Method for extracting horseradish tree protein from horseradish tree seed

A technology of Moringa oleifera seed and protein, which is applied in the biological field, can solve the problems of no reports, etc., and achieve the effect of easy handling, low COD value and good quality

Inactive Publication Date: 2009-08-19
昆明金大毫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aqueous enzymatic extraction of moringa oil and moringa protein in moringa seeds, no report at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A, take by weighing the Moringa kernel powder 50g that pulverizes and screen with 60 mesh standard test sieves, press Moringa kernel powder: the mass ratio of distilled water=1: 12, add distilled water 600g therein, stir 6min with the rotating speed of 8000r / min , with a concentration of 0.1mol / L hydrochloric acid solution to adjust the pH value of the mixture to 4;

[0023] B. In the mixture of the above step A, add liquid cellulase 0.5g according to the mass ratio of Moringa kernel powder: enzyme = 1: 0.01, 40°C constant temperature enzymolysis reaction in water bath for 12h, while constantly stirring, stirring speed 60r / min;

[0024] C. Take out the enzymatic hydrolyzed mixture in step B and put it into a centrifuge, and perform centrifugation at a speed of 4000r / min for 8 minutes. The upper layer is an oil layer, and the Moringa oil is obtained after separation. The lower layer is water and solid cake, and the water and cake are taken out. , filter with rapid qual...

Embodiment 2

[0027] A, take by weighing the Moringa oleifera kernel powder 500g that pulverizes and screen with 60 mesh standard inspection sieves, according to the mass ratio of Moringa oleifera kernel powder: distilled water=1: 13, add distilled water 6500g wherein, 10000r / min high-speed stirring 8min, use Concentration is the NaOH solution of 0.1mol / L to adjust the pH value of the mixture to be 8;

[0028] B. In the mixture of the above step A, add 25 g of compound cellulase according to the mass ratio of Moringa kernel powder: enzyme = 1: 0.05, 50 ° C constant temperature enzymolysis reaction in a water bath for 48 hours, while constantly stirring, stirring speed 100r / min .

[0029] C. Take out the enzymatically hydrolyzed mixture of step B and put it into a centrifuge, and centrifuge for 9 minutes at a speed of 6000r / min. The upper layer is an oil layer, and the Moringa oil is obtained by separation. The lower layer is water and solid cake, and the water and cake are taken out. Use a...

Embodiment 3

[0032] A, take by weighing the Moringa kernel powder 5kg that pulverizes and sieve with 60 mesh standard test sieves, according to the mass ratio of Moringa kernel powder: pure water=1: 15, add pure water 75kg therein, 12000r / min high-speed stirring 10min , with a concentration of 0.1mol / L hydrochloric acid to adjust the pH value of the mixture to 6;

[0033] B, in the mixture of above-mentioned steps A, add acid xylanase 500g by the mass ratio of Moringa kernel powder: enzyme=1:0.1, 70 ℃ of constant temperature enzymolysis reaction 24h in the water bath, constantly stir simultaneously, stirring speed 150r / min.

[0034] C. Take out the enzymatically hydrolyzed mixture in step B and put it into a centrifuge, and centrifuge it at a speed of 8000r / min for 10 minutes. The upper layer is an oil layer, and the Moringa oil is obtained by separation. The lower layer is water and solid cake, and the water and cake are taken out. Use a 150-mesh nylon sieve to filter under normal press...

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PUM

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Abstract

The invention provides a method for extracting horseradish protein from horseradish seeds. The method is characterized by comprising the following steps: horseradish kernel powder is added with water for mixing according to 1:12-15 mass ratio of the horseradish kernel powder to water and pH value is adjusted to 4-8; the obtained mixture is added with enzyme based on 1:0.01-0.1 mass ratio of the horseradish kernel powder to the enzyme, enzymolytic reaction is performed in water bath at constant temperatures of 40-70 DEG C for 12-48h to separate horseradish grease, water phase and solid cake; and the pH value of the water phase is adjusted to 7-9 to centrifugally separate supernatant, then the pH value of the supernatant is adjusted to 10-12 for centrifugal separation, the supernatant is discarded, and the precipitates which are obtained twice are combined, dried and cooled to obtain the horseradish protein. The method helps simultaneously obtain horseradish oil and high-quality horseradish protein under the mild condition without organic solvent, and is safe, energy-saving and environmental-friendly.

Description

technical field [0001] The invention relates to a protein extraction method, in particular to a method for extracting Moringa oleifera protein from Moringa oleifera seed kernels with an enzymatic method, and belongs to the field of biotechnology. Background technique [0002] In addition to being rich in high-grade edible oils, Moringa seeds also contain about 38% Moringa protein in the seeds. The nutritional value of moringa protein is higher than that of milk and eggs, and it basically does not contain cholesterol. In addition, moringa protein can also be used as a water purifier, and its value is quite high. Therefore, extracting moringa protein from moringa seeds is a very important topic . At present, the main ways to extract vegetable oil from oilseeds are pressing and leaching. Before pressing or leaching, the oil should be crushed, crushed, flaked, baked or steamed, and then the cell structure of the oil should be destroyed by mechanical and thermal means to achiev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415
Inventor 段琼芬马李一张重权王有琼
Owner 昆明金大毫生物科技有限公司
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