DNA (Deoxyribonucleic Acid) molecular identification method for Luchuan pigs and meat products thereof

A technology of DNA molecules and identification methods, which is applied in the field of DNA molecular identification of Luchuan pigs and their meat products, to achieve the effects of enriching polymorphisms, improving detection efficiency and accuracy, and requiring less templates

Active Publication Date: 2013-07-24
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report on the application of DNA molecular technology to distinguish and identify Luchuan pig breeds at home and abroad

Method used

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  • DNA (Deoxyribonucleic Acid) molecular identification method for Luchuan pigs and meat products thereof
  • DNA (Deoxyribonucleic Acid) molecular identification method for Luchuan pigs and meat products thereof
  • DNA (Deoxyribonucleic Acid) molecular identification method for Luchuan pigs and meat products thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Identify and distinguish Luchuan pigs and their meat products from binary cross pigs, ternary cross pigs and their meat products

[0036] Several samples of fresh meat, bacon and cut meat from Luchuan pigs, binary cross pigs, and ternary cross pigs to be identified were selected, and the DNA of each sample was extracted by the improved SDS method.

[0037] Using WM14 as primer, through ISSR-PCR reaction, the total DNA of each group of samples was amplified. ISSR-PCR reaction system is a 25μL system: template DNA 1μL, primers 2μL, 2×Taq PCR Master Mix 12.5μL, ddH 2 O9.5μL, where 2×Taq PCR Master Mix contains Taq DNA polymerase, dNTPs, MgCl 2 , Reaction buffer, PCR reaction enhancer and optimizer and stabilizer, the concentration is 2×, and does not contain dye; amplification program: 95℃ pre-denaturation for 5min, 95℃ denaturation for 30s, 36℃ annealing for 30s, 72℃ extension 2min, 30 cycles, total extension at 72℃ for 5min. Among them, the Marker is a 100bp Plus DNA ladder,...

Embodiment 2

[0044] Identify and distinguish between Luchuan pig and its meat products and Huanjiang pig fresh meat and its meat products

[0045] Several samples of fresh meat and bacon of Luchuan pig and Huanjiang pig were selected to be identified, and the DNA of each sample was extracted by the improved SDS method.

[0046] Using WM12 as the primer, ISSR-PCR amplification was the same as in Example 1.

[0047] Use 1.5% agarose gel, 1×TAE buffer as the medium electrophoresis to detect the amplification results, draw electrophoresis patterns 4 and 5 according to the electrophoresis diagram, Figure 4 The electrophoresis pattern drawing based on Picture 9 . As shown by the arrows in each figure, the Huanjiang Xiang pig samples produced a clearer band, but there is no such band in Luchuan pigs. Repeated verification experiments can prove that primer WM12 can be used to distinguish between Luchuan pigs and Huanchuan pigs. Strong evidence of Jiangxiang pig breed.

[0048] among them Figure 4 Midd...

Embodiment 3

[0053] Identify and distinguish between Luchuan pig and its meat products and Bama Xiang pig fresh meat and its meat products

[0054] Several samples of fresh meat and cut meat samples of Luchuan pig and Bama pig to be identified were selected, and the DNA of each sample was extracted by the improved SDS method.

[0055] Using WM29 as the primer, ISSR-PCR amplification was the same as in Example 1.

[0056] Use 1.5% agarose gel, 1×TAE buffer solution as the medium electrophoresis to detect the amplification results, draw electrophoresis patterns 6 and 7 according to the electrophoresis diagram, and Figure 7 The electrophoresis pattern drawing based on Picture 10 . As shown by the arrows in each figure, the Bama fragrant pig samples produced a clearer band, but there is no such band in Luchuan pigs. Repeated verification experiments can prove that primer WM29 can be used to distinguish between Luchuan pigs and Pakistani pigs. Strong evidence of Maxiang pig breed.

[0057] among th...

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Abstract

The invention relates to a DNA (Deoxyribonucleic Acid) molecular identification method for Luchuan pigs and meat products thereof. According to the method, primers used in ISSR-PCR (Inter-Simple Sequence Repeat-Polymerase Chain Reaction) are WM14, WM12 and WM29, and nucleotide sequences of the primers are 5'-CTCCTCCTCGC-3', 5'-CACCACCACGC-3' and 5'-CTCCTCCTCAT-3'; and finally, seven electrophoretograms of the Luchuan pigs and the meat products thereof are drawn, a DNA fingerprint atlas of the Luchuan pigs and the meat products thereof is established, and the electrophoretograms and the DNA fingerprint atlas can serve as a basis for the distinction and identification of the Luchuan pigs and the meat products thereof. The method has the advantages that a reliable DNA molecular identification technique is adopted, so that the differences among the Luchuan pigs, the meat products of the Luchuan pigs, Luchuan pig hybrids, meat products of the Luchuan pig hybrids, Bama miniature pigs, meat products of the Bama miniature pigs, Huanjiang miniature pigs and meat products of the Huanjiang miniature pigs are identified quickly and accurately, and the development of advantaged brand pigs, namely the Luchuan pigs, is promoted.

Description

Technical field [0001] The invention relates to a method for identifying pig breeds using DNA molecular marking technology, in particular to a DNA molecular identification method for Luchuan pigs and their meat products. Background technique [0002] Luchuan pig is an excellent pig breed in South China. It is named after it was originally produced in Luchuan County in the southeast of Guangxi Province. It is now mainly distributed in Guangxi Yulin, Wuzhou, Qinzhou and other regions. Luchuan pigs, along with Rongchang pigs, Taihu pigs, Dongbei min pigs, Jinhua pigs, Ningxiang pigs, Huai pigs and Bamei pigs, are collectively called "China's top eight local excellent pig breeds". Luchuan pig is a small fat pig breed, which has a long-standing reputation for its thin skin, tender meat, crisp but not greasy, delicious taste and rich nutrition. Its livestock products can be processed into sausages, skinless pork belly, white-cut pork knuckles, crispy suckling pig, buckle meat and othe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 韦保耀王淼黄丽滕建文夏宁王勤志封毅
Owner GUANGXI UNIV
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