data processing system; light of the optical parametric oscillator is divided into two paths through a beam splitter A, one path is connected with a data processing system and a digital lock-in amplifier through an absorption celland a detector A, and the digital lock-in amplifier is connected with the data processing system; the other path is connected with a beam splitter B and is divided into two paths, and the two paths are respectively connected to a data processing system through a detector B and a Michelson interferometer; the data processing system is further connected with a current driving module and a temperature control module, and the current driving module and the temperature control module are both connected with the optical parametric oscillator. According to the system, various narrow-molecule and wide-molecule spectral substances can be detected with high sensitivity in real time.

A device includes a substrate (100) that includes a resonant cavity (125). The resonant cavity (125) includes dipolar molecules that have an absorption frequency. The resonant cavity (125) resonates at a frequency that is equal to the absorption frequency of the dipolar molecules. The device further includes a first port (110) on the resonant cavity (125) configured to receive a radio frequency (RF) signal.

A method includes forming a plurality of layers of an oxide and a metal on a substrate. For example, the layers may include a metal layer sandwiched between silicon oxide layers. A non-conductive structure such as glass is then bonded to one of the oxide layers. An antenna can then be patterned on the non-conductive structure, and a cavity can be created in the substrate. Another metal layer is deposited on the surface of the cavity, and an iris is patterned in the metal layer to expose the one of the oxide layers. Another metal layer is formed on a second substrate and the two substrates are bonded together to thereby seal the cavity.

According to the Einstein relativism, mass measurement of an object equals measurement of energy therein. The working principle of an ultraviolet spectrum DNA and protein phenotype analyzer is to measure energy in DNA or protein, and includes an early-stage diagnosis method for tumor epigenetic electronic spectrum used in early-stage diagnosis of phymatology, and different cancer phenotype mass causes energy level change of atoms or molecules, and further causes displacement of the spectral line of an atomic or molecular spectrum. Different nuclear spinning causes change of the fine structureof the spettrum. The higher mass and momentum of the cancer phenotype correspond to shorter spectrum specificity wavelength (p=h / lambda), so that specific spectroscopic basis is provided for determining whether the tumor is benign or malignant. False positive tends not to occur in cancer epigenetic liquid DNA detection, advantages of epigenetic fingerprint screening enables that the technology canbe expected to be used in early-stage screening and prognosis treatment of tumors. The ultraviolet spectrum DNA and protein phenotype analyzer reforms early-stage diagnosis and treatment for tumors.

The invention provides a system for measuring water vapor temperature-variable absorption spectrum and its use method, including a difference frequency light source module, a difference frequency light source frequency monitoring and calibration module, a data acquisition and processing module, a three-light path temperature-controlled multi-pass pool device and a matching Gas module; the difference frequency light generated by the difference frequency light source module is introduced into the three-light path temperature-controlled multi-pass cell device, and the absorption information generated by laser injection into the sample cell is imported into the data acquisition and processing module. The gas distribution module is specifically a mixing container for mixing A certain proportion of water vapor and dry air, the data acquisition and processing module includes a computer with an integrated multi-spectral fitting program, by using the new BaGa 4 Se 7 The crystal is used as a frequency conversion device to generate 3~11μm mid-infrared difference frequency light, which is used in conjunction with a three-path temperature-controllable absorption cell, combined with a multi-spectral fitting program, to provide systematic and accurate basic experimental data for atmospheric detection and laser atmospheric transmission research. , can also provide an effective criterion for the improvement of the theoretical calculation model CRB of the spectral parameters of water molecules.

The invention provides a method for identifying tumor boundaries by performing molecular spectral imaging on cancer tissues by using a laser-induced breakdown spectroscopy technology, and belongs to the field of imaging. The method comprises the following steps: (1) carrying out molecular spectral imaging on a sample tissue by utilizing a laser-induced breakdown spectroscopy technology, and collecting an LIBS spectrum; (2) after performing baseline calibration on the LIBS spectrum, extracting a CN molecular spectral line or a C2 molecular spectral line to obtain an intensity relative value of the spectral line; (3) drawing a CN molecule or C2 molecule distribution heat map in the sample tissue; (4) carrying out clustering analysis on the CN molecule or C2 molecule distribution heat map to identify the boundary of the tumor region and the necrosis region in the sample tissue. By utilizing the method, the boundary of the tumor area and the necrosis area in the cancer tissue or para-carcinoma tissue of the lung cancer patient can be successfully displayed, and tissue space heterogeneity information is obtained. The method provided by the invention realizes molecular spectral imaging analysis of lung cancer tissues and tumor boundary identification, and has good application prospects and values.

Machine learning approach can combine mass spectral imaging (MSI) techniques, one with low spatial resolution but intact molecular spectra and the other with nanometer spatial resolution but fragmented molecular signatures, to predict molecular MSI spectra with submicron spatial resolution. The machine learning approach can perform transformations on the spectral image data of the two MSI techniques to reduce dimensionality, and using a correlation technique, find relationships between the transformed spectral image data. The determined relationships can be used to generate MSI spectra of desired resolution.