56results about How to "High detection precision" patented technology

The invention relates to a quantitative detection device based on fibrous-membrane gathering and separation and a detection method thereof. The quantitative detection device comprises a separation testing cup, a reaction cup, an analysis buffer solution and a cleaning solution; wherein the separation testing cup comprises a receiving tank, a water absorption cup and millipore filters between them. A capture agent and a labeling agent are solidified on the bottom of the reaction cup and the capture agent is fixed on the surface of latex beads. The detection method comprises: conjugation reaction, separation, cleaning and detection. In the invention, not only the characteristics of convenient operation and rapid detection of immune infiltration (IF) and immunochromatography (IC) are maintained, but also precision and sensitivity of the detection are obviously better than that of IF and IC. Therefore, quantitative immunodetection and nucleic acid hybridization analysis can be implemented effectively. The invention can be used for the quantitative detection of substances such as protein, nucleic acid, small molecule and microbe.

The invention provides a homogeneous phase immunoassay POCT detection technique and a system using same. The homogeneous phase immunoassay POCT detection technique has the characteristics of high sensitivity, high precision and wide range of chemiluminescence immune assay technique and rapidness, portability and the like of a POCT detection technique; in addition, as pure liquid phase detection, the homogeneous phase immunoassay POCT detection technique overcomes the problem that CV is relatively high due to an NC membrane, so that the detection precision is high, generally the CV can be controlled to be within 5%, and the level of precision of the POCT detection technique can reach or even exceed that of the chemiluminescence immune assay technique. In the system using the homogeneous phase immunoassay POCT detection technique, a reagent card and a POCT analyzer are separately designed, a sample to be tested is acquired by using the reagent card, and the system is convenient to carry over.

The invention aims to provide a multiple PCR detection kit and a method for identifying susceptible bacteria species for intracranial post-operation to eliminate endogenous nucleic acid contamination. The detected bacteria species are the following common bacteria: staphylococcus aureus, enterococcus faecalis, streptococcus pneumoniae, escherichia coli, bacillus pyocyaneus, klebsiella pneumoniae and acinetobacter baumannii. Meanwhile, the invention provides the method to eliminate the endogenous nucleic acid contamination in a raw material of a PCR system and exclude a false positive experimental result of a PCR caused by the endogenous nucleic acid contamination. The detecting method using the kit has the advantages of that the operation is simple and fast, the detection cost is lowered, the detection result is accurate, and the false positive result is reduced. A negative control and a positive control are added in the amplification step, so that the judgment of false positive and false negative is effectively improved, and the detection accuracy is higher.

The invention relates to a double-labeled immunodetection method containing an internal reference, which is used for detecting a target protein in a sample. The method comprises the following steps of labeling a second binding protein of a target protein and an internal reference quality controller by adopting different markers, and substituting a marker signal value into a correction formula established by an inventor for calculation; substituting the calculation result into a fitting curve established based on the standard substance, and reversely solving the target protein concentration of the actual sample. The method can effectively reduce a problem of poor precision caused by the influence of interference factors on the detection result of the immunodetection reagent so that detection precision can be improved to CV less than 5%; based on the method, the inventor also designs a double-labeling kit for detecting troponin I, the influence of interference factors on a detection result can be effectively reduced, so detection precision is improved, and a powerful guarantee is provided for improving detection accuracy of existing immune quantitative analysis.

The invention relates to a multi-point-location on-line automatic collection system for gas in a livestock and poultry field area. The automatic collection system comprises a plurality of groups of gas production heads, a plurality of groups of gas inlet pipelines, a gas collection box and a gas backwashing system. Gas samples at all monitoring point locations of a farm enter the gas inlet pipelines through the gas production heads arranged at the top end of a telescopic supporting frame and then are conveyed into the gas collection box through the gas inlet pipelines, and the collected gas samples are switched in a gas sampling box through a gas inlet switching valve and then sequentially enter a gas monitoring instrument. According to a multi-point-location on-line automatic collection method for gas in a livestock and poultry field area, automatic online continuous collection of gas samples at a plurality of monitoring point locations in a livestock and poultry farm can be realizedautomatically and real-time continuous measurement of a gas on-line monitoring instrument can be realized. Compared with a conventional method, the method has the advantages that the gas collection process of the livestock and poultry farm is simplified, labor is saved, and the gas detection precision is improved.

