Type H5 and H9 avian influenza virus and multiple detection method for newcastle disease virus

Abstract

The invention discloses a method of detecting whether the birds and the related products are infected with H5 type or H9 type bird-flu virus or Newcastle disease virus. In the method of the invention, the trachea, cloacal swabs, blood, dung, or organs and tissues of dead birds are sterilely taken, and then milled into milk suspension by sterile physiological saline; the genomic RNA are extracted, and the RNA is used as template; the H5A, H9A and F are used as the primer for the targeted gene to undergo RT-PCR; the product obtained is electrophoresed; whether the birds detected are infected with H5, H9 type bird-flu virus or Newcastle disease virus are analyzed according to the electrophoretogram.

Description

technical field [0001] The invention relates to a method for detecting whether poultry and its products are infected with infectious diseases, specifically a method for detecting whether poultry and its products are infected with H5 or H9 avian influenza virus or Newcastle disease virus. Background technique [0002] Avian influenza virus (AIV) and Newcastle disease virus (NDV) have been the most important pathogens affecting the poultry industry. Although the prevalence of avian influenza and Newcastle disease has been controlled through the widespread use of vaccines, in recent years, the H5 and H9 types of avian influenza and Newcastle disease that are prevalent in my country have shown atypical symptoms in highly immune flocks, often with the production of Significant drop in egg production, low mortality rate, respiratory symptoms and later neurological symptoms are the characteristics. It is difficult to distinguish the subtype of avian influenza virus from Newcastle di...

AI Technical Summary

Problems solved by technology

Although these technical methods have been recognized, adopted and applied, some of them have low sensitivity, some have low specificity, some have low detection rates, some have a long detection period, and some have too much cost, and these Most of the methods need to use live viruses, and there is a hidden danger of spreading the poison to the surrounding environment

There are many difficulties in carrying out this work in general grassroots units, especially the pollution of the inspected materials, which makes it more difficult to detect

Therefore, the isolation and identification of Newcastle disease virus requires not only strict aseptic sampling, but also low-temperature storage and rapid inspection, which is often difficult to do in general work sites

Images