The invention discloses heat-resistant
glucose dehydrogenase and application thereof in an
enzyme fuel
cell, and belongs to the crossing field of
bioelectrochemistry and
new energy technologies. According to the invention, firstly, a heat-resistant
glucose dehydrogenase TeGDH is screened, and the heat-resistant
glucose dehydrogenase TeGDH is subjected to
heterologous expression in
escherichia coli BL21 (DE3) and shows good
heat stability and catalytic activity; teGDH is dissolved in an anolyte containing an
electron mediator anthraquinone-2, 6-disulfonic acid disodium salt, and the heat-resistant high-energy
enzyme fuel
cell is prepared by adopting a non-immobilized
system; the
maximum power density of the battery at 50 DEG C reaches 1.74 mW / cm, and the battery has good
thermal stability and long-term operation performance; the invention combines
big data mining,
enzyme engineering and electrochemical technologies, provides a novel enzyme fuel
cell solution with
high activity, high stability and low cost, and has a good application prospect.