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Method for identifying hypsizigus marmoreus mating type gene

A technology of mating type and true jiji mushroom, which is applied in the field of biological genes to achieve the effect of enhancing purpose and effectiveness and improving fruitability

Inactive Publication Date: 2009-12-23
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the identification method of the mating type gene of P.

Method used

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  • Method for identifying hypsizigus marmoreus mating type gene
  • Method for identifying hypsizigus marmoreus mating type gene
  • Method for identifying hypsizigus marmoreus mating type gene

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Embodiment

[0019] Combine below figure 1 And embodiment further illustrate the present invention.

[0020] 1. PDA medium for parental strain culture, basidiospore isolation and hybrid strain culture: 200g of peeled potatoes, 20g of glucose, 20g of agar, 1000ml of distilled water, natural pH; cultivation medium: 40% sawdust, 24% cottonseed hulls, bran 35%, 1% gypsum, 240g dry weight per bottle, and 60%-62% water content after supplementation;

[0021] Sawdust soaking medium: 200g sawdust soaking juice, add 20g agar, add distilled water to 1000ml.

[0022] 2. Strain material

[0023] True Jiji mushroom SIEF2632 and SIEF3132 were provided by China Committee for Culture Collection of Microbial Cultures, Shanghai Edible Fungi Branch Center of Agricultural Microbiology Center.

[0024] 3. Isolation of basidiospore monokaryotic strains

[0025] Select the fruiting bodies of mature mushrooms SIEF2632 and SIEF3132, cut off the stipe, put the gills flat on the plate, and place them in a 25°C i...

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Abstract

The invention provides a method for performing mating type gene identification on a basidiospore monocaryon strain which is separated from different hypsizigus marmoreus strains. In the identifying process, in particular application of a flushing reaction, a palisade reaction and transplanting, the method can primarily identify the mating type gene of the hypsizigus marmoreus intuitively and quickly without other instruments and equipment. When the method is applied in cross breeding, the mating type gene of basidiospore monocaryon parent and progeny strains can be identified conveniently so as to improve the purposiveness and the effectiveness of the cross breeding process. The adopted method can shorten the time for identifying the mating type gene for 5 to 10 days, reduce the number of comparing-screening strains by 75 percent theoretically in cross breeding, improve the fecundity, and is more convenient and efficient particularly in application of multiple cross breeding of multiple strains. Then, the method selects the compatible basidiospore monocaryon strain having clamp connection according to the identification result to carry out biparental crossing, and screens hybrid strains according to growth speed, growth potential and economical character index, thereby obtaining stable, high yield and high quality hybrid strains.

Description

Technical field: [0001] The invention relates to the technical field of biological genes, in particular, according to the identification method of the mating type gene of the basidiospore monokaryon of chrysanthemum, the initiative and effectiveness of single-single hybrid breeding of chrysanthemum can be improved. Background technique: [0002] True Ji mushroom [Hypsizygus marmoreus (Peck) Bigelow], also known as crab-flavored mushroom, seafood mushroom, Hongxi mushroom, jade mushroom, gelatinous jade mushroom, belongs to Basidiomycotina, Phytomycetes, Agaricaceae, White Mushrooms , Lipophyllum family, and Amygium genus are rare edible fungi that are crisp and tender in texture, delicious in taste, rich in nutrition, and have various health benefits, and are deeply loved by consumers. [0003] At present, some large-scale edible fungus enterprises in my country, Japan, and South Korea have been able to carry out factory cultivation of shimeji mushrooms by controlling temper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04A01H1/02
Inventor 冯志勇刘明广高君辉陈明杰陈辉
Owner SHANGHAI ACAD OF AGRI SCI
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