43 results about "Mesophyll Cell" patented technology

The invention refers to a particular folium mori black tea and the processing method, adopting fresh ripe folium mori to make it by fading, knead-cutting, fermenting and drying. The processing methodincludes: placing the washed-out fresh folium mori in the fading tank for fading for 2-4hours at 20-60íµ; placing the faded folium mori in knocker and rotor machine for knocking and rubbing to make the mesophyll cell fully broken and form particle shape; and then fermenting the particular folium mori; then fully drying the fermented folium mori. It has functions of dredging wind and clearing heat, clearing liver and brightening eye, relieving cough and asthma, and smoothing lung and sobering up.

The invention belongs to the technical field of processing of wormwood, and particularly discloses a production and processing technology of moxa floss. The production and processing technology of moxa floss comprises the following steps: (1) material selection, (2) processing of folium artemisiae argyi, (3) crushing, (4) sieving, (5) pulverizing, (6) coarse sieving, and (7) fine selecting. Compared with the prior art, the production and processing technology has the characteristics that the folium artemisiae argyi is humidified and air-dried before being crushed, mesophyll cells and moxa floss fibers of the folium artemisiae argyi can be conveniently separated effectively, therefore, the making process of the moxa floss is rapid, and the quality of the obtained moxa floss is good. A self-made sieving device is used in the process, and time and labor are saved in the making process of the moxa floss.

The invention relates to a method for observing the epidermal and internal microstructures of a leaf by using a transparent leaf, which has low cost and can accurately observe the microstructures of leaf. The technical scheme in the method comprises the following steps: firstly, processing the leaf, and cleaning and soaking the leaf in ethanol to remove chlorophyll; then soaking in alkaline solution to break the mesophyll cells; decolorizing in sodium hypochlorite; rinsing with flowing water; placing in hydrated chloric acid; taking out the leaf; rinsing with flowing water; dewatering with alcohol solutions with gradient concentrations; placing in xylene solution to enable the leaf to be transparent; and sealing with neutral balsam or water; and observing and picking images with optical microscope to observe the internal and external structure at different levels of the leaf. The method has the advantages of less material selection, low cost, less work load, high success rate and good effect, and is suitable for observation of various leaves.

The invention belongs to the technical field of tea beverages, and particularly relates to a making method of black tea. The making method comprises the following steps of performing sorting, performing withering, performing rolling, performing fermentation, and performing drying. Through special withering treatment, the breakage rate of cells of tea leaves is increased, so that more substances in mesophyll cells during fermentation can be oxidized, accumulation of beneficial components is facilitated, and beneficial components such as water extractions, amino acids, caffeine, flavone, soluble sugar, theaflavin, thearubigins, theabrownin and catechin are all increased, the nutrient value of the black tea is increased, and the mouth feel of the black tea is improved. The black tea prepared by the method disclosed by the invention is red bright in soup color, pure in fragrance, and sweet and pure in taste and has fragrance of flowers.

The invention belongs to the technical field of extraction of plant cell vacuoles and particularly relates to an extraction method of halogeton mesophyll cell vacuoles. The extraction method of the halogeton mesophyll cell vacuoles is mainly characterized by comprising the following steps: (1) taking mesophyll; (2) enzymolysis and purification of protoplast: cutting the mesophyll obtained in the step (1) from the stalk along the mesophyll base, then putting in an enzymatic hydrolysate, carrying out enzymolysis for 2.5-3.0h at the temperature of 22-25 DEG C under the dark condition, and after the enzymolysis is finished, adding isometric washing liquid into the enzymatic hydrolysate; and (3) obtainment and purification of vacuoles: adding digitonin into the protoplast obtained in the step (2) so as to realize enrichment of pure vacuoles. The digitonin is used for dissociating the plasma membrane, thereby simplifying common complex programs of density gradient centrifugation for extracting the vacuoles. According to the invention, the process of carrying out tender mesophyll protoplast enzymolysis to obtain high-purity vacuoles only takes about 4h. The method realizes simple, efficient and fast extraction of the halogeton mesophyll cell vacuoles. The vacuoles obtained by the method are relatively high in purity, more in quantity, little in impurity contamination and good in integrality.

