FoMV-mediated GFP-ATG8 expression vector and application thereof

A GFP-ATG8, expression vector technology, applied in the biological field, can solve problems such as small gene fragments
CN112430619APending Publication Date: 2021-03-02TIANJIN NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN NORMAL UNIVERSITY
Publication Date
2021-03-02

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Abstract

The invention constructs a FoMV-mediated GFP-ATG8 expression vector and application thereof. A method takes TaATG8a as a target for monitoring autophagy, a VOX technology based on FoMV is adopted forexpressing GFP-TaATG8a fusion protein on a wheat plant, the expression situation and subcellular localization situation of the fusion protein in leaves and root tissues are observed through fluorescence, and a technical platform for monitoring the autophagy level on a wheat living plant with overexpression GFPTaATG8 as the target is established. The platform is successfully applied to observationof representation autophagosomes and autophagovesicles in leaf epidermis, mesophyll cells and root cells under hunger and drug treatment, and the invention shows that the vector can be applied to autophagy level monitoring and autophagy function research in the wheat growth and development process or the environmental factor response process. Research results provide technical support for deep exploration of autophagy regulation and control mechanisms and physiological functions of important crop wheat.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a FoMV virus-mediated GFP-TaATG8a expression vector and its application in monitoring the autophagy level of wheat. Background technique

[0002] Autophagy (autophagy for short) is closely related to plant growth, development, aging, cell death and stress response. Monitoring the level of autophagy in cells is a necessary means to carry out research on the regulation mechanism and physiological function of autophagy. In the process of autophagy, the substrate to be degraded in the cytoplasm is wrapped into the autophagosome with a double-membrane structure. With the fusion of the outer membrane of the autophagosome and the membrane of the vacuole (lysosome in animals), the inner membrane and the wrapped autophagosome Substrates (now called autophagic vesicles) enter the vacuolar lumen to be broken down. A variety of autophagy-related (autophagy-related, ATG) factors are involved in t...

Claims

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