Haploid breeding method of dendrobium candidum

A technology of Dendrobium officinale and Dendrobium candidum, applied in the field of haploid breeding of Dendrobium officinale, can solve the problems of difficulty in guaranteeing the medicinal quality of Dendrobium officinale, hindering the sustainable development of Dendrobium candidum, resistance of varieties to diseases, poor resistance to retrograde, etc. and the development of high-quality varieties, huge potential commercial value, and the effect of improving the survival rate of embryos

Inactive Publication Date: 2014-08-13
HANGZHOU QIANDAOHU LVSHANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its varieties have poor disease resistance and retrograde resistance, and are easily attacked by diseases.
After the onset, pesticides are used to prevent and control the disease. The medicinal value of Dendrobium candidum produced in this way is low, and there are even pesticide residues, which are far from being compared with wild Dendrobium candidum.
It is difficult to guarantee the medicinal quality of Dendrobium officinale
Seriously hindered the sustainable development of Dendrobium industry, the preservation of wild strains of Dendrobium and the development of high-quality varieties are imminent

Method used

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  • Haploid breeding method of dendrobium candidum

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Embodiment 1

[0029] A haploid breeding method of dendrobium officinale, comprising the steps of:

[0030] a. Get the flowers of Dendrobium officinale that have bloomed for 3-6 days, inoculate pollen at normal temperature before cultivating, and treat them in an anther callus induction medium at a low temperature of 4°C for 48 hours, and then carry out conventional culture;

[0031] b. Sterilize with a 5.6% NaCIO solution for 15 minutes, rinse with sterile water for 5 times, peel off the anthers from the sterilized flower buds in a sterile environment, try to prevent the anthers from being damaged, and inoculate them in the anther callus induction culture Base, seal with plastic wrap;

[0032] c. Carry out dark constant temperature culture at 25°C for 2-3 days, and then carry out dark shaking culture for 12-15 days to obtain embryoid bodies;

[0033] d. Collect the embryoid body, inoculate it into the differentiation medium, and then cultivate it in the tissue culture room under light for ...

Embodiment 2

[0039] A haploid breeding method of dendrobium officinale, comprising the steps of:

[0040] a. Get the flowers of Dendrobium officinale that have bloomed for 3-6 days, inoculate pollen at normal temperature before cultivating, and treat them in an anther callus induction medium at a low temperature of 4°C for 48 hours, and then carry out conventional culture;

[0041] b. Sterilize with a 5.6% NaCIO solution for 15 minutes, rinse with sterile water for 5 times, peel off the anthers from the sterilized flower buds in a sterile environment, try to prevent the anthers from being damaged, and inoculate them in the anther callus induction culture Base, seal with plastic wrap;

[0042] c. Carry out dark constant temperature culture at 25°C for 2-3 days, and then carry out dark shaking culture for 12-15 days to obtain embryoid bodies;

[0043]d. Collect the embryoid body, inoculate it into the differentiation medium, and then cultivate it in the tissue culture room under light for 1...

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Abstract

The invention discloses a haploid breeding method of dendrobium candidum, which comprises the following steps: a. taking dendrobium candidum flowers blooming for three to six days, inoculating pollen at normal temperature, treating for 45-50 hours at 4-DEG C low temperature in anther callus inducement culture medium, and then carrying out conventional culture; b. sterilizing for 15 minutes with NaCIO solution, peeling out anther from the sterilized flower buds under sterile environment, inoculating into the anther callus inducement culture medium, and sealing with a preservative film; c. carrying out dark constant-temperature culture for 2-3 days at 25 DEG C, then carrying out dark shaking culture for 12-15 days to obtain an embryoid; d. collecting the embryoid, inoculating to a differential medium, to obtain haploid plant; and e. conducting doubling germination on the root of the haploid plant with colchicine. According to the haploid breeding method, the problems of storing the wild dendrobium candidum strain and developing high-quality strains can be well solved; the variety of the dendrobium candidum can be improved, and the new variety of dendrobium candidum can be bred.

Description

technical field [0001] The invention belongs to the field of haploid breeding, in particular to a haploid breeding method of dendrobium officinale. Background technique [0002] Dendrobium candidum is a commonly used traditional Chinese medicine in my country, which has unique effects such as nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat. In the medical classic "Tao Cang" of the Tang Dynasty, Dendrobium candidum was listed as "the first of the nine Chinese immortal herbs", and it has been listed as a royal tribute since the Tang and Song Dynasties. In recent years, a large number of in-depth research and development have found that Dendrobium candidum also has anti-tumor, anti-aging, enhancing the body's immunity, dilating blood vessels and anti-platelet aggregation. Therefore, people's demand for Dendrobium officinale has greatly increased. Due to people's long-term unrestricted, predatory and destructive digging, and the low seed s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 孙从正李振达
Owner HANGZHOU QIANDAOHU LVSHANG BIOTECH
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