The invention belongs to the technical field of tea drinks and in particular relates to a processing method of black tea. The processing method of the black tea comprises the following steps: sorting, withering, carrying out freeze drying, mixing, rolling, fermenting and drying. The processing method of the black tea has the beneficial effects that the prepared black tea adopts a special processing technology, so that mesophyll cells of tea leaves are ruptured in a relatively mild state, various enzymes in cytoplasm and ingredients in solution 1 are fully contacted, all kinds of biochemical reactions in foliar cells are promoted, synthesis of aroma substances is promoted, aroma of the processed black tea is greatly improved, flowery flavor is strong, tea soup is bright red, taste is sweet and mellow, the highest amino acid content in the tea soup can reach up to 3.25%, theaflavin content can reach up to 0.30%, and aqueous extract content can reach up to 40.14%.

The invention discloses a method for separating and purifying amomum villosum mesophyll protoplasts. The method comprises the following steps: preparing and pre-treating materials, separating protoplasts by an enzymolysis method, and purifying the protoplasts by combining a sedimentation method and a drifting method. By studying the factors affecting the yield and vitality of the protoplasts, suchas material pretreatment or no material pretreatment, enzyme types, enzyme concentration, enzymolysis time and concentration of an osmotic pressure regulator and by studying the influence of different purification methods on the purification effect of the mesophyll cell protoplasts, the invention optimizes the experimental conditions for the separation and purification of the amomum villosum mesophyll protoplasts so as to obtain the high-yield and high-vitality amomum villosum protoplasts, and lays a foundation for the cultivation of new varieties of amomum villosum by somatic cell fusion.

The invention discloses an efficient transient gene expression method for hybrid liriodendron taking mesophyll protoplast as a receptor. The efficient transient gene expression method for the hybrid liriodendron taking mesophyll protoplast as the receptor comprises the following steps: preparation of the mesophyll protoplast of the hybrid liriodendron, preparation of plasmids, conversion and observation. The protoplast of mesophyll cells of the hybrid liriodendron is used as a receptor system of genetic transformation, by a PEG mediated method, green fluorescent protein (GEP) genes are used as reporter genes, and efficient transient expression of GFP genes is detected through a fluorescence microscope. The leaf blade of the hybrid liriodendron is used as a source of the protoplast, materials are taken conveniently, vitality is high, and an established transient expression system can provide a rapid and convenient operation platform for research of gene functions of liriodendron tulipifera and magnoliaceae.

The invention relates to a method for increasing the resistance to pathogens that penetrate mesophyll cells in a plant, or in an organ, tissue or cell of a plant. Said method is characterised in that the callose synthase activity in the plant, or in an organ, tissue or cell of said plant is reduced in comparison to control plants.

The invention belongs to the technical field of biology, and provides a separating and extracting method for thylakoid membranes of C4 plant mesophyll cells and bundle sheath cells. Through the mechanical action of a high-speed blender, thylakoid membranes of C4 plant mesophyll cells and bundle sheath cells can be respectively obtained. The method is simple in technology and easy to operate; the extraction time can be shorter by adopting the technology to extract the thylakoid membranes; compared with an enzyme hydrolysis method, the method is simple in technology, short in extraction time andlarge in processing capacity; and the thylakoid membranes of the C4 plant mesophyll cells and bundle sheath cells can be respectively obtained through the method, so that required proteins can be obtained.

The invention discloses a method for researching photosynthesis through combination of a chlorophyll fluorescence technique and a protoplast system, and application thereof. Genes related to photosynthesis can be screened at high throughput quickly through instantly expressing or silencing one or more of genes in a protoplast, determining change of chlorophyll fluorescence of the protoplast of an arabidopsis mesophyll cell, and comparing an image thereof.

The invention discloses a diagnosis method for the level of potassium nutrients in a tomato on the basis of the change of a microstructure of a leaf, and is used for diagnosing the level of the potassium nutrients in the tomato on the basis of the microstructure of the leaf. The diagnosis method comprises the following steps: firstly, acquiring a microscopic structure image and an ultramicroscopic structure image of the tomato leaf by means of a scanning electron microscope system and a transmission electron microscope system; and then extracting the sizes and the density of stoma on the surface of the leaf, the height and the density of tomenta on the surface of the leaf, the density of vascular bundles on the surface of the leaf, the thickness of sponge tissues and the thickness of palisade tissues on the section of the leaf and the thickness of the whole leaf as well as the characteristics of an ultramicroscopic structure of mesophyll cells under the conditions of different potassium nutrients, and carrying out representation and statistic analysis. The diagnosis method disclosed by the invention can be used for diagnosing information of the potassium nutrients in the tomato as early as possible and can be applied to detection of the level of potassium nutrients in greenhouse tomatoes.

