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Plant hormone quantitative detection kit as well as detection method and application thereof

A plant hormone, quantitative detection technology, applied in the research field, can solve the problems of resource waste, low instrument sensitivity, low sensitivity, and only one plant hormone can be detected at a time

Active Publication Date: 2021-04-16
上海鹿明生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing detection methods include ELISA, GCMS, LCMS and other methods. ELISA is a qualitative and quantitative detection method that uses the specificity of antigen-antibody binding to carry out immune reactions. For hormone detection, it has poor specificity, complicated operation, and high throughput. low, and can only detect one plant hormone at a time; while GCMS has the disadvantages of derivatization and low sensitivity of the instrument; and the most commonly used LCMS also requires complex pre-treatment enrichment, or after Sensitization technology of mass spectrometry to improve the detection sensitivity of plant hormones
In addition, due to the extreme instability of plant hormones, in order to avoid the degradation of the target substance during the pretreatment process, a series of protective measures such as avoiding light, low temperature, and adding antioxidants are required. a waste

Method used

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  • Plant hormone quantitative detection kit as well as detection method and application thereof
  • Plant hormone quantitative detection kit as well as detection method and application thereof
  • Plant hormone quantitative detection kit as well as detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Step 1: Sample pretreatment

[0102] Required reagents: extractant 1, extractant 2, reconstitution solvent

[0103] Extraction steps:

[0104] 1. First take about 50 mg of the freeze-dried Arabidopsis leaf sample, and add 500 μL of extractant 1;

[0105] 2. Then add 2 steel balls and grind with a grinder for 2 minutes (pre-cooling in advance);

[0106] 3. Then place the ground sample at -20°C for 20 minutes, and ultrasonically in an ice bath for 30 minutes;

[0107] 4. Add 1mL extractant 2, place at -20°C for 20min, sonicate in an ice bath for 5min, and vortex for 1min;

[0108] 5. Then centrifuge for 5 minutes (4°C, speed 13000rpm), remove 900 μL of the lower layer, (take in two times, 450 μL each time, put in a 1.5 mL brown LCMS sample bottle) and evaporate to dryness;

[0109] 6. Finally, add 200 μL of reconstitution solvent to redissolve (sonicate in ice bath for 1 min), centrifuge for 5 min (4°C, speed 13000 rpm), take 150 μL of the supernatant into a brown LC ...

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Abstract

The invention belongs to the research fields of plant growth and development, molecular heredity, cytobiology and the like, and relates to a plant hormone quantitative detection kit, a detection method and application. The plant hormone quantitative detection kit is used for accurately and quantitatively detecting 10 plant hormones in a plant sample. The plant hormone quantitative detection kit disclosed by the invention is small in required sample volume, simple in sample pretreatment, strong in specificity and matrix interference resistance, short in detection time, high in flux, high in detection precision and low in cost. The invention also discloses a method for quantitatively detecting plant hormones by using the kit.

Description

technical field [0001] The invention belongs to the research fields of plant growth and development, molecular genetics and cell biology, and relates to a plant hormone quantitative detection kit, a detection method and an application thereof. Background technique [0002] Phytohormones are a very important class of substances in plants, they play a key role in regulating plant development and responses to various biotic and abiotic stresses. However, their content in plants is extremely low, and they are unstable and easily degraded. Therefore, the quantitative detection of phytohormones has always been a challenge. At present, the existing detection methods include ELISA, GCMS, LCMS and other methods. ELISA is a qualitative and quantitative detection method that uses the specificity of antigen-antibody binding to carry out immune reactions. For hormone detection, it has poor specificity, complicated operation, and high throughput. low, and can only detect one plant hormo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
Inventor 丁山季佩佩尹娜娜付艳蕾舒烈波
Owner 上海鹿明生物科技有限公司
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