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Method for rapidly stabilizing rice backcross introgression population trait

A technology of population and traits, which is applied in the field of rapid stability of rice backcross introduction population traits, can solve the problems of time-consuming, labor-intensive, limited, difficult to apply indica rice breeding, etc., to speed up the stabilization speed, shorten the breeding cycle, and increase the frequency of homozygosity Effect

Inactive Publication Date: 2016-05-11
SHENZHEN INST OF BIOLOGICAL BREEDING & INNOVATION CHINESE ACADEMY OF AGRI SCI +1
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Problems solved by technology

At present, flower culture and distant hybridization chromosome elimination technology are two commonly used fast and stable technologies, but they are still time-consuming and laborious
The former is difficult to apply to indica rice breeding due to factors such as green seedling differentiation rate; the latter is difficult to eliminate the chromosomes where exogenous non-target genes are located by distant hybridization, so these two technologies are still in practice in rice breeding. subject to certain restrictions

Method used

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  • Method for rapidly stabilizing rice backcross introgression population trait
  • Method for rapidly stabilizing rice backcross introgression population trait
  • Method for rapidly stabilizing rice backcross introgression population trait

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Experimental program
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Effect test

Embodiment 1

[0023] 1) Material construction

[0024] Using Huanghuazhan, a high-yielding, high-quality, and widely adaptable variety widely promoted in China, and rice varieties from 4 different countries as donors, the hybrid F 1 generation, and then reincarnate the parent and F 1 Backcross to get BC 1 f 1 , BC 1 f 1 8 sets of BC are obtained by self-delivery 1 f 2 Random population (Table 1).

[0025] Table 1 BC obtained from 8 Huanghuazhan BC1 populations after two rounds of screening 1 f 2 and BC 1 f 3 High-yielding, drought-resistant, salt-tolerant strain coefficient

[0026]

[0027] 2) Screening for high yield, drought resistance and salt tolerance

[0028] In the first round of trait screening, 8 BC 1 f 2 The population seeds are divided into three parts, one part is used for drought resistance screening in reproductive growth period, one part is used for seedling salt tolerance screening, and one part is used for high yield screening in normal paddy field. 780 i...

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Abstract

The invention provides a method for rapidly stabilizing rice backcross introgression population trait. The method comprises the following steps: establishing selective introgression lines; screening the selective introgression lines; performing genotype identification on the selective introgression lines; analyzing the descendant donor introgression frequency and heterozygous genotype of the selective introgression lines; and breeding new variety of the selective introgression lines, performing regional tests on the selective introgression lines with obvious target performances under different ecological conditions at home and abroad, identifying the general performance of the variety, and approving or registering the bred new variety.

Description

technical field [0001] The invention belongs to the fields of rice molecular breeding and molecular genetics, and in particular relates to a method for rapidly stabilizing traits of a rice backcross introduction population. Background technique [0002] As we all know, the breeding of new crop varieties is a process of creating genetic variation and selecting and fixing favorable target trait variation. Hybrid breeding is an effective means of creating new variations and cultivating new varieties. It is the most widely used and most effective breeding method among various breeding methods at home and abroad. At present, most of the three-line or two-line parents of conventional varieties and hybrid rice that are popularized in my country are produced through sexual hybridization. However, the stability of hybrid offspring between rice varieties generally takes 5 to 6 generations, and the stability of hybrid offspring between subspecies is longer. Therefore, it is one of th...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 黎志康徐建龙郑天清张帆陈凯密雪飞朱亚军孙勇
Owner SHENZHEN INST OF BIOLOGICAL BREEDING & INNOVATION CHINESE ACADEMY OF AGRI SCI
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