Method for identifying immunofixation electrophoresis M protein components by using computer
An immunofixation electrophoresis and computer technology, which is applied in calculation, electrical digital data processing, special data processing applications, etc., and can solve problems such as subjective judgment errors
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Embodiment 1
[0104] Embodiment 1 Utilizes computer to identify M proteinemia sample
[0105] (1) Immunofixation electrophoresis assay and scanning
[0106] 1. Methodological principle: Immunofixation eletrophoresis (IFE) performs zone electrophoresis on serum or other specimens on agar (cellulose acetate) plates, and various single proteins or composite protein components are electrophoresed according to their charge, The electrical mobility is different due to the molecular size and so on. Then add the known corresponding monovalent antiserum (containing anti-κ, λ light chain and various heavy chain antiserum respectively) on it. When the antibody binds to the monoclonal Ig in its zone, an antigen-antibody complex is formed and precipitated. (fixed) down. After rinsing and staining, a thick and narrow coloring zone appears, and the light chain and heavy chain type of the monoclonal Ig can be judged.
[0107] 2. Specimen collection: 35 cases of M-proteinemia samples were collected and 1...
Embodiment 2
[0123] Example 2 Identification of immunofixation electrophoresis images by computer
[0124] The authors and clinical immunologists selected 35 cases of M-proteinemia sample collection and 15 cases of immunofixation electrophoresis for publication (see literature: Song Jianqing. Clinical application of electrophoresis technology—electrophoretic atlas [M]. Version 1. Liaoning: Science Press, 2006) were provided by the Laboratory of the First People's Hospital Affiliated to Shanghai Jiao Tong University. The selection principle is not only satisfied with the M protein types of routine inspection, but also including rare types and special images on the atlas of the book. After scanning into pictures, the pictures are converted into data with the button wizard computer software. Intelligent identification of immunofixation electrophoresis M protein components by computer programming Through the data analysis of 50 immunofixation electrophoresis images and serum protein electropho...
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