56results about How to "High detection precision" patented technology

The invention relates to a quantitative detection device based on fibrous-membrane gathering and separation and a detection method thereof. The quantitative detection device comprises a separation testing cup, a reaction cup, an analysis buffer solution and a cleaning solution; wherein the separation testing cup comprises a receiving tank, a water absorption cup and millipore filters between them. A capture agent and a labeling agent are solidified on the bottom of the reaction cup and the capture agent is fixed on the surface of latex beads. The detection method comprises: conjugation reaction, separation, cleaning and detection. In the invention, not only the characteristics of convenient operation and rapid detection of immune infiltration (IF) and immunochromatography (IC) are maintained, but also precision and sensitivity of the detection are obviously better than that of IF and IC. Therefore, quantitative immunodetection and nucleic acid hybridization analysis can be implemented effectively. The invention can be used for the quantitative detection of substances such as protein, nucleic acid, small molecule and microbe.

The invention provides a homogeneous phase immunoassay POCT detection technique and a system using same. The homogeneous phase immunoassay POCT detection technique has the characteristics of high sensitivity, high precision and wide range of chemiluminescence immune assay technique and rapidness, portability and the like of a POCT detection technique; in addition, as pure liquid phase detection, the homogeneous phase immunoassay POCT detection technique overcomes the problem that CV is relatively high due to an NC membrane, so that the detection precision is high, generally the CV can be controlled to be within 5%, and the level of precision of the POCT detection technique can reach or even exceed that of the chemiluminescence immune assay technique. In the system using the homogeneous phase immunoassay POCT detection technique, a reagent card and a POCT analyzer are separately designed, a sample to be tested is acquired by using the reagent card, and the system is convenient to carry over.

The present invention relates to a method for detecting acrylamide in food, and particularly to a quick fluorescent detecting method for detecting AM content in toasted, fried, or baked foods, which is characterized in easy operation, high sensitivity and repeatability, quick and accurate detection, high application value, high economical efficiency and high reliability, and easy application. The method comprises: loading pulverized sample into a container, adding normal hexane, performing ultrasonic degreasing, and blowing dry the residual normal hexane to obtain a product A; adding NaCl solution into the product A, performing ultrasonic extraction and centrifugation, and then re-extracting from the residue with NaCl solution, and combining the liquid extract; adding NaOH solution and then sodium hypochlorite solution into the liquid extract, conditioning the mixed solution after reaction with a phosphate buffer solution to alkaline, and finally adding fluorescamine solution and mixing, and loading the mixed solution to a luminoscope to test.

The invention relates to the technical field of biochemical reagent combined calibration. The quality and speed of clinical examination are increased, the labor intensity of inspectors is lowered, samples and reagents are reduced, the detection precision is improved, errors are reduced, and the standardization of the clinical examination is realized. A series of experiments prove that biofilms with different molecular weights have different permeation degrees for different substances and within different times. The content of each substance in serum is increased by biofilm serum concentration,the substances with different molecular weights can permeate the biofilms with the different molecular weights, and target substances are calibrated in a high-concentration combined way; the different substances are mixed together, the concentration required in each item of the combined calibration is achieved, the dissolution and valuing problems of special substances such as total bilirubin, bile acid, triglyceride and uric acid are well solved, the problems of unstable calibration and clinical trench effect are also solved, and the clinical measured values are enabled to be close to true values.

The invention aims to provide a multiple PCR detection kit and a method for identifying susceptible bacteria species for intracranial post-operation to eliminate endogenous nucleic acid contamination. The detected bacteria species are the following common bacteria: staphylococcus aureus, enterococcus faecalis, streptococcus pneumoniae, escherichia coli, bacillus pyocyaneus, klebsiella pneumoniae and acinetobacter baumannii. Meanwhile, the invention provides the method to eliminate the endogenous nucleic acid contamination in a raw material of a PCR system and exclude a false positive experimental result of a PCR caused by the endogenous nucleic acid contamination. The detecting method using the kit has the advantages of that the operation is simple and fast, the detection cost is lowered, the detection result is accurate, and the false positive result is reduced. A negative control and a positive control are added in the amplification step, so that the judgment of false positive and false negative is effectively improved, and the detection accuracy is higher.