The invention discloses a method for improving plant yield and its special expression cassette. The method of the invention comprises inducing the expression cassette capable of specifically expressing a pyrophosphoric acid hydrolase in plant mesophyll cells into plants, screening and obtaining plants capable of expressing the pyrophosphoric acid hydrolase, that is the plant with improved yield. The expression cassette capable of specifically expressing the pyrophosphoric acid hydrolase in plant mesophyll cells comprises a series cyFBPase promtor, a pyrophosphoric acid hydrolase gene and an OCS terminator in sequence. The method of the invention can greatly improve the yield level of the transgene plants, especially the transgene crops.

The present invention relates to the field of tea leaf processing and particularly relates to a manufacture method of cliff top rock tea. The manufacture method comprises the following steps: picking,withering, rocking, stir-frying, rolling, drying, tea roasting and sealed packaging. The picking is specifically as follows: tea leaves are picked; the picked tea leaves are placed in a breathable bamboo basket; the thickness of the tea leaves in the bamboo basket is between one-half and two-thirds of the depth of the bamboo basket; at the same time, the bamboo basket is shaken for 30 minutes; the tea roasting is specifically as follows: the dried obtained tea leaves are roasted three times with each roasting temperature of 80 DEG C-90 DEG C and for each roasting time of 7-9 hours; during theroasting, the tea leaves are stirred once every half hour; and after each roasting, the tea leaves are sealed and put still for 28-31 days. The picked tea leaves are placed in the bamboo basket for shaking; water and soluble substances in stalk stems are together promoted to be transported to mesophyll cell tissues through transfusion tissues; the tea roasting can remove water, maintains quality,increases liquid color, improves taste and mellowness, and promotes ripening of tea fragrance.

The invention constructs a FoMV-mediated GFP-ATG8 expression vector and application thereof. A method takes TaATG8a as a target for monitoring autophagy, a VOX technology based on FoMV is adopted forexpressing GFP-TaATG8a fusion protein on a wheat plant, the expression situation and subcellular localization situation of the fusion protein in leaves and root tissues are observed through fluorescence, and a technical platform for monitoring the autophagy level on a wheat living plant with overexpression GFPTaATG8 as the target is established. The platform is successfully applied to observationof representation autophagosomes and autophagovesicles in leaf epidermis, mesophyll cells and root cells under hunger and drug treatment, and the invention shows that the vector can be applied to autophagy level monitoring and autophagy function research in the wheat growth and development process or the environmental factor response process. Research results provide technical support for deep exploration of autophagy regulation and control mechanisms and physiological functions of important crop wheat.

The invention relates to a complex enzyme preparation for a leaching tobacco material and an enzyme leaching method, and belongs to the technical field of regenerated tobacco processing. The active component of the complex enzyme preparation comprises 0.1-1000U of lysozyme, 1-1000 U of cellulase, 0.1-1000U of alpha-amylase, 0.1-1000 U of proteolytic enzyme and 0.1-1000U of pectinase, wherein the amount is the adding amount in per gram of tobacco material. The temperature in leaching is controlled at 30-60 DEG C, and the liquor ratio is 1:5-10; the enzyme preparation is added to be stirred for 1-4 hours; and after solid-liquid separation, solid phase is taken and water is further added according to original solid weight for circularly extraction for 1-2 times. According to the invention, mesophyll cell and cabo fiber structures, composition, physical properties and the like of tobacco are adequately considered, combined wall breaking effects of lysozyme, pectinase and cellulase are introduced, and enzyme catalytic action of proteolytic enzyme, alpha-amylase and cellulase for degrading biomacromolecule is considered, so that the compact structure of tobacco and straw is dredged, leach of inclusions of materials is increased, the dissolution rate is enhanced and the quality of the extracting liquid is improved.

