Electrochemical detection test paper of triglyceride and preparation method and application thereof, and detection sensor with the same

Abstract

The invention provides electrochemical detection test paper of triglyceride and a preparation method and application thereof, and a detection sensor with the same. The electrochemical detection test paper comprises an ink layer and a reaction enzyme layer; and the composition raw materials of the ink layer comprise carbon ink, an electronic mediator and a carbon nanotube. According to the providedelectrochemical detection test paper, in actual testing, the current gradient change is great, the precision is high, storing at room temperature can be achieved, and the stability is good.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biochemical detection, and relates to an electrochemical detection test paper for triglyceride, a preparation method and application thereof, and a detection sensor containing the same. Background technique [0002] The electrochemical test strip detection method can obtain various chemical and biological information of the tested sample in a short time with a small amount of blood sample. As a screening test, it is widely used in medical examination, family self-test and so on. For example, using electrochemical test strips to detect glucose and uric acid content in blood samples. [0003] Triglyceride is the most abundant lipid in the human body. Most tissues can use triglyceride decomposition products to supply energy. At the same time, tissues such as the liver can also synthesize triglyceride and store it in adipose tissue. As a kind of hyperlipidemia, high triglycerides mainly caus...

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Problems solved by technology

[0005] CN109655508A discloses a preparation method and application of an electrochemical sensor for detecting triglycerides; the sensor is prepared by fixing nitrogen-doped graphene on a glassy carbon electrode, and the patent uses immobilized lipase to directly Using lipase to detect triglycerides, the test sensitivity is poor

The reagent mixture used for the reagent coating includes surfactants, triglyceride hydrolysis reagents, glycerol kinase, glycerol-3-phosphate dehydrogenase, coenzymes, and redox reagents capable of being oxidized or reduced to form products; although the patent provides The test strip can realize the detection of triglycerides, but the electronic medium used is potassium ferricyanide, because lipoprotein lipase and surfactant can lyse red blood cells, release hemoglobin in the blood sample, and cause ferricyanide Potassium can react with ferrohemoglobin to generate potassium ferrocyanide and methemoglobin. The generation of potassium ferrocyanide makes the test background current too high, resulting in a relatively small current gradient when detecting triglycerides, that is, potassium ferricyanide Not suitable for electrochemical detection of triglycerides

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