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Complex enzyme preparation for leaching tobacco material and enzyme leaching method

A technology of compound enzyme preparation and leaching method, which is applied in the fields of tobacco, tobacco treatment, tobacco preparation, etc. It can solve the problems of affecting the process operation, difficult to defibrate, and ineffective defibrillation of tobacco leaves.

Active Publication Date: 2013-06-12
HENAN CIGARETTE IND TOBACCO SLICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, two problems are likely to occur in the leaching section of reconstituted tobacco leaves by the papermaking method: 1) It is difficult to defibrate the tobacco leaves, especially the tobacco stems, resulting in uneven stem fibers and affecting the next process operation
2) The dissolution rate is low
Due to the dense structure of mesophyll cells and stalk fibers, it is difficult to achieve the ideal dissolution effect for the contents of tobacco slices and stalks
Although there are research reports on the use of compound amylase, glucoamylase and cellulase to act on the tobacco extraction process, they only have a certain effect on the degradation of biomacromolecules, and have not comprehensively considered the compact structure of tobacco sheets and tobacco stems, improving the Enzyme-catalyzed treatment for the effective dissolution of effective substances, the effect is not very satisfactory

Method used

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  • Complex enzyme preparation for leaching tobacco material and enzyme leaching method

Examples

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Effect test

Embodiment 1

[0011] Compound enzyme preparation for extracting tobacco raw materials, containing 1000U of lysozyme, 0.1U of cellulase, 0.1U of α-amylase, 1000U of proteolytic enzyme and 0.1U of pectinase per 1 mg.

[0012] Extraction method: during extraction, the temperature is controlled at 30°C, and the liquid ratio is 1:5; add 1 mg of compound enzyme preparation per g of tobacco raw material, and stir for 4 hours; after solid-liquid separation, take the solid phase and add water to circulate and extract once according to the weight of the original solid.

Embodiment 2

[0014] Compound enzyme preparation for extracting tobacco raw materials, containing 200 U of lysozyme, 500 U of cellulase, 500 U of α-amylase, 0.1 U of proteolytic enzyme and 1000 U of pectinase per 1 mg.

[0015] The method of use is: control the temperature at 60°C during leaching, and the liquid ratio is 1:10; add 1 mg of compound enzyme preparation per g of tobacco raw material, and stir for 1 hour; after solid-liquid separation, take the solid phase according to the weight of the original solid and add water to circulate and extract twice.

Embodiment 3

[0017] Compound enzyme preparation for extracting tobacco raw materials, containing 0.1U of lysozyme, 1000U of cellulase, 1000U of α-amylase, 400U of proteolytic enzyme and 500U of pectinase per 1 mg.

[0018] The method of use is: during leaching, the temperature is controlled at 40°C, and the liquid ratio is 1:8; 1 mg of compound enzyme preparation is added to each g of tobacco raw material, and stirred for 1 to 3 hours; after solid-liquid separation, the solid phase is extracted according to the weight of the original solid, and then water is circulated for 2 Second-rate.

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Abstract

The invention relates to a complex enzyme preparation for a leaching tobacco material and an enzyme leaching method, and belongs to the technical field of regenerated tobacco processing. The active component of the complex enzyme preparation comprises 0.1-1000U of lysozyme, 1-1000 U of cellulase, 0.1-1000U of alpha-amylase, 0.1-1000 U of proteolytic enzyme and 0.1-1000U of pectinase, wherein the amount is the adding amount in per gram of tobacco material. The temperature in leaching is controlled at 30-60 DEG C, and the liquor ratio is 1:5-10; the enzyme preparation is added to be stirred for 1-4 hours; and after solid-liquid separation, solid phase is taken and water is further added according to original solid weight for circularly extraction for 1-2 times. According to the invention, mesophyll cell and cabo fiber structures, composition, physical properties and the like of tobacco are adequately considered, combined wall breaking effects of lysozyme, pectinase and cellulase are introduced, and enzyme catalytic action of proteolytic enzyme, alpha-amylase and cellulase for degrading biomacromolecule is considered, so that the compact structure of tobacco and straw is dredged, leach of inclusions of materials is increased, the dissolution rate is enhanced and the quality of the extracting liquid is improved.

Description

technical field [0001] The invention belongs to the technical field of reconstituted tobacco leaf processing, relates to a composite enzyme preparation for leaching tobacco raw materials, and also relates to a method for increasing the leaching rate of tobacco raw materials. Background technique [0002] Reconstituted tobacco leaves can not only greatly reduce production costs, but also have many advanced functions such as improving taste and reducing harm in practice. Factors such as raw materials, formulations, material leaching rate and other factors will have an important impact on the physical properties, internal quality and stability of reconstituted tobacco leaves. At the same time, according to the current papermaking production process, the higher the leaching rate of raw materials, the less difficult post-processing, and the lower the processing cost. [0003] At present, two problems are likely to occur in the leaching section of reconstituted tobacco leaves by ...

Claims

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Application Information

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IPC IPC(8): C12N9/88C12N9/50C12N9/42C12N9/36C12N9/26C12N9/18A24B15/20A24B3/14
Inventor 王洪涛王文领尚富德张利涛许红涛尚峰
Owner HENAN CIGARETTE IND TOBACCO SLICE
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