The invention provides a method for performing
mating type
gene identification on a
basidiospore monocaryon strain which is separated from different hypsizigus marmoreus strains. In the identifying process, in particular application of a flushing reaction, a
palisade reaction and
transplanting, the method can primarily identify the
mating type
gene of the hypsizigus marmoreus intuitively and quickly without other instruments and equipment. When the method is applied in
cross breeding, the
mating type
gene of
basidiospore monocaryon parent and progeny strains can be identified conveniently so as to improve the purposiveness and the effectiveness of the
cross breeding process. The adopted method can shorten the time for identifying the
mating type gene for 5 to 10 days, reduce the number of comparing-screening strains by 75 percent theoretically in
cross breeding, improve the
fecundity, and is more convenient and efficient particularly in application of multiple cross breeding of multiple strains. Then, the method selects the compatible
basidiospore monocaryon strain having
clamp connection according to the identification result to carry out biparental crossing, and screens
hybrid strains according to
growth speed, growth potential and economical character index, thereby obtaining stable, high yield and high quality
hybrid strains.