10 results about "Mesophyll Cell" patented technology

The application relates to the technical field of plant breeding, and discloses a cultivation method of a powdery mildew-resistant plant and application of a mesophyll-specific expression vector, which comprises the following steps: transforming the mesophyll-specific expression vector into the powdery mildew-resistant plant, so that the mesophyll cells in the powdery mildew-resistant plant can specifically express a powdery mildew-resistant gene MLO; and the powdery mildew-resistant plant is obtained by making the powdery mildew-resistant gene MLO in the plant be mutated. According to the application, the mesophyll-specific expression vector is introduced into the powdery mildew-resistant plant (the plant has a premature aging phenomenon) with the function of the powdery mildew-resistant gene MLO being inactivated, so that the mesophyll cells in the plant can specifically express the powdery mildew-resistant gene MLO, and the plant which is resistant to powdery mildew and has no growth defects is obtained. The cultivation method provided by the application can eliminate the premature aging phenotype of the existing powdery mildew-resistant plant with premature aging, successfully decouples the mlo-mediated disease resistance and growth demand, and provides a new perspective for further analyzing the MLO-mediated disease mechanism research.

This invention relates to the field of crop cultivation technology and discloses a high-temperature-resistant cultivation technique for mung beans. The technique includes immersing mung bean seeds in a calcium chloride aqueous solution in the dark, then removing them and allowing them to air dry naturally until the seed coat is free of visible water before sowing them in the field. During the seedling to initial flowering stage, the relative soil moisture content is controlled. During the initial flowering to pod-setting stage, when a weather warning indicates continuous high temperatures, a metastable silicic acid and proline composite sol is sprayed onto the plant canopy leaves. The composite sol is prepared by protonating potassium silicate in an acidic substrate containing ascorbic acid, thus halting the polymerization process. Pre-sowing seed soaking promotes calcium ion absorption and initiates stress-resistance signal transduction, improving the seedling's basic heat resistance. Spraying the sol utilizes the dehydration and condensation of silicic acid to form an inorganic network covering layer, limiting water loss. Combined with proline penetration into mesophyll cells for osmotic regulation, this maintains leaf moisture content and ensures a certain number of pods.

The invention discloses a tobacco leaf curing method based on chloroplast disintegration induced by a low-frequency electric field, and relates to the technical field of tobacco leaf processing, and the scheme is as follows: fresh tobacco is pretreated, yellow tobacco leaves with mesophyll cells having activity are harvested, and the tobacco leaves are bundled and then hung in a curing barn; an electric field is prepared before baking, a low-frequency electric field is arranged in a baking room, and the intensity of the electric field is stably controlled to be 0.2-0.5 V / cm; in the yellowing stage, the tobacco leaves are continuously placed in the electric field, and the electric field intensity, temperature and humidity of the curing barn are adjusted according to the early, middle and later yellowing stages to achieve balanced yellowing; a color fixing stage: completely closing the electric field and increasing the temperature of the curing barn to fix the color of tobacco leaves; in the stem drying stage, the temperature is further increased, the humidity is reduced, and the tobacco leaves are thoroughly dried and maintain toughness; in the tobacco leaf moisture regaining stage, cooling and improving humidity to soften the tobacco leaves. Through combination of a low-frequency electric field and temperature and humidity adjustment, the tobacco leaf yellowing time is shortened, meanwhile, color brightness and chemical component coordination are kept, and the problems that traditional yellowing is long in period, prone to browning and the like are solved.

The invention relates to a group of N-glycosylation mutants of type I alpha-mannosidase and application of the N-glycosylation mutants. According to the invention, N-glycosylation modification of tomato-sourced I-type alpha-mannosidase SlMNSI1 at amino acid residues N288 and N334 is analyzed for the first time, and the modification sites are located in a conserved structural domain of a GH47 glycoside hydrolase family. N288A, N334A and N288A / N334A mutants are further constructed, and the N288A, N334A and N288A / N334A mutants are transiently expressed in mesophyll cells of the nicotiana benthamiana. Positioning analysis shows that the mutant can still be partially positioned in the Golgi apparatus, but protein aggregates appear and are transferred to the periphery of the endoplasmic reticulum. Protein stability detection shows that the N-glycosylation modified mutant is obviously accelerated to degrade after being treated by the cycloheximide, which indicates that the glycosylation modification of the N288 and N334 sites has an important effect on the stability and subcellular localization of the SlMNSI1. The functional characteristics of key glycosylation sites of the type I alpha-mannosidase are disclosed for the first time, a group of N-glycosylation mutants with structure and function differences are provided, and the N-glycosylation mutants can be used as important tools for researching plant glycosylation regulation, protein engineering modification and gene function identification and have wide application prospects.

