Method for observing epidermal and internal microstructures of leaf by using transparent leaf

A technology of microstructure and leaf epidermis, which is used in the preparation of test samples, measurement devices, and material analysis by optical means, which can solve the problems of wrong conclusions, positioning observation, and cumbersome observation experiments, and achieves a high success rate. Small workload, good effect

Inactive Publication Date: 2011-06-15
HENAN UNIV OF CHINESE MEDICINE
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Problems solved by technology

However, taking a comprehensive look at the various observation methods of leaf epidermis and leaf interior using optical microscopes at home and abroad, there are certain defects, and there is no universally applicable method.
Especially for the observation of the epidermis of young leaves and papery leaves, it is difficult to separate the epidermis; for the observation of the epidermis with complex hairs, it is difficult to see the layered hairs and stomata clearly even with a scanning electron microscope, and for the internal structure of the leaves. Observation, the use of slices to observe the experiment is cumbersome, and som

Method used

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Embodiment Construction

[0006] According to above technical scheme, when the present invention is concretely implemented, it is realized by the following steps:

[0007] 1. Leaf treatment: fresh leaves (that is, leaves just picked from the plant) or dry leaves, wash the dust on the surface with clean water, and observe the inside of the thick leaves, after cleaning the fresh leaves, you can flatten the leaves with bare hands in advance. Cut into two pieces, and dry leaves can be soaked in water and then cut open, and then processed. For large leaves, they can be cut into small pieces, such as 1-3 square centimeters. The purpose is to remove the midrib and prevent the leaves from being too thick, so that Put it into a high-magnification microscope to observe the micromorphology. For the observation of leaf veins, the entire leaf can be directly processed;

[0008] 2. Ethanol (alcohol) immersion, be 95% ethanol immersion 46-50 hour with volume concentration, preferably 48 hours, make blade be light ye...

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Abstract

The invention relates to a method for observing the epidermal and internal microstructures of a leaf by using a transparent leaf, which has low cost and can accurately observe the microstructures of leaf. The technical scheme in the method comprises the following steps: firstly, processing the leaf, and cleaning and soaking the leaf in ethanol to remove chlorophyll; then soaking in alkaline solution to break the mesophyll cells; decolorizing in sodium hypochlorite; rinsing with flowing water; placing in hydrated chloric acid; taking out the leaf; rinsing with flowing water; dewatering with alcohol solutions with gradient concentrations; placing in xylene solution to enable the leaf to be transparent; and sealing with neutral balsam or water; and observing and picking images with optical microscope to observe the internal and external structure at different levels of the leaf. The method has the advantages of less material selection, low cost, less work load, high success rate and good effect, and is suitable for observation of various leaves.

Description

1. Technical field [0001] The invention relates to a method for observing the microstructure of leaf epidermis and leaf interior by using transparent leaves. 2. Background technology [0002] Morphology and anatomy research is the basic research in botany research, especially the study of leaf epidermis and leaf internal structure has important value for plant classification, plant ecology, plant physiology research and identification of leaf medicinal materials, which cannot be done by other methods. Replacement. However, taking a comprehensive look at the multiple observation methods of leaf epidermis and leaf interior using optical microscopes at home and abroad, there are certain defects, and there is no universally applicable method. Especially for the observation of the epidermis of young leaves and papery leaves, it is difficult to separate the epidermis; for the observation of the epidermis with complex hairs, it is difficult to see the layered hairs and stomata cle...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N1/28
Inventor 刘孟奇高富柳继锋周静王小巧
Owner HENAN UNIV OF CHINESE MEDICINE
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