The invention provides a method for detecting contents of vanadium, tungsten and titanium elements in an SCR (selective catalytic reduction) catalyst. The method comprises the following step of usinghydrochloric acid, nitric acid and hydrofluoric acid to digest a to-be-detected sample of the SCR catalyst. The method for detecting the contents of the vanadium, tungsten and titanium elements in theSCR catalyst has the advantages that a detection plan is determined by sample preparation, specimen dissolving method, standard solution preparation and the like; the to-be-detected sample of the SCRcatalyst is digested by the hydrochloric acid, nitric acid and hydrofluoric acid, so that the vanadium, tungsten and titanium elements in the SCR catalyst can be fully dissolved, and the contents ofthe three elements can be detected; the detection accuracy is further improved; simplicity, rapidity, accuracy and reliability are realized, and the like; after the contents of vanadium, tungsten andtitanium elements are detected, an efficient and feasible plan can be conveniently made for the recycling of vanadium, tungsten and titanium elements, and the recycling rate of vanadium, tungsten andtitanium elements can be convenient to calculate accurately .

The invention provides a method for detecting content of vanadium element in an SCR (selective catalytic reduction) catalyst. The method comprises the following step of using hydrochloric acid and hydrogen peroxide to digest a to-be-detected sample of the SCR catalyst. The method for detecting the content of the vanadium element in the SCR catalyst has the advantages that a detection plan is determined by sample preparation, specimen dissolving method, standard solution preparation and the like; the to-be-detected sample of the SCR catalyst is digested by the hydrochloric acid and hydrogen peroxide, so that the vanadium element in the SCR catalyst can be fully dissolved; the detection accuracy is further improved; the simplicity, rapidity, accuracy and reliability are realized, and the like.

The invention relates to a double-labeled immunodetection method containing an internal reference, which is used for detecting a target protein in a sample. The method comprises the following steps of labeling a second binding protein of a target protein and an internal reference quality controller by adopting different markers, and substituting a marker signal value into a correction formula established by an inventor for calculation; substituting the calculation result into a fitting curve established based on the standard substance, and reversely solving the target protein concentration of the actual sample. The method can effectively reduce a problem of poor precision caused by the influence of interference factors on the detection result of the immunodetection reagent so that detection precision can be improved to CV less than 5%; based on the method, the inventor also designs a double-labeling kit for detecting troponin I, the influence of interference factors on a detection result can be effectively reduced, so detection precision is improved, and a powerful guarantee is provided for improving detection accuracy of existing immune quantitative analysis.

The invention relates to a multi-point-location on-line automatic collection system for gas in a livestock and poultry field area. The automatic collection system comprises a plurality of groups of gas production heads, a plurality of groups of gas inlet pipelines, a gas collection box and a gas backwashing system. Gas samples at all monitoring point locations of a farm enter the gas inlet pipelines through the gas production heads arranged at the top end of a telescopic supporting frame and then are conveyed into the gas collection box through the gas inlet pipelines, and the collected gas samples are switched in a gas sampling box through a gas inlet switching valve and then sequentially enter a gas monitoring instrument. According to a multi-point-location on-line automatic collection method for gas in a livestock and poultry field area, automatic online continuous collection of gas samples at a plurality of monitoring point locations in a livestock and poultry farm can be realizedautomatically and real-time continuous measurement of a gas on-line monitoring instrument can be realized. Compared with a conventional method, the method has the advantages that the gas collection process of the livestock and poultry farm is simplified, labor is saved, and the gas detection precision is improved.

The invention discloses a kit for quantitative detection of H-FABP and a H-FABP quantitative detection method. The kit for the quantitative detection of the H-FABP comprises a reagent strip, wherein aplurality of accommodating groove bodies are formed in the reagent strip; and the kit further comprises a magnetic particle reagent, a labeling reagent, a cleaning solution and a luminous substrate of alkaline phosphatase which are respectively pre-stored in different accommodating tank bodies, the magnetic particle reagent is prepared from magnetic beads coated with an anti-H-FABP primary antibody, and the labeling reagent is prepared from an anti-H-FABP secondary antibody subjected to activation treatment and alkaline phosphatase subjected to activation treatment. When the kit is used for detection, the operation is simple, the result is fast, the detection result is accurate, and the detection precision is high.

The invention provides electrochemical detection test paper of triglyceride and a preparation method and application thereof, and a detection sensor with the same. The electrochemical detection test paper comprises an ink layer and a reaction enzyme layer; and the composition raw materials of the ink layer comprise carbon ink, an electronic mediator and a carbon nanotube. According to the providedelectrochemical detection test paper, in actual testing, the current gradient change is great, the precision is high, storing at room temperature can be achieved, and the stability is good.

