The invention provides a method for performing 
mating type 
gene identification on a 
basidiospore monocaryon strain which is separated from different hypsizigus marmoreus strains. In the identifying process, in particular application of a flushing reaction, a 
palisade reaction and 
transplanting, the method can primarily identify the 
mating type 
gene of the hypsizigus marmoreus intuitively and quickly without other instruments and equipment. When the method is applied in 
cross breeding, the 
mating type 
gene of 
basidiospore monocaryon parent and progeny strains can be identified conveniently so as to improve the purposiveness and the effectiveness of the 
cross breeding process. The adopted method can shorten the time for identifying the 
mating type gene for 5 to 10 days, reduce the number of comparing-screening strains by 75 percent theoretically in 
cross breeding, improve the 
fecundity, and is more convenient and efficient particularly in application of multiple cross breeding of multiple strains. Then, the method selects the compatible 
basidiospore monocaryon strain having 
clamp connection according to the identification result to carry out biparental crossing, and screens 
hybrid strains according to 
growth speed, growth potential and economical character index, thereby obtaining stable, high yield and high quality 
hybrid strains.