The invention discloses a kit for quantitative detection of H-FABP and a H-FABP quantitative detection method. The kit for the quantitative detection of the H-FABP comprises a reagent strip, wherein aplurality of accommodating groove bodies are formed in the reagent strip; and the kit further comprises a magnetic particle reagent, a labeling reagent, a cleaning solution and a luminous substrate of alkaline phosphatase which are respectively pre-stored in different accommodating tank bodies, the magnetic particle reagent is prepared from magnetic beads coated with an anti-H-FABP primary antibody, and the labeling reagent is prepared from an anti-H-FABP secondary antibody subjected to activation treatment and alkaline phosphatase subjected to activation treatment. When the kit is used for detection, the operation is simple, the result is fast, the detection result is accurate, and the detection precision is high.

The invention relates to a method for detecting parecoxib sodium genotoxic impurities. The detection method comprises the following steps of dissolving an R1-M reference substance, an R1-C reference substance and an R1-N reference substance separately to obtain three kinds of impurity reference substance solutions; dissolving parecoxib sodium to be tested to obtain a sample solution to be tested;performing liquid chromatography/mass spectrometry determination on the three kinds of impurity reference substance solutions and the sample solution to be tested; the chromatographic conditions include: the mobile phase A is volatile acid or buffer solution, and the mobile phase B is acetonitrile; and the mass spectrometry conditions include: selecting an electrospray ionization, a positive ion scanning mode, the declustering potential is 25 to 200V, the collision energy is 10 to 100eV, the capillary voltage is 2000 to 6000V, the capillary temperature is 250 to 400 DEG C, and the drying gas temperature is 250 to 600 DEG C. The detection limit of the above method reaches 0.3ppm, the limit of quantification reaches 1ppm, and the quality control of pareximab sodium bulk drugs can be well carried out.

The invention discloses an analysis method of phosphorus content in phosphoric pig iron, and relates to the technical field of component analysis of pig iron. According to the method, a method utilizing spark source atomic emission spectrometer is used for detecting, so that the relative standard deviation is small, and the accuracy is high; compared with an inductively coupled plasma atomic emission spectrometry with high accuracy, the method has the advantages that the accuracy in detecting phosphorus content in phosphoric pig iron is high; and the requirement of detection on outsourced pigiron is met.

The invention relates to the technical field of chemiluminiscence detection, and particularly discloses a receptor reagent for homogeneous chemiluminiscence detection and application of the receptor reagent. The receptor reagent comprises receptor particles capable of reacting with active oxygen to generate a detectable chemiluminiscence signal, wherein the particle size distribution variable coefficient C.V value of the receptor particles in the receptor reagent is greater than or equal to 5%. Compared with the prior art, the performance of a kit of the receptor reagent is greatly improved, and the kit not only has ultrahigh sensitivity, but also has a very wide detection range.

The invention discloses a vacuum tank detection anti-explosion device, and belongs to a plastic tank pressure detecting device. The vacuum tank detection anti-explosion device comprises a box frame, avacuum tank fixing device and a pneumatic pump. The vacuum tank fixing device is fixedly installed on the box frame. The pneumatic pump is arranged in an inner cavity of the box frame. During working, a vacuum tank is fixed by the vacuum tank fixing device, a joint of the pneumatic pump is connected with an air inlet hole of the vacuum tank, so that a pressure test of the vacuum tank is realizedby the pneumatic pump. According to the vacuum tank detection anti-explosion device, the technical problem that an existing safety detection and protection device is high in the cost, complex in the structure and not suitable for production needs of small and medium sized car enterprises and part manufacturers are solved. Compared with an existing air pressure anti-explosion detection device, thevacuum tank detection anti-explosion device has the characteristics of simple structure, convenient operation, high detection safety performance, high detection precision, and simultaneous detection of two kinds of testing items including positive pressure and negative pressure, and at the same time, an inspector can observe test results at a close range, and popularization and use for the small and medium sized part manufacturers are facilitated.

