A Breeding Method for Quickly Obtaining Cytoplasmic Male Sterile Line and Maintainer Line of Rouge

Abstract

The invention discloses a breeding method for quickly obtaining the cytoplasmic male sterile line and maintainer line of rouge radish. It mainly selects different types of rouge radish materials, determines the breeding target after the first generation of self-crossing, selects a single plant directionally, and conducts the second generation. Generation selfing; select individual plants in the self-fertilized offspring group to continue selfing, and select individual plants according to the breeding goal; at the same time, select the cytoplasmic male sterile line as the female parent, and use the selected individual plant as the male parent to carry out Interspecies crosses to obtain hybrid F1, thereby transferring the CMS gene of the sterile line to kale; the selfed offspring continue to self-purify, and at the same time, the screening in the cytoplasmic male sterile hybrid plant population is closest to the male parent's breeding target As the female parent, the single plant that is the closest to the breeding target selected from the selfed offspring is the male parent and continues to be paired for hybridization; the purification of the selfed offspring and the transfer of male sterility, each generation is paired at the same time After several generations of selfing, maintainer lines and male sterile lines can be obtained. The method of the invention can significantly save labor and cost, and shorten the breeding time.

Description

technical field

[0001] The invention relates to a breeding method of rouge radish, in particular to a breeding method for quickly obtaining a cytoplasmic male sterile line and a maintainer line of rouge radish, and belongs to the technical field of plant breeding. Background technique

[0002] Rouge radish (Raphanus Sativus L. Sinoruber Makino), also known as red radish, belongs to a local species of radish in the genus Brassicaceae. It is famous for its full red carpel and is rich in anthocyanins. Sichuan, Guizhou and other provinces are also cultivated. In recent years, many units have extracted natural anthocyanins from the fleshy root of rouge radish. Radish red pigment is a kind of natural food coloring, which is safe, non-toxic, rich in resources, and has certain nutritional and pharmacological effects. It is one of the natural pigments allowed to be used by the National Additive Standards Committee. It has the characteristics of good chemical stability, storage stab...

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