Selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line

Abstract

The invention belongs to the technical field of rape breeding, and discloses a selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line (CMS). The method is characterized in that an artificially-bred turnip cabbage allotetraploid as a female parent is hybridized with a Brassica napus variety as a male parent, male sterile plants are isolated from the progeny of hybrid selfing, and a Brassica napus-turnip cabbage cytoplasmic male sterility line with genetic stability is obtained by successive backcross breeding. Restorer line and maintainer line relationship and molecular biological methods are employed to prove genetically and on DNA level respectively that the Brassica napus-turnip cabbage cytoplasmic male sterility line has a sterile cytoplasmic type different from those of Polima cytoplasmic male sterility (pol CMS), turnip cytoplasmic male sterility (ogu CMS, kos CMS) and Brassica juncea cytoplasmic male sterility (hau CMS). The invention adds a new rape cytoplasmic male sterility type for China and the rest of the world.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for selecting a cytoplasmic male sterile line of Brassica oleracea and radish, and belongs to a new breeding method and a new cytoplasmic male sterile line material of Brassica oleracea. Background technique [0002] At present, there are mainly the following types of pollen control systems for heterosis utilization in rapeseed at home and abroad: cytoplasmic male sterility (abbreviated as CMS), ecological cytoplasmic male sterility (abbreviated as ECMS), nuclear male sterility (abbreviated as GMS), and self-fertilization. Incompatibility (SI for short), chemical male sterility and transgenic male sterility, among which CMS is the most important pollen control system for the utilization of heterosis in rapeseed (Fu Tingdong et al. 1995). There are many types of rapeseed CMS that have been reported at home and abroad, and they can be divided into two...

AI Technical Summary

Problems solved by technology

There are many types of rapeseed CMS that have been reported at home and abroad, and they can be divided into two categories according to the source of their cytoplasm: one is the male sterility caused by mutations in the natural reproduction process of plants or hybridization between varieties, that is, homologous cytoplasmic male sterility , there are mainly Polima cytoplasmic male sterile (referred to as pol CMS), shan2A CMS and nap CMS and other types. This type of cytoplasmic male sterile is sensitive to temperature, has a small amount of pollen, and the sterility is unstable, thus affecting the purity of hybrids

The second type is the male sterility produced by distant hybridization or protoplast fusion, that is, heterologous cytoplasmic male sterility. There are mainly radish cytoplasmic male sterility (ogu cms and kos cms) and tour cms. This type of cytoplasmic male sterility Fertility is insensitive to temperature, has no pollen, and stable sterility, but it is difficult to discover and utilize restorer genes

In order to overcome the potential threat of single cytoplasmic cytoplasmic male sterile hybrids of rapeseed, researchers at home and abroad have discovered and created Nca cms (selected from the distant hybrids of Brassica napus and Ethiopian mustard) after years of research. , Cai Ming et al. 1993), Nsa cms (selected from somatic hybrid progenies of Xinjiang wild mustard and Brassica napus, Hu Qiong et al. 2004), Nea cms (produced by distant hybridization and radiation mutagenesis, Jiang Liangcai et al. 2002) and hau cms (natural wild mustard rape male sterility) and other new types of cytoplasmic male sterility, but these research results are only limited to the resource research stage, far from practical application

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