The invention belongs to the research fields of plant growth and development, molecular heredity, cytobiology and the like, and relates to a plant hormone quantitative detection kit, a detection method and application. The plant hormone quantitative detection kit is used for accurately and quantitatively detecting 10 plant hormones in a plant sample. The plant hormone quantitative detection kit disclosed by the invention is small in required sample volume, simple in sample pretreatment, strong in specificity and matrix interference resistance, short in detection time, high in flux, high in detection precision and low in cost. The invention also discloses a method for quantitatively detecting plant hormones by using the kit.

The invention provides an automatic mobile phone middle plate detection device. The automatic mobile phone middle plate detection device is arranged on a conveyor belt of a mobile phone middle plate,wherein supporting frames are arranged on the two sides of the conveyor belt, an industrial computer, a vidicon and an illumination lamp are arranged on the supporting frames, and the industrial computer has a main control CPU and a display screen; the conveyor belt transmits a mobile phone middle plate to be detected, the illumination lamp illuminates the mobile phone middle plate, the camera ofthe vidicon is aligned with the mobile phone middle plate for photographing, the vidicon is in signal connection with the main control CPU to transmitting a photographed image to the main control CPU,and the main control CPU receives the image and compares the image with a standard image stored on the main control CPU so as to pick out unqualified products.

The invention discloses a receptor reagent and application thereof. The receptor reagent comprises a first buffer solution and receptor particles which suspend in the first buffer solution and can react with active oxygen to generate chemiluminescence signals, wherein the surfaces of the receptor particles are combined with biomolecules, and a particle size distribution variable coefficient C.V value of the receptor particles in the receptor reagent is not lower than 5% and not higher than 20%; and the sugar content in each milligram of the receptor particles is not higher than 40 [mu] g. The receptor reagent has the beneficial effects that the particle size distribution variable coefficient C.V value of the receptor particles in the receptor reagent is not lower than 5% and not higher than 20%; furthermore, the receptor reagent not only can meet the commercial requirement of mass production, but also has ultrahigh sensitivity and very wide detection range. Meanwhile, the sugar content in the receptor particles is controlled, so that the influence of sugar on a detection signal is reduced, the detection precision is improved, and the production cost is further reduced.

The invention relates to the technical field of immunological detection, in particular to a method for detecting DNASE1L3 based on a magnetic particle chemiluminescence immunoassay. The method comprises the following steps: preparing a biotinylated anti-DNASE1L3 antibody; preparing an enzyme-labeled anti-DNASE1L3 antibody; combining the biotinylated anti-DNASE1L3 antibody with the anti-DNASE1L3 antibody in a sample to be detected; adding streptavidin-coated magnetic particles, reacting, washing under the action of magnetic force, and removing uncombined substances; adding an enzyme-labeled anti-DNASE1L3 antibody, reacting, washing under the action of magnetic force, and removing uncombined substances; adding a buffer solution and a luminous substrate solution for reaction, detecting the luminous intensity on a luminoscope, and calculating the concentration of the DNASE1L3 in the sample. The preparation method and application of the mortise and tenon joint structure g-c3n4moo3 compositephotocatalytic material can rapidly realize quantitative analysis of DNASE1L3, and have the advantages of high detection sensitivity and strong specificity.

The invention provides an SDF-1 determination kit. The SDF-1 determination kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises 10-100 mmol/L of a buffer solution, 0.05-1 g/L of a preservative, 30-50 g/L of a coagulant, 5-15 g/L of a stabilizer and 2-5 mL/L of a surfactant; the reagent R2 comprises 40-100 mmol/L of a buffer solution, 1-2 g/L of a preservative, 0.5-1 g/L of a stabilizer, 2-5 mL/L of a surfactant and 30-50 mg/L of anti-human SDF-1 antibody mixed latex particles; wherein latex particles in the anti-human SDF-1 antibody mixed latex particles adopt two different particle sizes. The invention also provides a preparation method and a determination method of the SDF-1 determination kit. The kit is used for measuring the content of SDF-1, is simple and convenient to operate, short in detection time and low in cost, and also has relatively high sensitivity, precision and stability.