Method for identifying self-sterile S-gene type SSR molecular marker of sweet cherry variety

Abstract

The invention provides a method for identifying a self-sterile S-gene type SSR molecular marker of a sweet cherry variety. The method uses PTCR4SSR to mark, wherein the SSR primer sequence is upstream primer 5'-CATAGGTTCAAACCATACCCGTG-3' and downstream primer 5'-CTCATCTTTGTAGGGTATAATACC-3' to amplify the DNA of sweet cherry of different varieties (systems), if the amplified fragment of 255bp can be amplified, pollen S-gene exists, and a electrophoretogram can be obtained by agar gel electrophoresis; according to the agarose elelctrophoretogram, the self-sterile gene type of each variety (system) can be determined, wherein the same bands are the same S-gene type, otherwise being different S-gene types. The SAM method is used to develop SSR marked PTCR4 in sweet cherry for identifying the S-gene type of different sweet cherry self-sterile gene type varieties (systems) and guiding selection of breeding parents and configuration of pollination varieties thereof, so that the detection efficiency of the self-sterile gene type can be improved, the breeding process of the sweet cherry is shortened, and the production cost is saved.

Description

1. Technical field [0001] The invention relates to a SSR molecular marker identification method for self-incompatibility S genotype of sweet cherry varieties (lines), in particular to a method suitable for different sweet cherry self-incompatibility (S genotype) varieties (lines) SSR molecular marker identification method for identification, selection of breeding parents and allocation of pollination varieties. 2. Background technology [0002] Sweet cherry (Prunus avium L.) is a gametophytic self-incompatibility (GSI), genetically controlled by a single site or locus (called S locus) with multiple alleles on the chromosome , at one S locus, a plant population may contain multiple S alleles (S alleles), which are called S genes. Most of the sweet cherry varieties are self-incompatible varieties, and pollination trees or artificial assisted pollination must be arranged in production to obtain the desired yield and fruit quality; therefore, the SSR of the self-incompatible S ...

AI Technical Summary

Problems solved by technology

However, in terms of existing identification methods, it is mainly determined by the mutual pollination and fruit setting rate between field varieties. Although it is intuitive and effective, it has a large workload and a long cycle; although biotechnology methods have the advantages of labor saving and quickness, However, corresponding equipment and technical conditions are required, and there are certain limitations, so that the identification of the S genotype of sweet cherry varieties progresses slowly.

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