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Human papillomavirus type bivalent virus-like particle mixed protein antigen and construction method

A technology of human papillomavirus and protein antigen, applied in antiviral agents, pharmaceutical formulations, animal/human peptides, etc., can solve the problems of high recurrence rate of condyloma acuminatum and lack of treatment methods

Inactive Publication Date: 2005-10-19
HARBIN MEDICAL UNIVERSITY
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of ideal treatment, the recurrence rate of genital warts is as high as 30% to 70%

Method used

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  • Human papillomavirus type bivalent virus-like particle mixed protein antigen and construction method

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Experimental program
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Effect test

specific Embodiment approach 1

[0005] Embodiment 1: The bivalent virus-like particle mixed protein antigen of this embodiment includes a L1 antigen of a cervical cancer-causing virus HPV16 and a L1 antigen of a condyloma acuminatum virus HPV11.

specific Embodiment approach 2

[0006] Embodiment 2: This embodiment is different from Embodiment 1 in that it also includes a vector, which is a Bacmid plasmid.

specific Embodiment approach 3

[0007] Specific embodiment three: In this embodiment, the bivalent virus-like particle mixed protein antigen is constructed according to the following method: the HPV11L1 gene is cloned from condyloma acuminata tissue by molecular biology methods, and the HPV16L1 gene and HPV11L1 gene are double-expressed in baculovirus insect cells Expression system to construct recombinant virus strain Bacmid / HPV16-HPV11L1.

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Abstract

The present invention relates to human papillomavirus type bivalent virus-like particle mixture, and human papillomavirus type bivalent virus-like particle mixing protein antigen and its construction method. The present invention includes one kind of cervical carcinoma virus HPV16 L1 antigen and one kind of condyloma acuminata virus HPV11 L1 antigen. The HPV11 L1 gene is first cloned from condyloma acuminata tissue through molecular geological method, and both HPV16 L1 gene cloned from cervical carcinoma tissue and the HPV11 L1 gene are then expressed in the insect cell expression system of baculovirus, so as to constitute recombinant virus strain Bacmid / HPV16-HPV11L1. Animal immunizing test shows that the constituted recombinant protein has excellent immunogenicity and immunoreactivity, and may be used as candidate antigen of gene engineering multivalent vaccine for preventing condyloma acuminata and cervical carcinoma.

Description

Technical field: [0001] The invention relates to a mixture of human papillomavirus-type bivalent virus-like particles and a method for constructing the mixture. Background technique: [0002] Condyloma acuminatum is a serious threat to human health as a sexually transmitted disease, and its incidence rate has accounted for the first place among sexually transmitted diseases in my country. Due to the lack of ideal treatment, the recurrence rate of genital warts is as high as 30% to 70%. Therefore, seeking ideal treatment and prevention methods should become an urgent matter in our country. In my country, the incidence of cervical cancer ranks second among gynecological tumors, with about 130,000 new cases each year. As a developing country in China, the incidence of cervical cancer remains high due to the lack of popularization of clinical exfoliated cell screening. [0003] The high-risk type HPV16 of human papillomavirus (HPV) causes cervical cancer (about 50%); the low-...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/20C07K14/435C12N15/09
Inventor 谷鸿喜庄敏魏兰兰商庆龙李迪王燕张凤民
Owner HARBIN MEDICAL UNIVERSITY
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