Method for observing epidermal and internal microstructures of leaf by using transparent leaf

Abstract

The invention relates to a method for observing the epidermal and internal microstructures of a leaf by using a transparent leaf, which has low cost and can accurately observe the microstructures of leaf. The technical scheme in the method comprises the following steps: firstly, processing the leaf, and cleaning and soaking the leaf in ethanol to remove chlorophyll; then soaking in alkaline solution to break the mesophyll cells; decolorizing in sodium hypochlorite; rinsing with flowing water; placing in hydrated chloric acid; taking out the leaf; rinsing with flowing water; dewatering with alcohol solutions with gradient concentrations; placing in xylene solution to enable the leaf to be transparent; and sealing with neutral balsam or water; and observing and picking images with optical microscope to observe the internal and external structure at different levels of the leaf. The method has the advantages of less material selection, low cost, less work load, high success rate and good effect, and is suitable for observation of various leaves.

Description

1. Technical field [0001] The invention relates to a method for observing the microstructure of leaf epidermis and leaf interior by using transparent leaves. 2. Background technology [0002] Morphology and anatomy research is the basic research in botany research, especially the study of leaf epidermis and leaf internal structure has important value for plant classification, plant ecology, plant physiology research and identification of leaf medicinal materials, which cannot be done by other methods. Replacement. However, taking a comprehensive look at the multiple observation methods of leaf epidermis and leaf interior using optical microscopes at home and abroad, there are certain defects, and there is no universally applicable method. Especially for the observation of the epidermis of young leaves and papery leaves, it is difficult to separate the epidermis; for the observation of the epidermis with complex hairs, it is difficult to see the layered hairs and stomata cle...

AI Technical Summary

Problems solved by technology

However, taking a comprehensive look at the various observation methods of leaf epidermis and leaf interior using optical microscopes at home and abroad, there are certain defects, and there is no universally applicable method.

Especially for the observation of the epidermis of young leaves and papery leaves, it is difficult to separate the epidermis; for the observation of the epidermis with complex hairs, it is difficult to see the layered hairs and stomata clearly even with a scanning electron microscope, and for the internal structure of the leaves. Observation, the use of slices to observe the experiment is cumbersome, and some structures such as crystals and different cells cannot be positioned and observed. For the study of leaf veins in the past, the light produced by dyeing in the production of transparent leaves is difficult to penetrate the leaves. As a result, the tiniest leaf veins cannot be seen, resulting in erroneous conclusions, which affect the identification and classification of leaf medicinal plants. Therefore, how to effectively solve the problem of low cost and accurately observe the microstructure of leaf epidermis and leaf interior is a problem for those skilled in the art. A technical problem that has always been hoped to be solved