The invention relates to a method for detecting parecoxib sodium genotoxic impurities. The detection method comprises the following steps of dissolving an R1-M reference substance, an R1-C reference substance and an R1-N reference substance separately to obtain three kinds of impurity reference substance solutions; dissolving parecoxib sodium to be tested to obtain a sample solution to be tested;performing liquid chromatography / mass spectrometry determination on the three kinds of impurity reference substance solutions and the sample solution to be tested; the chromatographic conditions include: the mobile phase A is volatile acid or buffer solution, and the mobile phase B is acetonitrile; and the mass spectrometry conditions include: selecting an electrospray ionization, a positive ion scanning mode, the declustering potential is 25 to 200V, the collision energy is 10 to 100eV, the capillary voltage is 2000 to 6000V, the capillary temperature is 250 to 400 DEG C, and the drying gas temperature is 250 to 600 DEG C. The detection limit of the above method reaches 0.3ppm, the limit of quantification reaches 1ppm, and the quality control of pareximab sodium bulk drugs can be well carried out.

The invention discloses an analysis method of phosphorus content in phosphoric pig iron, and relates to the technical field of component analysis of pig iron. According to the method, a method utilizing spark source atomic emission spectrometer is used for detecting, so that the relative standard deviation is small, and the accuracy is high; compared with an inductively coupled plasma atomic emission spectrometry with high accuracy, the method has the advantages that the accuracy in detecting phosphorus content in phosphoric pig iron is high; and the requirement of detection on outsourced pigiron is met.

The invention discloses an exenatide detection kit. The exenatide detection kit comprises exenatide monoclonal antibodies, an ELISA (enzyme-linked immunosorbent assay) plate, a positive reference substance, a negative reference substance, a scrubbing solution, a stop solution and a developing solution, wherein the exenatide monoclonal antibodies comprise an exenatide monoclonal antibody coating the ELISA plate and an exenatide monoclonal antibody marked with horse radish peroxidase. When the detection kit is used for detecting concentration of exenatide of a to-be-detected sample, the sensitivity of the detection kit can be up to 1 ng / mL, the sensitivity is high, a detection result is accurate, besides, a detection object can be serum of human or animals, and an exenatide preparation is wide in application range.

The invention relates to the technical field of chemiluminiscence detection, and particularly discloses a receptor reagent for homogeneous chemiluminiscence detection and application of the receptor reagent. The receptor reagent comprises receptor particles capable of reacting with active oxygen to generate a detectable chemiluminiscence signal, wherein the particle size distribution variable coefficient C.V value of the receptor particles in the receptor reagent is greater than or equal to 5%. Compared with the prior art, the performance of a kit of the receptor reagent is greatly improved, and the kit not only has ultrahigh sensitivity, but also has a very wide detection range.

The invention relates to a homogeneous chemiluminescence POCT detection method and a device using same detection method. The method comprises the following steps: S1, mixing a to-be-detected sample with a receptor reagent and a donor reagent to form a to-be-detected mixture; S2, exciting the to-be-detected mixture to perform chemiluminescence by utilizing energy or an active compound, and immediately measuring the signal intensity of chemiluminescence, wherein the donor reagent comprises donor microspheres, and the donor microspheres can generate active oxygen in an excited state; the receptorreagent comprises receptor microspheres, and the receptor microspheres can react with the active oxygen to generate a detectable chemiluminescence signal; and the particle size of the donor microspheres is equal to that of the receptor microspheres. The method has the characteristics of high sensitivity, high precision and wide range of a chemiluminescence analysis technology, and meanwhile, thePOCT detection technology is rapid and portable.

The invention discloses a vacuum tank detection anti-explosion device, and belongs to a plastic tank pressure detecting device. The vacuum tank detection anti-explosion device comprises a box frame, avacuum tank fixing device and a pneumatic pump. The vacuum tank fixing device is fixedly installed on the box frame. The pneumatic pump is arranged in an inner cavity of the box frame. During working, a vacuum tank is fixed by the vacuum tank fixing device, a joint of the pneumatic pump is connected with an air inlet hole of the vacuum tank, so that a pressure test of the vacuum tank is realizedby the pneumatic pump. According to the vacuum tank detection anti-explosion device, the technical problem that an existing safety detection and protection device is high in the cost, complex in the structure and not suitable for production needs of small and medium sized car enterprises and part manufacturers are solved. Compared with an existing air pressure anti-explosion detection device, thevacuum tank detection anti-explosion device has the characteristics of simple structure, convenient operation, high detection safety performance, high detection precision, and simultaneous detection of two kinds of testing items including positive pressure and negative pressure, and at the same time, an inspector can observe test results at a close range, and popularization and use for the small and medium sized part manufacturers are facilitated.

