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Molecule identification and transfer technology for broad-spectrum rice-blast resistant gene of paddy rice

A rice blast resistance gene and broad-spectrum technology, which is applied in the direction of microbial determination/testing, biochemical equipment and methods, can solve the problems of loss of variety utilization value, rice blast resistance does not have broad-spectrum, etc., and improve breeding efficiency , the effect of prolonging the use value

Inactive Publication Date: 2006-10-04
CHINA NAT RICE RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, several blast resistance genes such as Pi-ta and Pi-b have been cloned from rice, but these cloned genes do not have broad-spectrum resistance to rice blast, and germplasm carrying such genes have long been lost varieties in China Utilization value of improved disease resistance

Method used

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  • Molecule identification and transfer technology for broad-spectrum rice-blast resistant gene of paddy rice
  • Molecule identification and transfer technology for broad-spectrum rice-blast resistant gene of paddy rice
  • Molecule identification and transfer technology for broad-spectrum rice-blast resistant gene of paddy rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The acquisition of "Gumei No. 4"

[0022] my country's own rice seed resources, on the basis of natural or induced disease in multi-point diseased areas for many years, are selected from rice germplasm resources through artificial inoculation with different domestic strains of Magnaporthe oryzae as the main and foreign strains as supplementary. Gumei No. 4";

[0023] (2) Carry out gene mapping on the segregation population constructed by crossing Gumei No. 4 with the susceptible variety, in the region of approximately 70 kb near the centromere of the sixth chromosome, its sequence is shown in SEQ ID No: 1;

[0024] The full length of SEQ ID No: 1 is 70798bp.

Embodiment 2

[0026] The disease-resistant gene Pi-gm(t) or Pi-2 or Pi-9 of Gumei No. 4 was finely mapped to the 70kb region of SEQ ID No: 1, and six disease-resistant gene clusters were identified in the SEQ ID No: Six PCR-based molecular markers SEQ ID Nos: 2, 3, 4, 5, 6, and 7 at both ends and the middle of 1.

[0027] molecular marker

[0028] Among them, SEQ ID No: 2, 3, 6, 7 need enzyme digestion.

Embodiment 3

[0030] Varieties with disease-resistant loci developed using marker-assisted selection derived from molecular marker design; such as figure 1 , figure 2 , image 3 As shown, the use of these molecular markers can quickly identify whether the disease-resistant loci exist, and the plants with the disease-resistant loci can continue to improve the target traits, so that the breeding period can be shortened, and compared with the current conventional breeding, the breeding process is accelerated. process, saving a lot of manpower, material and financial resources.

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Abstract

The related rice gene mark for broad spectrum anti rice blast includes mark length 10-30 nucleotides with sequence same as the sequence of 1st-70798th in SEQ ID No:1. Wherein, based on our rice resource and natural or induced diseases, it applies different rice blast plants with the domestic as main and foreign as assist to screen the 'Gumei 4th', uses molecular mark technique to locate gene and obtain Pi-gm(t) gene with chain mark, and has great application value in breeding.

Description

technical field [0001] The invention belongs to the technical field of identifying broad-spectrum rice blast resistance genes by using molecular biology methods. It relates to a nucleotide sequence molecular marker that can be used to identify a broad-spectrum rice blast resistance gene, and a disease-resistant gene and a disease-resistant variety obtained from the molecular marker. Background technique [0002] Rice is an important economic crop, which is related to the national economy and people's livelihood; rice blast is one of the most common and most destructive fungal diseases in rice production in my country, and it is also the cause of serious rice production reduction after the disease occurs in major rice-producing countries in the world. major disease. From 1975 to 1990, 157 million tons of rice were lost worldwide due to rice blast. According to the incomplete statistics of the Plant Protection Center of the Ministry of Agriculture of China, from 1987 to 1997,...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 朱旭东邓一文陈红旗何祖华
Owner CHINA NAT RICE RES INST
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