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Method for detecting effectiveness of intracellular moisture of plant leaves

A plant leaf and detection method technology, applied in the direction of testing plants/trees, weighing by removing certain components, measuring devices, etc., can solve the problems of inaccurate prediction of plant water deficit information, large environmental factors, etc., to achieve The effect of high precision and mature technology

Active Publication Date: 2020-11-13
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a method for detecting the availability of intracellular water in plant leaves, which evaluates the availability of intracellular water in plant leaves based on changes in leaf thickness and mesophyll cell volume, and analyzes the response of plants to water deficits. response to provide a basis for the detection of water demand time nodes; overcome the shortcomings of existing technologies that cannot accurately predict plant water deficit information and are greatly affected by environmental factors

Method used

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  • Method for detecting effectiveness of intracellular moisture of plant leaves
  • Method for detecting effectiveness of intracellular moisture of plant leaves
  • Method for detecting effectiveness of intracellular moisture of plant leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Select leaves of mulberry trees and mulberry trees with good growth and uniformity, and dry and dehydrate them after being saturated with water. The relative water content of leaves of mulberry tree and mulberry tree at each dehydration time (0, 1, 2, 3, 4, 5 h) was measured by drying method, and the measurement results are shown in Table 1, and the relative water content of leaves was calculated according to formula (1) . Calculate the mesophyll cell thickness MCT according to step 3, and the measurement results are shown in Table 2;

[0039] Table 1 Relative water content (RW, %) of mulberry and mulberry leaves at each dehydration moment

[0040]

[0041] Table 2 Leaf thickness, palisade tissue thickness, spongy tissue thickness and mesophyll cell thickness of mulberry and mulberry leaves at each dehydration time (unit: μm)

[0042]

[0043] Calculate the relative water content, leaf thickness, and mesophyll cell thickness of the leaves at each water loss time...

Embodiment 2

[0054] Take Zhuge Cai and Brassica napus as examples. All steps are the same as implementation case 1.

[0055] Table 5 The relative water content (RW, %) of the leaves of Zhuge Cai and Brassica napus at each dehydration time

[0056]

[0057]

[0058] Table 6 Leaf thickness, palisade tissue thickness, spongy tissue thickness and mesophyll cell thickness of leaves of Zhuge kale and Brassica napus at each dehydration time (unit: μm)

[0059]

[0060] Table 7 The relative changes in leaf thickness, mesophyll cell thickness and leaf relative water content of Zhuge vegetable and Brassica napus leaves at each dehydration time

[0061]

[0062] Note: The value in () represents the increase compared with the previous adjacent water loss moment, and its value is negative.

[0063] Table 8 |V of Zhuge Cai and Brassica napus leaves at each dehydration time MCT | and V RW -V LT the value of

[0064]

[0065]

[0066] It can be seen from Table 8 that the intracellu...

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Abstract

The invention provides a method for detecting the effectiveness of intracellular moisture of plant leaves, and belongs to the technical field of water-saving irrigation and crop information detection.Firstly, subjecting plant leaves to be detected to water saturation and drying treatment; measuring the relative water content, the leaf thickness and the mesophyll cell thickness of the leaf at eachwater loss moment; and calculating the percentage of the relative water content, the leaf thickness and the mesophyll cell thickness of the leaf at each water loss moment in each value of the previous adjacent water loss moment, and further obtaining the relative variation of the relative water content, the leaf thickness and the mesophyll cell thickness of the leaf at each adjacent water loss moment, thereby judging the effectiveness of the intracellular water of the plant leaf. According to the method, the moisture condition of the leaves can be evaluated in time, a basis is provided for advanced prediction of water-saving irrigation crop water-requiring nodes, and the test process is not limited by surrounding environmental factors.

Description

technical field [0001] The invention belongs to the technical field of water-saving irrigation and crop information detection, and in particular relates to a method for detecting the availability of water in cells of plant leaves. Background technique [0002] Affected by global climate change, plants will experience more frequent temporary drought conditions. Water deficit leads to the decline of water availability inside the leaves, which affects the physiological and biochemical processes of plants, resulting in photosynthesis and growth limitation. However, the internal structure and cellular components of plant leaves can respond quickly to achieve water balance and maintain normal plant growth. In agricultural production practice, timely diagnosis of leaf water status is beneficial to the detection of leaf water transportation and utilization process, and the timing of plant water demand can be known in advance to prevent photosynthesis from being inhibited and improv...

Claims

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Application Information

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IPC IPC(8): G01N33/00G01N5/04G01N1/28G01B21/08
CPCG01N33/0098G01N5/045G01N1/30G01B21/08Y02A40/22
Inventor 邢德科李振义吴沿友陈晓乐陈璐赵玉国
Owner JIANGSU UNIV
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