The invention discloses a preparation method of a Co-MOF-GO film for microbial fuel cell and MFCs constructed by the preparation method, and belongs to the technical field of microbial fuel cells. Theinvention aims at solving the problems that an existing MFC anode is high in potential, low in power, short in period and the like. The preparation method comprises the following steps: 1, adding GOto water, performing uniform mixing, sequentially adding cobaltous nitrate hexahydrate and dimethyl imidazole, performing magnetic stirring, performing centrifuging, performing cleaning, and carryingout vacuum drying to obtain nanoparticles; 2, carrying out heat preservation heating under inert gas, and performing cooling to room temperature; 3, adding isopropyl alcohol and Nafion solution, and enabling the solution to uniformly disperse; 4, coating two sides of the carbon cloth with the mixture, and naturally drying the mixture. The constructed microbial electrolytic cell is an H-type double-chamber MECs. The maximum voltage of the MFCs is 0.56 V, the period is long, the COD removal rate reaches 79.195% +/-3.4766%, the coulombic efficiency reaches 9.3151% +/-0.5416%, and the maximum power density is 2303mV/m<2>.