Method for observing epidermal and internal microstructures of leaf by using transparent leaf
A technology of microstructure and leaf epidermis, which is applied in the preparation of test samples, measuring devices, and material analysis by optical means, etc., can solve the problems of positioning observation, wrong conclusions, and cumbersome observation experiments, etc. High success rate and good effect
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[0006] According to above technical scheme, when the present invention is concretely implemented, it is realized by the following steps:
[0007] 1. Leaf treatment: fresh leaves (that is, leaves just picked from the plant) or dry leaves, wash the dust on the surface with clean water, and observe the inside of the thick leaves, after cleaning the fresh leaves, you can flatten the leaves with bare hands in advance. Cut into two pieces, and dry leaves can be soaked in water and then cut open, and then processed. For large leaves, they can be cut into small pieces, such as 1-3 square centimeters. The purpose is to remove the midrib and prevent the leaves from being too thick, so that Put it into a high-magnification microscope to observe the micromorphology. For the observation of leaf veins, the entire leaf can be directly processed;
[0008] 2. Ethanol (alcohol) immersion, be 95% ethanol immersion 46-50 hour with volume concentration, preferably 48 hours, make blade be light ye...
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