Method for screening SNP markers of Liriodendron genome

Abstract

The invention discloses a method for screening SNP markers of a Liriodendron genome. F1-generation individuals obtained from Liriodendron tulipifera 'NK' and Liriodendron 'LS' by hybridization are taken as a material, a Liriodendron reference genome obtained by sequencing is taken as the basis, PCR amplification primers are designed by Primer 5.0 software, amplification efficiency and specificityof the primers are verified, 27 candidate SNP loci are selected for primer design, a screening result is detected by agarose gel electrophoresis after PCR amplification, provenance material are verified, and finally, the SNP markers of the Liriodendron genome are obtained. 27 SNP loci are selected from a Liriodendron genome database for primer synthesis, products are subjected to PCR amplificationdetection and sent out for sequencing, a result shows that all the loci are real SNP loci, which means that the SNP loci are reliable in quality, used standards are better, and the obtained high-quality SNP markers can be applied to follow-up work such as genetic linkage maps and the like.

Description

technical field [0001] The invention belongs to the technical field of genomes, and in particular relates to a screening method for SNP markers of Liriodendron tulipifera genome. Background technique [0002] Liriodendron belongs to the genus Liriodendron in the family Magnoliaceae. Today there are only two species, namely Chinese tulipwood (L.chinese Sarg.) (also known as mandarin tree) and North American tulipifera (L.tulipifera L.). Liriodendron has grown in northern Europe, Greenland, and Alaska at higher latitudes in the northern hemisphere in the late Jurassic of the Mesozoic; it was widely distributed in Eurasia and North America in the Tertiary of the Cenozoic; after the glaciers of the Quaternary It is only distributed in the south of my country and the southeast of North America, becoming a typical relict plant. Liriodendron tulipifera and Liriodendron tulipifera have a certain geographical isolation, but their interspecific compatibility is high (Wang Zhangrong, ...

AI Technical Summary

Problems solved by technology

[0003] Since the 1940s, the origin, distribution, phylogenetic status, asexual reproduction, flowering and fruiting habit, and hybrid breeding of Liriodendron have been studied at home and abroad, and molecular markers such as RAPD and SSR have been developed. There are still problems such as the lack of molecular markers suitable for the genetics research of Liriodendron, so the development of SNP markers with high polymorphism will help the investigation and evaluation of genetic resources of Liriodendron plants and the research of hybrid breeding

Images