Mycoplasma bovis immunity-related protein P22, nucleotide sequence for coding same and application thereof

A nucleotide sequence, Mycoplasma bovis technology, applied in the field of biomedicine, can solve the problems of less research on the immunogenicity and immune protection characteristics of Mycoplasma bovis components, and the lack of obvious immunogenicity of VSP protein

Inactive Publication Date: 2012-08-01
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In 2000, the epitope map of VSP protein and adhesion function was published, but the immunogenicity of VSP protein is not obvious, so it is speculated that there must be another imm

Method used

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  • Mycoplasma bovis immunity-related protein P22, nucleotide sequence for coding same and application thereof
  • Mycoplasma bovis immunity-related protein P22, nucleotide sequence for coding same and application thereof
  • Mycoplasma bovis immunity-related protein P22, nucleotide sequence for coding same and application thereof

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Experimental program
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Effect test

Embodiment 1

[0033] 1 Materials and methods

[0034] 1.1 Strain and serum

[0035] M.bovis Hubei-1 isolate (XIN Jiu-qing 1* , LI Yuan 1 , GUO Dan 1 et al., First isolation of Mycoplasma bovis from calf lung with pneumoniae in China. Chinese Journal of Preventive Veterinary Medicine: 2008-09) was isolated from disease materials submitted by our research group from Hubei Province. After gene sequence detection and biochemical tests, it was identified as Mycoplasma bovis. Sera Nos. 1 and 2 used in the ELISA test came from bovine serum that was naturally infected and finally confirmed to be Mycoplasma bovis by pathogen isolation; blood No. 3 and No. 4 were healthy bovine serum produced by Hangzhou Sijiqing Bioengineering Co., Ltd. and used for cell culture. Serum Nos. 5 to 7 are hyperimmune serum obtained from challenged healthy cattle with Mycoplasma bovis isolate Hubei-1; serum No. 8 is CBPP standard positive serum from CBPP International Reference Laboratory.

[0036] 1.2 Main reagent...

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Abstract

The invention discloses a mycoplasma bovis immunity-related protein P22, a nucleotide sequence for coding the same and an application thereof. A mycoplasma bovis Hubei strain is taken as a sample, and an efficient two-dimensional electrophoresis system of a mycoplasma bovis whole protein is established, so that a two-dimensional electrophoretogram with high resolution and high repeatability is obtained; an immunity-related protein is screened in combination with Western blot, and is named P22 protein; and as proved by a prokaryotic expression result of the protein, a Western blot rest result of a recombinant protein and a mycoplasma bovis positive serum is negative, and a Dot blot test result is positive. The protein is proved to be possibly a conformation-dependent immunity-related protein of mycoplasma bovis, and the mycoplasma bovis immunity-related protein P22 plays a guidance role in diagnosing, preventing and treating a mycoplasma bovis disease.

Description

technical field [0001] The invention discloses an immune-related protein, a nucleotide sequence encoding it and its application, and particularly relates to a protein P22 related to the immunity of Mycoplasma bovis, a nucleotide sequence encoding it and preparation of a reagent for detecting Mycoplasma bovis infection in the application. Belongs to the field of biomedicine. Background technique [0002] Mycoplasma bovis (Mycoplasma bovis, M.bovis) belongs to Mollicute, Mycoplasmales, Mycoplasmataceae, Mycoplasma, is bovine pneumonia, arthritis, mastitis, keratoconjunctivitis , and some diseases such as genital tract injury, and can cooperate with other pathogens to cause secondary infection. Since American Hale isolated Mycoplasma bovis from milk for the first time in 1961, Mycoplasma bovis has spread rapidly, causing huge economic losses to the United Kingdom and the United States. In 2008, the pathogen M.bovis was isolated from respiratory disease cases for the first ti...

Claims

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Application Information

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IPC IPC(8): C07K14/30C12N15/31C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10C12Q1/68C12Q1/04G01N33/68
Inventor 辛九庆李媛刘洋
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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