Oral swab preservation solution as well as preparation method and application thereof

Abstract

An oral swab preservation solution comprises the following components: 5-50 mmol / L of Tris-HCl, 1-10 mmol / L of EDTA, 50 to 500 mmol / L of sodium chloride and 0.1 to 3% of SDS, wherein the pH range of the system ranges from 7 to 10. The oral swab preservation solution s clear in component, simple in preparation method, low in cost, good in stability and high in safety, free of toxic and side effects, easy to operate, convenient to store and transport, suitable for large-scale sampling and use, and wide in application prospect. The oral swab preservation solution is used for storing a human mouthswab sample, can be preserved at the temperature of 15-25 DEG C (room temperature.) for a long term, genomes in oral epithelial cells can be effectively protected from being damaged in a plurality ofweeks, the integrity of DNA (deoxyribonucleic acid) is good, and the oral swab sample is not polluted by microorganisms.

Description

technical field [0001] The invention relates to the technical field of cell preservation, in particular to a buccal swab preservation solution and its preparation method and application. Background technique [0002] DNA extraction is the most basic application technology in molecular biology testing. Traditionally, human whole blood is mostly used as the sample for gene extraction. There are certain difficulties in the sampling process, such as the damage of blood to the human body and the safety hazards in the process of blood collection. , Blood samples are easily contaminated, and the patient rejection rate is high. At the same time, the cost of taking blood samples is high, and the operation is relatively cumbersome. It requires professionally skilled personnel to operate it reliably. Therefore, it is of great significance to explore gene collection methods that are easier to obtain materials, more convenient to operate, and easier to be accepted by patients. [0003] ...

AI Technical Summary

Problems solved by technology

[0007] Existing patents are all saliva preservation liquid, which is consistent with the principle of oral swab preservation liquid, and its protective agent has complex components, such as patent publication numbers CN102440234B and CN102919218B

Wherein CN102919218B contains magnesium chloride, and magnesium ion is a nuclease activator, which easily causes the degradation of DNA, which is unfavorable for the preservation of the stability and integrity of DNA; CN102440234B contains protein denaturants such as guanidine isothiocyanate, which can inhibit nuclease activity, but at the same time, it also lyses the cells, leaving the DNA in a free state, which is not conducive to the integrity preservation of DNA

Containing sucrose and glucose components respectively in CN102919218B and CN105039306A, although can well keep the viscosity of this saliva preservation immobilization solution and maintain cell osmotic pressure, but easily cause the growth of microorganisms such as bacteria; The composition is complex in CN105368812A, although the composition such as urea, ethanol Organic substances such as mucin and globulin in saliva can be denatured, but at the same time, cells will be lysed, which is not conducive to the integrity of DNA, and these components provided in this application document are all necessary components, namely All components are indispensable to maintain the stability of DNA

[0008] In short, the existing technology has the following problems. The cryopreservation method can effectively inhibit the growth of microorganisms, but repeated freezing and thawing of oral epithelial cells can easily lead to cell lysis, which is not conducive to maintaining the stability of genetic material

Although normal saline preservation can maintain the stability of oral epithelial cell morphology, long-term storage is prone to the growth of bacteria, fungi and other microorganisms

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