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A Breeding Method for Quickly Obtaining Cytoplasmic Male Sterile Line and Maintainer Line of Rouge

A technology for cytoplasmic male sterility and rouge radish, which is applied in the directions of botanical equipment and methods, application, and plant genetic improvement, can solve the problems of the male sterile line and maintainer line selection and breeding process not being elaborated, and achieve heterosis. Significant, uniform botanical traits, stable botanical traits

Inactive Publication Date: 2016-07-06
单县秋歌农产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lu Fafa et al. (Chinese Vegetables, 2012(2): 104-106) and Tao Hongying et al. (Chinese Vegetables, 2012(1): 37) respectively prepared Rouge 1 and Rouge 2 Red Heart by using the male sterile lines selected. Radish hybrids, but the selection process for male sterile lines and maintainer lines is not elaborated

Method used

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  • A Breeding Method for Quickly Obtaining Cytoplasmic Male Sterile Line and Maintainer Line of Rouge
  • A Breeding Method for Quickly Obtaining Cytoplasmic Male Sterile Line and Maintainer Line of Rouge
  • A Breeding Method for Quickly Obtaining Cytoplasmic Male Sterile Line and Maintainer Line of Rouge

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The self-retained species "Subei Carrot" from Jiangsu was selected, with a flat leaf type, rich in red pigment in the fleshy root bark and fleshy part; the radish cytoplasmic male sterile line D-1 was selected as the sterile source, with a flat leaf type, Red skin and white heart.

[0028] 1. After the rouge radish variety "Subei Carrot" is sown, observe the separation and change of offspring traits after the first generation of selfing. A good breeding goal is to select a single plant and save the seed for the second generation of selfing.

[0029] 2. In the second-generation population of self-crossing, according to the breeding goal, continue to select individual plants for self-purification, and select individual plants for the third-generation self-crossing.

[0030] 3. At the same time, according to the breeding goal, look for the radish cytoplasmic male sterile line material D-1 of the same species that is closer to the target traits as the female parent, and us...

Embodiment 2

[0039] The commercial variety "Ranzhihong No. 1" from Sichuan was selected, with split-leaf type, rich in red pigment in the fleshy root bark and fleshy part; the radish cytoplasmic male sterile line material C-1 was selected as the sterile source, with split-leaf type, White skin and white heart.

[0040] 1. After the rouge radish variety "Ranzhihong No. 1" is sown, observe the separation and change of offspring traits after the first generation of self-crossing. The goal of breeding with good compatibility is to select a single plant and save the seed for the second generation of selfing.

[0041] 2. In the second-generation population of self-crossing, according to the breeding goal, continue to select individual plants for self-purification, and select individual plants for the third-generation self-crossing.

[0042]3. At the same time, according to the breeding goal, look for the radish cytoplasmic male sterile line material D-1 of the same species that is closer to the...

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Abstract

The invention discloses a breeding method for quickly obtaining the cytoplasmic male sterile line and maintainer line of rouge radish. It mainly selects different types of rouge radish materials, determines the breeding target after the first generation of self-crossing, selects a single plant directionally, and conducts the second generation. Generation selfing; select individual plants in the self-fertilized offspring group to continue selfing, and select individual plants according to the breeding goal; at the same time, select the cytoplasmic male sterile line as the female parent, and use the selected individual plant as the male parent to carry out Interspecies crosses to obtain hybrid F1, thereby transferring the CMS gene of the sterile line to kale; the selfed offspring continue to self-purify, and at the same time, the screening in the cytoplasmic male sterile hybrid plant population is closest to the male parent's breeding target As the female parent, the single plant that is the closest to the breeding target selected from the selfed offspring is the male parent and continues to be paired for hybridization; the purification of the selfed offspring and the transfer of male sterility, each generation is paired at the same time After several generations of selfing, maintainer lines and male sterile lines can be obtained. The method of the invention can significantly save labor and cost, and shorten the breeding time.

Description

technical field [0001] The invention relates to a breeding method of rouge radish, in particular to a breeding method for quickly obtaining a cytoplasmic male sterile line and a maintainer line of rouge radish, and belongs to the technical field of plant breeding. Background technique [0002] Rouge radish (Raphanus Sativus L. Sinoruber Makino), also known as red radish, belongs to a local species of radish in the genus Brassicaceae. It is famous for its full red carpel and is rich in anthocyanins. Sichuan, Guizhou and other provinces are also cultivated. In recent years, many units have extracted natural anthocyanins from the fleshy root of rouge radish. Radish red pigment is a kind of natural food coloring, which is safe, non-toxic, rich in resources, and has certain nutritional and pharmacological effects. It is one of the natural pigments allowed to be used by the National Additive Standards Committee. It has the characteristics of good chemical stability, storage stab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 张振超毛忠良潘跃平吴国平王建华戴忠良秦文斌姚悦梅潘永飞肖燕孙春青马志虎孙国胜
Owner 单县秋歌农产品有限公司
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