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Simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines

A male sterile line and cytoplasmic technology, which is applied in the field of plant cultivation, can solve the problems of labor and time-consuming, lack of practical and easy-to-use cabbage cross-breeding methods, and high cost

Active Publication Date: 2012-07-04
YUNNAN SINONG VEGETABLES SEED
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of self-incompatible lines for seed production has the following disadvantages: it takes a lot of manpower and time to reproduce the parents through artificial pollination at the bud stage, the cost is high, and the promotion area is also limited by the number of seeds produced
The above methods all need to carry out backcross breeding for more than 5 generations first, and then prepare hybrids, which is labor-intensive and time-consuming, and lacks practical and simple new cabbage hybrid breeding methods

Method used

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  • Simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines
  • Simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines
  • Simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines

Examples

Experimental program
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Effect test

Embodiment 1

[0027] In this embodiment, Hugan No. 1 (purchased from Shanghai Yihan Seedling Trading Co., Ltd.) is used as the female parent, and the young lateral buds are taken from the plant in spring, preserved and multiplied by tissue culture in the laboratory, and stable ones are selected. The cabbage inbred line 03-2-1-3-2 was the male parent and a hybrid was prepared according to the above method, and the obtained hybrid was coded as Ruigan 60.

Embodiment 2

[0029] In this embodiment, Zhonggan 22 (purchased from Jiutou Niaocai Seed Line, Wuchang District, Wuhan City) is used as the female parent, and the young lateral buds are taken from the plant in spring, preserved and multiplied by tissue culture in the laboratory, and selected stable The cabbage inbred line 03-3-6-2-1-1 was used as the male parent to prepare a hybrid according to the above method, and the hybrid number obtained was Ruigan 62.

Embodiment 3

[0031] In this embodiment, Zhonggan 22 (purchased from Jiutou Niaocai Seed Line, Wuchang District, Wuhan City) is used as the female parent, and the young lateral buds are taken from the plant in spring, preserved and multiplied by tissue culture in the laboratory, and selected stable The cabbage inbred line 04-1-5-7-2 was used as the male parent to prepare a hybrid according to the above method, and the hybrid number obtained was Ruigan 58.

[0032] For the hybrid varieties obtained in each of the above examples, the results of field observation and identification showed that the yield and disease resistance of the three new hybrid combinations showed superparental dominance, see Table 2 for details. The varieties "Xiaguang" (early maturing oblate type) and "Meimei Zaosheng" (early maturing spherical type) were purchased on the market as contrasts, compared with the varieties of the three embodiments in terms of yield and disease resistance, the results are shown in the table ...

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Abstract

The invention relates to a breeding method of hybrid cabbage, in particular to a simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines, which belongs to the technical field of plant culture. The invention directly adopts the cabbage varieties of Zhonggan No.22 and Hugan No.2 prepared by cytoplasmic male sterile lines sold in the market as maternal plants,and adopts cabbage inbred lines (with the same ball shape as the maternal plants, the same type of leaf ball mature performance as the maternal plants and stable botanical characters) as pollen suppliers for preparing good coenospecies. The invention has the advantages that when the advantages of using the cytoplasmic male sterile lines for preparing the cabbage coenospecies are maintained, good cabbage inbred lines do not need to be used as recurrent parents for fifth to seventh generation backcrossing and transformation with male sterilant materials, the labor is saved, the cost is saved, the breeding time is greatly shortened, and in addition, the hybrid vigour parts of the original varieties can be maintained into the new hybrid varieties.

Description

technical field [0001] The invention relates to a breeding method of hybrid cabbage, in particular to a simplified breeding method for preparing hybrid cabbage by using a cytoplasmic male sterile line, and belongs to the technical field of plant cultivation. Background technique [0002] Cabbage (Brassica oleracea var. capitata L.) is the main vegetable crop in China and many countries in the world. Because of its obvious heterosis, heterosis breeding has become the main way of cabbage breeding. At present, the excellent cabbage varieties used in production are almost all the first-generation hybrids, and there are two main ways to produce the first-generation hybrids of cabbage, that is, using self-incompatible lines to produce seeds and using male sterile lines to produce seeds. Seed production using self-incompatible lines has long been the main method for preparing the first generation of hybrids in cabbage. Great achievements have been made through this approach, and a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 戴忠良秦文斌潘跃平姚悦梅毛忠良吴国平潘永飞张振超王建华肖燕
Owner YUNNAN SINONG VEGETABLES SEED
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