Selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line

A cabbage rape, male sterile line technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of difficulty in discovering and utilizing restoration genes, far from practical applications, affecting the purity of hybrids, etc. , to achieve the effects of convenient breeding research and utilization, simple breeding procedures and clear breeding goals

Inactive Publication Date: 2012-05-30
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many types of rapeseed CMS that have been reported at home and abroad, and they can be divided into two categories according to the source of their cytoplasm: one is the male sterility caused by mutations in the natural reproduction process of plants or hybridization between varieties, that is, homologous cytoplasmic male sterility , there are mainly Polima cytoplasmic male sterile (referred to as pol CMS), shan2A CMS and nap CMS and other types. This type of cytoplasmic male sterile is sensitive to temperature, has a small amount of pollen, and the sterility is unstable, thus affecting the purity of hybrids
The second type is the male sterility produced by distant hybridization or protoplast fusion, that is, heterologous cytoplasmic male sterility. There are mainly radish cytoplasmic male sterility (ogu cms and kos cms) and tour cms. This type of cytoplasmic

Method used

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  • Selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line
  • Selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line
  • Selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Breeding of Cytoplasmic Male Sterile Lines of Brassica oleracea Radish Brassica

[0030] Breeding steps of the cytoplasmic male sterile line NRO4270A of Brassica napus radish and Brassica oleracea:

[0031] Depend on figure 1 Shown:

[0032] (1) Heqing radish (Raphanus sativus L, 2n=18, RR) was used as the female parent and Brassica alboglabra Bailey (2n=18, CC) was used as the male parent to perform hybridization to obtain the first generation of hybrids, and sow the first generation of hybrids, The first generation seedlings of the hybrid were treated with 0.3% colchicine to obtain radish and cabbage allotetraploid (Raphanobrassica, RRCC, 2n=36) (Chen Honggao, Wu Jiangsheng, etc., Synthesis of radish-kale allotetraploid and GISH Analysis of Crops, 2006, 32 (8)); with the radish and cabbage heterotetraploid as the female parent, the Brassica napus variety YD16 (Zhao Heju et al., rapeseed, Chinese cabbage and shepherd's purse cross-breeding new varieties wi...

Embodiment 2

[0039] Example 2: Genetic classification of the cytoplasmic male sterile line NRO4270A of Brassica napus radish

[0040] 1. Determination of recovery relationship:

[0041] (1) Eight representative cultivars or lines were selected for test crossing with pol CMS, ogu CMS, kos CMS, hau CMS and NRO4270A CMS materials in Brassica napus.

[0042] Table 2 Determination results of recovery relationship between NRO4270A CMS and several rapeseed CMS materials

[0043]

[0044]

[0045] Note: (1) S in the table means sterile, and F means fertile.

[0046] (2) The test cross materials listed in Table 2 are the effects of testing the degree of recovery and maintenance of the materials of the present invention, and are not inventions and creations completed by relying on the test cross materials (as long as the existing breeding resources in China have the same genetic characteristics of the above materials as this invention) testcross material for the inventive step, so this examp...

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Abstract

The invention belongs to the technical field of rape breeding, and discloses a selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line (CMS). The method is characterized in that an artificially-bred turnip cabbage allotetraploid as a female parent is hybridized with a Brassica napus variety as a male parent, male sterile plants are isolated from the progeny of hybrid selfing, and a Brassica napus-turnip cabbage cytoplasmic male sterility line with genetic stability is obtained by successive backcross breeding. Restorer line and maintainer line relationship and molecular biological methods are employed to prove genetically and on DNA level respectively that the Brassica napus-turnip cabbage cytoplasmic male sterility line has a sterile cytoplasmic type different from those of Polima cytoplasmic male sterility (pol CMS), turnip cytoplasmic male sterility (ogu CMS, kos CMS) and Brassica juncea cytoplasmic male sterility (hau CMS). The invention adds a new rape cytoplasmic male sterility type for China and the rest of the world.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for selecting a cytoplasmic male sterile line of Brassica oleracea and radish, and belongs to a new breeding method and a new cytoplasmic male sterile line material of Brassica oleracea. Background technique [0002] At present, there are mainly the following types of pollen control systems for heterosis utilization in rapeseed at home and abroad: cytoplasmic male sterility (abbreviated as CMS), ecological cytoplasmic male sterility (abbreviated as ECMS), nuclear male sterility (abbreviated as GMS), and self-fertilization. Incompatibility (SI for short), chemical male sterility and transgenic male sterility, among which CMS is the most important pollen control system for the utilization of heterosis in rapeseed (Fu Tingdong et al. 1995). There are many types of rapeseed CMS that have been reported at home and abroad, and they can be divided into two...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 吴江生石淑稳刘克德王小峰刘超
Owner HUAZHONG AGRI UNIV
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