The application provides a molecular marker for detecting a premature senescence gene of crops and application thereof, and belongs to the technical field of crop breeding. The molecular marker for detecting the premature senescence gene of crops can detect the deletion of four bases AACA in the 11th exon of the coding region of the gene LOC_Os03g31550, so as to form a premature senescence related gene caused by frame shift mutation. Research shows that the rice containing the premature senescence related gene has a stable premature senescence phenotype, the leaf tips of the second leaf and the third leaf are dried and the premature senescence phenotype appears on the 10th day after sowing in a field environment, and the aging lasts to the mature period. The photosynthetic pigment content of mesophyll cells is obviously reduced in the tillering period, the chloroplast structure is changed, the light damage is serious, the photosynthesis is weakened, and the uric acid content is reduced. The application provides a molecular basis for further in-depth research on gene regulation of aging, and provides a reference for cultivating new varieties resistant to premature senescence by using the premature senescence gene.

The application discloses a method for increasing drought adaptability of plant osmotic regulation substance accumulation, and belongs to the field of plant genetic engineering and drought-resistant molecular breeding. First, a plant to be transformed is combined with 13C-glutamic acid and 15N-choline double isotope labels to quantize carbon and nitrogen flow redistribution of proline synthesis pathways and glycine betaine synthesis pathways in leaf cells and vascular bundle companion cells, and to identify rate-limiting enzyme nodes. Second, a multi-cistronic plant expression vector is constructed. A first expression box is driven by a leaf-specific promoter and a drought response cis-element composite promoter to drive P5CS. A second expression box is driven by a companion cell-specific promoter and a drought response cis-element composite promoter to drive BADH. Positive transformants are obtained through Agrobacterium mediation or gene gun transformation and molecular screening. The expression quantity of the positive transformants is increased by 5 to 50 times under stress and falls back to a baseline within 72 hours of rehydration. Root activity is significantly improved, and yield is preserved.

The present application relates to BnaWRKY25 gene and its application in regulating the growth and development of Brassica napus seedling stage. A plurality of BnaWRKY25 genes are isolated and identified, and the expression of the BnaWRKY25 gene is regulated through gene overexpression technology and CRISPR / Cas9 gene editing technology, and it is found that the BnaWRKY25 gene positively regulates the root length, leaf size and mesophyll cell number of the oilseed rape seedling stage, and can be used for promoting the growth of the oilseed rape seedling stage, laying a foundation for the yield and quality improvement of the oilseed rape, providing a new germplasm resource for the breeding of the oilseed rape, greatly improving the breeding efficiency, and accelerating the breeding process.

The application discloses a flexible wearable sensor for detecting net photosynthetic rate of plants and a preparation method and application thereof, and the sensor is composed of a fixing layer, a substrate layer, an electrode layer and a conductive layer, and stretchable net structures are formed at both ends of the substrate layer; the electrode layer is a concentric circle sensing electrode, so that a uniform radial alternating current field is generated, and the conductive layer is closely attached to the surface of the leaf to reduce the interface contact impedance. The detection system constructed by the sensor can effectively solve the technical problem that the net photosynthetic rate of plants cannot be monitored in a long-term, non-destructive, real-time and in-situ manner in the prior art according to the high correlation between the alternating impedance parameters of the plant leaves under a specific characteristic frequency and the ion exchange of the leaf cells, by in-situ collection of the impedance change of the leaves, and by combination of environmental parameters, to calculate the net photosynthetic rate.

The invention belongs to the technical field of crop stress resistance, and particularly relates to a zinc-based oxide nano-composite particle and application thereof in improving the salt tolerance of plants. The zinc-based oxide nano-composite particles are obtained by wrapping zinc-based oxide nano-particles with a water-soluble high-molecular polymer. The zinc-based oxide nano-composite particles can maintain the stable state of active oxygen in mesophyll cells under salt stress, improve the chlorophyll content of cotton leaves, finally improve the photosynthetic ability of cotton and remarkably promote the growth of cotton seedlings. The zinc-based oxide nano-composite particles are green and safe, and have the potential of becoming a nano regulator to promote the growth of cotton in saline-alkali soil.

This invention discloses a plant growth regulator for controlling nitrogen fixation and stress resistance in plants and its application, belonging to the field of agricultural biotechnology. The plant growth regulator uses molybdenum disulfide nanosheets as the active component, which are dispersed in an aqueous solution to prepare a spray solution. A surfactant is added to enhance leaf wettability, and the solution is applied to soybean plants during their growth period via foliar spraying. The molybdenum disulfide nanosheets are internalized into mesophyll cells through open stomata in the leaves, and further undergo biotransformation to form inorganic molybdenum (Mo(VI)) and organic molybdenum (Mo-RCOOH), which are then transported to the roots and root nodules. By delaying root nodule senescence, upregulating the expression of the soybean hemoglobin gene, and prolonging the active nitrogen fixation period, the symbiotic nitrogen fixation efficiency of soybeans is significantly improved. Under adverse conditions such as nitrogen limitation and salt stress, this physiological process significantly improves the overall growth status of soybean plants.