Method for screening SNP markers of Liriodendron genome

A screening method and technology of Liriodendron, applied in the field of genome, can solve the problem of lack of molecular markers, and achieve the effect of reliable quality

Active Publication Date: 2018-11-06
NANJING FORESTRY UNIV
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Problems solved by technology

[0003] Since the 1940s, the origin, distribution, phylogenetic status, asexual reproduction, flowering and fruiting habit, and hybrid breeding of Liriodendron have been studied at home and abroad, and molecular markers such as RAPD and SSR have been developed. There are still

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  • Method for screening SNP markers of Liriodendron genome
  • Method for screening SNP markers of Liriodendron genome
  • Method for screening SNP markers of Liriodendron genome

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Embodiment 1

[0026] A screening method for the SNP marker of Liriodendron tulipifera genome, the specific steps are as follows:

[0027] 1. Materials

[0028] The mating combination of hybrid parents is Liriodendron tulipifera ‘NK’ × Liriodendron chinense ‘LS’, referred to as ‘NK’בLS’, with Liriodendron tulipifera ‘NK’ as the female parent and Liriodendron chinense ‘LS’ as the male parent. The female parent 'NK' is a good provenance in the eastern coast of North America, and the male parent 'LS1' is a provenance in the southeastern inland of China. At the end of April and the beginning of May 2011, the Xiashu Forest Farm of Nanjing Forestry University carried out artificial cross-pollination of Liriodendron tulipifera. 2-3 days after the completion of pollination, when the ovary expands, remove the paper bag. After the aggregated fruit develops and matures in about 140-160 days, harvest it manually. After it is naturally air-dried, remove the seeds of the wing and place it in a cold stor...

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Abstract

The invention discloses a method for screening SNP markers of a Liriodendron genome. F1-generation individuals obtained from Liriodendron tulipifera 'NK' and Liriodendron 'LS' by hybridization are taken as a material, a Liriodendron reference genome obtained by sequencing is taken as the basis, PCR amplification primers are designed by Primer 5.0 software, amplification efficiency and specificityof the primers are verified, 27 candidate SNP loci are selected for primer design, a screening result is detected by agarose gel electrophoresis after PCR amplification, provenance material are verified, and finally, the SNP markers of the Liriodendron genome are obtained. 27 SNP loci are selected from a Liriodendron genome database for primer synthesis, products are subjected to PCR amplificationdetection and sent out for sequencing, a result shows that all the loci are real SNP loci, which means that the SNP loci are reliable in quality, used standards are better, and the obtained high-quality SNP markers can be applied to follow-up work such as genetic linkage maps and the like.

Description

technical field [0001] The invention belongs to the technical field of genomes, and in particular relates to a screening method for SNP markers of Liriodendron tulipifera genome. Background technique [0002] Liriodendron belongs to the genus Liriodendron in the family Magnoliaceae. Today there are only two species, namely Chinese tulipwood (L.chinese Sarg.) (also known as mandarin tree) and North American tulipifera (L.tulipifera L.). Liriodendron has grown in northern Europe, Greenland, and Alaska at higher latitudes in the northern hemisphere in the late Jurassic of the Mesozoic; it was widely distributed in Eurasia and North America in the Tertiary of the Cenozoic; after the glaciers of the Quaternary It is only distributed in the south of my country and the southeast of North America, becoming a typical relict plant. Liriodendron tulipifera and Liriodendron tulipifera have a certain geographical isolation, but their interspecific compatibility is high (Wang Zhangrong, ...

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6869
Inventor 施季森陆叶陈金慧龙晓飞杨立明肖保荣刘阳陈新颖张稼霁
Owner NANJING FORESTRY UNIV
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