Molecular standard sample of rape stem canker pathogen and its preparation method

Abstract

The invention belongs to the field of plant quarantine technology research. Molecular standard sample of canker canker of rapeseed. Rape canker sore molecular standard sample is prepared from high-purity DNA extracted from canker canker sore strain. The standard sample has the following physical and chemical properties: (1) shape: pure white powder; (2) each tube Content 5μg±0.5μg; (3) DNA purity: OD260 / 280=1.8~2; (4) Soluble in water or TE (Tris-EDTA) buffer. The rapeseed canker sore molecular standard sample of the present invention uses the rapeseed canker sore strain isolated by the laboratory as a raw material, and is cultured in large quantities under specific conditions to extract high-purity DNA, which is subpackaged and freeze-dried to obtain the rapeseed canker sore strain. Molecular standard product, with excellent physical and chemical properties, can be detected by PCR method with specific primers, and the sensitivity can reach 10-4 times. After testing, the standard product has good DNA integrity, uniformity and stability, and can be used in import and export testing as a positive reference substance to improve the accuracy of test results.

Description

technical field [0001] The invention relates to a molecular standard sample of canker sore pathogen of rapeseed, and also relates to a preparation method thereof, which belongs to the field of plant quarantine technology research. Background technique [0002] There are many reports on reference materials of animal viruses and food microorganisms at home and abroad, but the research on reference materials of plant pathogenic bacteria has just started, and many problems need to be solved urgently. In the detection of plant pathogens at home and abroad, most of the strains purchased from the American Type Culture Collection (ATCC) are used as positive controls. The price is very expensive, and it is almost impossible to buy them in China. However, there are few relevant research reports on the molecular DNA standard materials for the molecular detection of plant pathogens. Therefore, It is of great practical significance to conduct comprehensive and in-depth research on the pre...

AI Technical Summary

Problems solved by technology

In the detection of plant pathogens at home and abroad, most of the strains purchased from the American Type Culture Collection (ATCC) are used as positive controls, which are very expensive and almost impossible to purchase in China. However, there are few relevant research reports on molecular DNA standard materials for molecular detection of plant pathogens, so It is of great practical significance to conduct comprehensive and in-depth research on the preparation technology and stability assurance technology of DNA standard samples for the detection of plant pathogenic bacteria, actively carry out the development of molecular DNA standard samples for the detection of plant quarantine pathogens in my country, and fill in the gaps in this measurement field.

Images