Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Soil DNA extraction method for analyzing microbial community structure of polycyclic aromatic hydrocarbon polluted land

A microbial community, polycyclic aromatic hydrocarbon technology, applied in the field of soil microbial molecular ecology, to achieve the effect of strong applicability, high safety and simple extraction method

Inactive Publication Date: 2017-10-17
BEIJING MUNICIPAL RES INST OF ENVIRONMENT PROTECTION
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported methods for extracting total DNA of microbial genomes from different environmental samples have certain limitations.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Soil DNA extraction method for analyzing microbial community structure of polycyclic aromatic hydrocarbon polluted land
  • Soil DNA extraction method for analyzing microbial community structure of polycyclic aromatic hydrocarbon polluted land
  • Soil DNA extraction method for analyzing microbial community structure of polycyclic aromatic hydrocarbon polluted land

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The content of the present invention will be further explained and illustrated below in conjunction with specific examples.

[0049] The test soil for the method of this embodiment is the soil on the polluted site of a comprehensive coking plant production enterprise. This enterprise uses coal as the main raw material and has produced gas, coke and coal chemical products since the 1950s. The incomplete combustion of fossil fuels in the production workshop and the processing of chemical products such as gas and coke may lead to the emission of polycyclic aromatic hydrocarbons. Yu Nian is a typical PAH-contaminated soil.

[0050] The reagent preparation method used

[0051] (1) β-mercaptoethanol-soil pretreatment buffer:

[0052] 50mM Tris, 20mM EDTA, 100mM NaCl, 10mg / mL PVP, pH10;

[0053] After preparation, add β-mercaptoethanol with a volume ratio of 1.5%, and mix well;

[0054] (2) 1×PBS buffer:

[0055] 8.00g NaCl, 0.20g KCl, 1.44g NaCl 2 HPO 4 , 0.24g KH 2 P...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a soil DNA extraction method for analyzing the microbial community structure of a polycyclic aromatic hydrocarbon polluted land. The method comprises the following specific steps: 1, preparation of a reagent; 2, extraction of a general DNA. The extraction method is simple, convenient to operate, high in applicability and high in safety. For the characteristics of severe polycyclic aromatic hydrocarbon pollution, long-term pollution history, high humus content and the like in soil, firstly, beta-mercaptoethanol with an anti-oxidization effect at a volume ratio of 1.5 percent is added into a soil pretreatment buffer solution to reduce influence of impurities in the soil on DNA extraction; then a relatively mild method for crushing microbial cells through mechanical force of glass beads is adopted, so that the DNA is fully released; furthermore, no high shear force is caused on the DNA, so that the extracted genome DNA is relatively high in intactness.

Description

technical field [0001] The invention belongs to the technical field of soil microbial molecular ecology, and in particular relates to a soil DNA extraction method for analyzing the microbial community structure of a polycyclic aromatic hydrocarbon polluted site. Background technique [0002] In the process of rapid industrialization in our country, due to the lack of people's awareness of environmental protection, the environment on which we live has been severely damaged. Soil, as a natural resource for human beings, has not been spared, and the problem of soil environmental pollution has become more and more acute. Among them, the pollution phenomenon of polycyclic aromatic hydrocarbons (Polycyclic aromatic hydrocarbons, polycyclic aromatic hydrocarbons) with strong carcinogenic, teratogenic and mutagenic effects is very common. Coal chemical companies and coking companies that use coal as the main raw material for production activities are one of the important factors tha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 张丹姜林夏天翔
Owner BEIJING MUNICIPAL RES INST OF ENVIRONMENT PROTECTION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com