The invention belongs to the research fields of plant growth and development, molecular heredity, cytobiology and the like, and relates to a plant hormone quantitative detection kit, a detection method and application. The plant hormone quantitative detection kit is used for accurately and quantitatively detecting 10 plant hormones in a plant sample. The plant hormone quantitative detection kit disclosed by the invention is small in required sample volume, simple in sample pretreatment, strong in specificity and matrix interference resistance, short in detection time, high in flux, high in detection precision and low in cost. The invention also discloses a method for quantitatively detecting plant hormones by using the kit.

The invention discloses a homogeneous chemiluminiscence detection method. The method comprises the following steps: detecting a detected object by adopting a reaction principle of a sandwich method; the method is characterized in that the kit for detecting a detected object is composed of nano-gold photosensitive particles and luminescent particles, and the nano-gold photosensitive particles are conjugates obtained by coupling a compound of the nano-gold particles and a photosensitizer with a compound capable of generating specific binding pairing; the light-emitting particles are conjugates of light-emitting microspheres and bioactive molecules such as antibodies. According to the invention, gold nanoparticles are used as a carrier; a water-soluble photosensitizer is electrostatically adsorbed, and energy is transferred to photosensitizer molecules combined with the water-soluble photosensitizer in a non-radiative energy transfer mode, so the water-soluble photosensitizer is obtained.The molecules reach an excited state, so triplet-state oxygen is excited to a singlet state, the singlet-state oxygen content is increased, the difference between the wavelength of excitation light and the emission wavelength is increased, the detection identification degree is improved, and the detection precision, accuracy and sensitivity are enhanced.

The invention provides an automatic mobile phone middle plate detection device. The automatic mobile phone middle plate detection device is arranged on a conveyor belt of a mobile phone middle plate,wherein supporting frames are arranged on the two sides of the conveyor belt, an industrial computer, a vidicon and an illumination lamp are arranged on the supporting frames, and the industrial computer has a main control CPU and a display screen; the conveyor belt transmits a mobile phone middle plate to be detected, the illumination lamp illuminates the mobile phone middle plate, the camera ofthe vidicon is aligned with the mobile phone middle plate for photographing, the vidicon is in signal connection with the main control CPU to transmitting a photographed image to the main control CPU,and the main control CPU receives the image and compares the image with a standard image stored on the main control CPU so as to pick out unqualified products.

The invention discloses a receptor reagent and application thereof. The receptor reagent comprises a first buffer solution and receptor particles which suspend in the first buffer solution and can react with active oxygen to generate chemiluminescence signals, wherein the surfaces of the receptor particles are combined with biomolecules, and a particle size distribution variable coefficient C.V value of the receptor particles in the receptor reagent is not lower than 5% and not higher than 20%; and the sugar content in each milligram of the receptor particles is not higher than 40 [mu] g. The receptor reagent has the beneficial effects that the particle size distribution variable coefficient C.V value of the receptor particles in the receptor reagent is not lower than 5% and not higher than 20%; furthermore, the receptor reagent not only can meet the commercial requirement of mass production, but also has ultrahigh sensitivity and very wide detection range. Meanwhile, the sugar content in the receptor particles is controlled, so that the influence of sugar on a detection signal is reduced, the detection precision is improved, and the production cost is further reduced.

The invention provides a method for detecting the total arsenic content in zeolite powder by using a microwave digestion method to pre-treat a zeolite powder sample and using inductively coupled plasma mass spectrometry as an analysis method. The method comprises the following steps: (1) pretreatment of a sample: adding a zeolite powder sample into a digestion solution, and carrying out microwavedigestion to obtain a test solution, wherein the digestion solution is a mixed solution of nitric acid and hydrogen peroxide; and (2) detection of the total arsenic content: determining the total arsenic content by inductively coupled plasma mass spectrometry. Compared with a traditional detection method taking a hydrochloric acid digestion method as a pretreatment method, the method of the invention has the advantages that when the microwave digestion method is taken as the pretreatment method, a plurality of samples can be treated at the same time, so that the detection efficiency is improved, the digestion process is controlled by a program, the personal error is small, the digestion degree is high, and the operation is simple, convenient, stable and safe; and the inductively coupled plasma mass spectrometry is used for detecting and analyzing the arsenic content, so that the sensitivity and detection result accuracy are high, the analysis speed is high, and the stability is good.