The invention provides a method for detecting the effectiveness of intracellular moisture of plant leaves, and belongs to the technical field of water-saving irrigation and crop information detection.Firstly, subjecting plant leaves to be detected to water saturation and drying treatment; measuring the relative water content, the leaf thickness and the mesophyll cell thickness of the leaf at eachwater loss moment; and calculating the percentage of the relative water content, the leaf thickness and the mesophyll cell thickness of the leaf at each water loss moment in each value of the previous adjacent water loss moment, and further obtaining the relative variation of the relative water content, the leaf thickness and the mesophyll cell thickness of the leaf at each adjacent water loss moment, thereby judging the effectiveness of the intracellular water of the plant leaf. According to the method, the moisture condition of the leaves can be evaluated in time, a basis is provided for advanced prediction of water-saving irrigation crop water-requiring nodes, and the test process is not limited by surrounding environmental factors.

The invention relates to a method for observing the epidermal and internal microstructures of a leaf by using a transparent leaf, which has low cost and can accurately observe the microstructures of leaf. The technical scheme in the method comprises the following steps: firstly, processing the leaf, and cleaning and soaking the leaf in ethanol to remove chlorophyll; then soaking in alkaline solution to break the mesophyll cells; decolorizing in sodium hypochlorite; rinsing with flowing water; placing in hydrated chloric acid; taking out the leaf; rinsing with flowing water; dewatering with alcohol solutions with gradient concentrations; placing in xylene solution to enable the leaf to be transparent; and sealing with neutral balsam or water; and observing and picking images with optical microscope to observe the internal and external structure at different levels of the leaf. The method has the advantages of less material selection, low cost, less work load, high success rate and goodeffect, and is suitable for observation of various leaves.

The invention discloses application of a chlorosis seedling lethality 1 (CSL1) gene to regulation of development of rice chloroplasts. According to the invention, a lethal rice mutant at an etiolatedseedling stage is isolated from a rice T-DNA mutant library; the lethal rice mutant is inserted into a seventh intron of the CSL1 gene by means of T-DNA insertion; a 109bp sequence is deleted at a T-DNA insertion position; the chlorophyll content of the CSL1 mutant decreases; single-layer or multi-layer autophagosomes appear in the mesophyll cells; starch grains in the chloroplasts increase; a thylakoid structure is disordered; and the expression quantity of chloroplast-related genes is changed. meanwhile, the invention further constructs a knockout plant (the CSL1 gene has a deletion of one base A at the site 46 of exon 5, or a deletion of one base A at the site 47 and substitution of the base A with the base T at the site 48) by means of gene knockout, and the knockout plant has the samephenotype as the CSL1 mutant, thus showing that the CSL1 gene is involved in regulation of development of the rice chloroplasts.

The invention discloses a method for preparing Arabidopsis thaliana mesophyll cell protoplasts. The specific steps are as follows: cut the Arabidopsis leaves of 3-5 weeks and fix the upper epidermis and the lower surface of the leaves with inelastic paper tape or cloth tape Epidermis; Gently tear off the inelastic paper tape or cloth tape on the lower epidermis of the leaves, put the cut leaves into a petri dish containing enzymatic hydrolysis solution; place the petri dish with leaves on a shaker for cultivation 30-90min or until most or all of the protoplasts are detached from the leaves; transfer the enzymolysis solution containing the protoplasts to a round-bottomed centrifuge tube, and collect the protoplasts by centrifugation; wash the protoplasts with pre-cooled W5 solution, Resuspend protoplasts with W5 solution and place on ice for a minimum of 30 minutes. The invention cultures the protoplasts under dark conditions, effectively solves the phenomenon of cell death and aggregation caused by light culture, and can greatly improve the transformation efficiency.

The invention relates to the field of plant virus inoculation, in particular to a seedling-stage injection inoculation method for zucchini cucumber mosaic virus. The method adopts a needle tube injection mode, and can be used for inoculation when zucchini plants are cultured until cotyledons are completely unfolded, compared with the existing needle tube injection method, the inoculation time is greatly advanced, and the condition of the zucchini seedlings can be investigated 10 days after inoculation. According to the method, the virus inoculation amount is easy to control, damage to leaves is small and is closer to the field morbidity situation, inoculation liquid can be directly injected into mesophyll cells, observation is easy, and missed inoculation is reduced.

