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Method for improving plant products and expression box thereof

An expression cassette, plant technology, applied in the directions of botany equipment and methods, biochemical equipment and methods, plant genetic improvement, etc. The effect of improvement and yield increase

Inactive Publication Date: 2009-06-17
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the plants of the above-mentioned transgenic plants were dwarfed, the internodes became shorter, the content of inorganic pyrophosphate decreased, and the content of soluble sugar in the old leaves was 100 times that of the wild type

Method used

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  • Method for improving plant products and expression box thereof
  • Method for improving plant products and expression box thereof
  • Method for improving plant products and expression box thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Creation and yield identification of transgenic rice expressing pyrophosphohydrolase

[0029] 1. Creation of trans-pyrohydrolase gene rice that can express pyrophosphohydrolase

[0030]1. Construction of recombinant vector (FBP:PPase vector) expressing pyrophosphohydrolase (PPase) gene

[0031] The pCambia1391Z vector containing the cyFBPase promoter (purchased from Cambia, Australia, http: / / www.cambia.org / daisy / cambia / home.html) was digested with EcoRI to obtain a fragment of 1229 bp, namely the cyFBPase promoter (from GENBANK No. It is nucleotides 37511734-37512949 at the 5' end of NC_008394.1). Insert the cyFBPase promoter fragment obtained by enzyme digestion into the EcoRI digestion site of pCAMBIA1300 to obtain a recombinant vector, which is subjected to enzyme digestion and sequencing verification. The pCAMBIA1300 (Cambia, Australia, http: / / www.cambia.org / daisy / cambia / home.html) recombinant vector was named pCA-FBP.

[0032] Using the genome of Esch...

Embodiment 2

[0063] Example 2. Creation and yield identification of transgenic indica rice 6547 expressing pyrophosphohydrolase

[0064] In order to verify that specific expression of pyrophosphohydrolase can also increase yield in different rice varieties, we also expressed FBP:PPase by Agrobacterium in indica rice 6547, and conducted a field experiment on the yield of transgenic plants.

[0065] According to the method of Example 1, the FBP:PPase vector was transformed into indica rice 6547 (Yangzhou University), and two FBP:PPase-transformed rice lines 3003 and 3010 that could express pyrophosphohydrolase were obtained. Field planting experiments were carried out in 2005. Three points were set for each experiment, and each point was repeated twice, and each field was about 133.3 square meters. Each line includes a wild-type control with a planting row spacing of 25 cm × 15 cm. After the seeds are fully mature, sampling is performed according to the method in Step 7 in Example 1 to dete...

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Abstract

The invention discloses a method for improving plant yield and its special expression cassette. The method of the invention comprises inducing the expression cassette capable of specifically expressing a pyrophosphoric acid hydrolase in plant mesophyll cells into plants, screening and obtaining plants capable of expressing the pyrophosphoric acid hydrolase, that is the plant with improved yield. The expression cassette capable of specifically expressing the pyrophosphoric acid hydrolase in plant mesophyll cells comprises a series cyFBPase promtor, a pyrophosphoric acid hydrolase gene and an OCS terminator in sequence. The method of the invention can greatly improve the yield level of the transgene plants, especially the transgene crops.

Description

technical field [0001] The present invention relates to a method for improving plant yield and a special expression cassette thereof. Background technique [0002] Green plants make organic matter through photosynthesis, the process of plant photosynthesis to fix CO 2 Part of the produced primary photoassimilates is converted into starch in the chloroplast, and the other part is transported to the cytoplasm in the form of three-carbon sugars for sucrose synthesis and other metabolic reactions. [0003] The efficiency of photosynthesis and the distribution of its two primary assimilation products, starch and sucrose, have a great impact on crop yields. At present, increasing crop yield is mainly operated from three aspects: (1) increasing the synthesis of sucrose in the source leaves; (2) increasing the transport capacity of sucrose, that is, increasing the expression or enzymatic activity of the H+ / sucrose transporter; (3) ) to improve library utilization. [0004] On the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/55A01H1/00
CPCC12N15/8245
Inventor 储成才司丽珍张亚芳童红宁王义琴潘学彪姚海根默罕默德左建儒宋德林
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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