The invention relates to an altimeter placing platform which comprises supporting legs, a first placing platform and a second placing platform lower than the first placing platform. One edge of the second placing platform abuts against the first placing platform, an upper placing platform face and a lower placing platform face are arranged on the first placing platform, the side edge of the upper placing platform face and the side edge of the lower placing platform face are respectively provided with a first square hole, and a second square hole is formed in the side edge, abutting against the first placing platform, of the second placing platform. The altimeter placing platform is simple in structure, the outline, the height and the size of the altimeter placing platform are strictly designed according to actual measuring needs, the placing platform is beneficial for reducing measuring errors, and detecting precision is improved. The altimeter placing platform is ingeniously designed to be the two placing platforms with different heights, two altimeters can be placed on the altimeter placing platform and are used for detecting tooling parts simultaneously, and detecting precision is improved.

The invention relates to the technical field of immunological detection, in particular to a method for detecting DNASE1L3 based on a magnetic particle chemiluminescence immunoassay. The method comprises the following steps: preparing a biotinylated anti-DNASE1L3 antibody; preparing an enzyme-labeled anti-DNASE1L3 antibody; combining the biotinylated anti-DNASE1L3 antibody with the anti-DNASE1L3 antibody in a sample to be detected; adding streptavidin-coated magnetic particles, reacting, washing under the action of magnetic force, and removing uncombined substances; adding an enzyme-labeled anti-DNASE1L3 antibody, reacting, washing under the action of magnetic force, and removing uncombined substances; adding a buffer solution and a luminous substrate solution for reaction, detecting the luminous intensity on a luminoscope, and calculating the concentration of the DNASE1L3 in the sample. The preparation method and application of the mortise and tenon joint structure g-c3n4moo3 compositephotocatalytic material can rapidly realize quantitative analysis of DNASE1L3, and have the advantages of high detection sensitivity and strong specificity.

The invention discloses a horizontal automatic sampling device for ultraviolet fluorescence sulfur, which comprises a sampling mechanism and a sample injection mechanism. The sampling mechanism comprises a base, a rotating disc, a plurality of sample bottles, a sampling needle and a sampling pipeline, a plurality of fixed clamping grooves are formed in the rotating disc, a plurality of sample bottles are respectively arranged in the fixed clamping grooves in a matched manner, the sampling needle is communicated with the sampling pipeline, and the sampling needle is driven by a stepping motor to vertically move up and down. The sample injection mechanism comprises a sample loop, a six-way valve, an emptying port, a negative pressure pump, a carrier gas inlet and a connecting mechanism, sixvalves of the six-way valve are respectively communicated with the sampling pipeline, one end of the sample loop, the carrier gas inlet, the connecting mechanism, the other end of the sample loop andthe emptying port, and the sample injection mechanism is communicated with a sulfur and nitrogen analyzer through the connecting mechanism. The device provided by the invention can realize automatic and horizontal sample injection on the basis of the existing manual equipment, and can improve the detection precision while improving the detection work efficiency.

The invention provides an SDF-1 determination kit. The SDF-1 determination kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises 10-100 mmol / L of a buffer solution, 0.05-1 g / L of a preservative, 30-50 g / L of a coagulant, 5-15 g / L of a stabilizer and 2-5 mL / L of a surfactant; the reagent R2 comprises 40-100 mmol / L of a buffer solution, 1-2 g / L of a preservative, 0.5-1 g / L of a stabilizer, 2-5 mL / L of a surfactant and 30-50 mg / L of anti-human SDF-1 antibody mixed latex particles; wherein latex particles in the anti-human SDF-1 antibody mixed latex particles adopt two different particle sizes. The invention also provides a preparation method and a determination method of the SDF-1 determination kit. The kit is used for measuring the content of SDF-1, is simple and convenient to operate, short in detection time and low in cost, and also has relatively high sensitivity, precision and stability.

The invention discloses a method for detecting odor substances in a water body, belongs to the field of water body detection, and particularly relates to surface mesoporous silicified carbon fibers obtained by pretreating and acidizing carbon fibers, soaking the carbon fibers in a functional treatment solution, calcining the carbon fibers, soaking the calcined carbon fibers in a silicate ester solution and finally calcining the calcined carbon fibers. The method comprises the following steps: preparing a carbon fiber extraction column from surface mesoporous silicified carbon fibers, adsorbing odor substances in a water body, then carrying out desorption treatment, and detecting by adopting LC-MS / MS; the functional treatment liquid contains silanized ionic liquid, ammonia water and 2-ketone-D-glutamic acid hemicalcium salt; the silanized ionic liquid is prepared from 1-allyl imidazole and 3-chloropropyl trimethoxy silane, and the silanized ionic liquid is prepared from 1-allyl imidazole and 3-chloropropyl trimethoxy silane. According to the method, the detection limit of the odor substances in the water body is low, and the detection accuracy and precision of the odor substances in the water body are relatively high.