The invention specifically relates to a glutathione peroxidase GhGPX8 gene with antioxidation effect in cotton and application of the same to gene engineering, belonging to the field of plant gene engineering. The gene includes 1491 bp bases and has a base sequence as shown in SEQ ID No. 1. A coding sequence of the gene includes 501 bp bases; a protein coded by the coding sequence is glutathione peroxidase GhGPX8 of cotton and contains 166 amino acids. According to the invention, a vector p1300GFP-GhGPX8 and a vector pYES2-GhGPX8 are constructed to prove the GhGPX8 is located in the cytoplasm of mesophyll cells and indicate the glutathione peroxidase GhGPX8 is capable of removing active oxygen. Overexpression of the GhGPX8 gene is applicable to cultivation of antioxidative cotton varieties and improvement of the adversity stress resistance of cotton.

The invention discloses a preparation method of a litsea cubeba mesophyll cell protoplast. The method comprises the following steps that sterile litsea cubeba leaves are obtained; the sterile litsea cubeba leaves are cut into strips and placed in enzymatic hydrolysate, and under the dark condition, standing and enzymolysis are conducted for 6-12 hours, wherein the mass-volume ratio of the strips to the enzymatic hydrolysate is (1-3):(30-50); the enzymatic hydrolysate obtained after enzymolysis is filtered, filtrate is taken and subjected to centrifugal treatment, supernatant is discarded, andthe protoplast is obtained; a protoplast washing solution A is added into the obtained protoplast, and the protoplast is resuspended; then a protoplast washing solution B is added, after centrifugation, protoplast cells at the liquid level boundary are collected, and the litsea cubeba mesophyll cell protoplast is obtained. According to the preparation method, the litsea cubeba mesophyll cell protoplast can be efficiently obtained, the obtained protoplast is normal in form and free of damage, (8.69+ / -0.57)*10<6> protoplasts can be obtained from each gram of fresh leaf tissue, and important technical support is provided for basic research and application research of the litsea cubeba.

The invention relates to a method for efficiently enriching plant mitochondria through protoplast. The technical core of the method is acquisition of the protoplast and filtration of a filter membrane, and mitochondria are efficiently separated and purified mainly aiming at plant mesophyll cells and other tissue components capable of dissociating the protoplast. The method comprises the followingsteps: 1) culturing a plant material; 2) dissociating the protoplast; 3) naturally spalling the protoplast by using osmotic pressure; 4) performing differential centrifugation to obtain crude mitochondria; 5) filtering a heavy suspension buffer of the crude mitochondria with a needle filter made of PES material to obtain pure mitochondria; and 6) detecting the purity of the mitochondria through afluorescence microscope. The method is convenient to operate, long in consumed time, stable in effect and capable of efficiently enriching the mitochondria, and has important significance in exploringthe mitochondria functions of plants.

The invention discloses a method for isolating and purifying mesophyll protoplasts of P. vulgaris, including the steps of material preparation and pretreatment, separation of protoplasts by enzymatic hydrolysis, and purification of protoplasts by combining sedimentation and drifting methods. By studying the factors affecting the protoplast yield and activity, such as whether the material is pretreated, the type and concentration of enzymes, the enzymatic hydrolysis time, the concentration of osmotic pressure regulators, and the effects of different purification methods on the purification effect of mesophyll cell protoplasts, the method optimizes the The experimental conditions for the isolation and purification of the mesophyll protoplasts of P. solani to obtain high-yield and high-vigorous protoplasts of P. vulgaris, laid the foundation for the cultivation of new varieties of P. vulgaris through somatic cell fusion.

The invention discloses a method for effectively extracting plant mitochondria. The method is characterized by combining differential centrifugation with an inorganic alumina filter membrane, mainly aims at plant mesophyll cells, efficiently separates and purifies mitochondria, and comprises the following steps: 1) culturing of a plant material; 2), preparation of a grinding buffer solution; 3), sample preparation; 4), syringe needle filter membrane filtration; and 5), fluorescence microscope mitochondrion detection. The method is convenient to operate and stable in effect, and has important significance in plant mitochondrion function exploration.

The invention relates to the field of transparent conductive films, in particular to a composite transparent conductive film based on plant veins and a preparation method thereof. The composite transparent conductive film is formed by compounding the plant veins and silver nanowires-MXene through a deposition method. The method comprises the following steps: (1) etching plant leaves with a sodium hydroxide aqueous solution, removing mesophyll cells, washing with deionized water, and drying to obtain a plant vein substrate; (2) flattening the plant vein base to obtain a flat plant vein base; (3) soaking in a silver nanowire ethanol solution, drying to obtain a silver nanowire conductive layer, soaking the veins of the obtained silver nanowire conductive layer in an MXene solution, and drying; and (4) flattening the silver nanowire-MXene conductive network veins transparent conductive film obtained in the step (3). The conductive film has the characteristics of high conductivity, high light transmittance, high adhesion, high durability, high flexibility, high mechanical stability and the like, and has a wide application prospect in electronic device.

The invention discloses a herb tea for relieving a pain. The herb tea is characterized by comprising the following raw materials in parts by weight of 10-15 parts of radix paeoniae rubra, 5-8 parts of fructus forsythiae leaves, 5-10 parts of gynostemma pentaphyllum, 5-8 parts of argy wormwood leaves, 1-3 parts of Sichuan lovage rhizomes, 1-3 parts of abrus cantoniensis and 20-30 parts of fresh tea leaves. According to the herb tea, six kinds of medicines are used, the radix paeoniae rubra, the fructus forsythiae leaves and the gynostemma pentaphyllum are sovereign medicines, the argy wormwood leaves are a minister medicine, the Sichuan lovage rhizomes are an assistant medicine, the abrus cantoniensis is an envoy medicine, the principle of sovereign, minister, assistant and envoy is used, and the six kinds of medicines cooperate with the fresh tea leaves; catechu is a main component of the fresh tea leaves, the catechu has main components of catechin and epicatechin, the epicatechin has the effects of alleviating a pain and resisting inflammation, and the release of the epicatechin from mesophyll cells is promoted; besides, beneficial substances in the six kinds of traditional Chinese medicines, such as glycoside and ketone substances, are combined, so that the functions of combined medicines for relieving a pain and alleviating a pain can be amplified, the herb tea does not contain a nervous stimulant, only effects related to the function of relieving a pain are reserved, and the problem related to addiction can be solved or restrained.

The invention refers to a particular folium mori black tea and the processing method, adopting fresh ripe folium mori to make it by fading, knead-cutting, fermenting and drying. The processing method includes: placing the washed-out fresh folium mori in the fading tank for fading for 2-4hours at 20-60 DEG C; placing the faded folium mori in knocker and rotor machine for knocking and rubbing to make the mesophyll cell fully broken and form particle shape; and then fermenting the particular folium mori; then fully drying the fermented folium mori. It has functions of dredging wind and clearing heat, clearing liver and brightening eye, relieving cough and asthma, and smoothing lung and sobering up.

The invention relates to a processing assembly line for rocking of green tea leaves and a processing method for rocking of green tea leaves of the processing assembly line. The processing assembly line comprises a vibration circulating mechanism, a dumping device, a rolling device and a bottom plate, wherein the dumping device is mounted at the upper end of the bottom plate; the vibration circulating mechanism is mounted at the upper end of the dumping device; and the rolling device is mounted at the upper end of the vibration circulating mechanism. Through the adoption of the processing assembly line disclosed by the invention, the difficult problem that conventional rocking of green tea leaves is mostly and manually operated, in the early stage, workers need to rock green tea leaves at ahigh frequency, and continuous rocking is needed, so that manual rocking of green tea leaves in the early stage is not liable to be proper, and the situation that tea leaves are dry and hard due to loss of biological activity of mesophyll cells is easy to generate, the difficult problem that in the whole course of rocking of green tea leaves, the quantity of the tea leaves in the course of processing is not high, and the workers cannot completely control the intensity of rocking of green tea leaves, so that the situation that the integral intensity of rocking of green tea leaves is too lightis liable to generate, and the situation that aromatic substances, amino acids and catechin diffuse to leaves along with water is incomplete, the difficult problem that during manual rocking of greentea leaves, the tea leaves fall out easily to cause waste, the difficult problem that after processing is completed, the workers need to dump the tea leaves to a specified position, and the difficultproblem that the course of manual rocking of green tea leaves needs much time, labor intensity is high, and efficiency is low can be solved; and the functions of automatically performing rocking of green tea leaves and automatically dumping tea leaves can be realized.