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Homogeneous phase immunoassay POCT detection technique and system using same

A detection technology and immunoassay technology, applied in the field of homogeneous immunoassay POCT detection technology and systems using the technology, can solve the problems of low detection sensitivity, insufficient response, and small sample demand.

Inactive Publication Date: 2016-07-13
成都爱兴生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] F. The use of new technologies such as microfluidic chip type POCT detection technology has the advantages of fast response speed and small sample demand, but also due to insufficient response, resulting in low detection sensitivity.

Method used

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  • Homogeneous phase immunoassay POCT detection technique and system using same
  • Homogeneous phase immunoassay POCT detection technique and system using same
  • Homogeneous phase immunoassay POCT detection technique and system using same

Examples

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Effect test

Embodiment 1

[0083] Such as figure 1As shown, the present invention provides a homogeneous immunoassay POCT detection technology, comprising the following steps:

[0084] A. Mix the sample to be tested with the oxygen-supplying microsphere reagent; then mix it with the oxygen-receiving microsphere reagent;

[0085] B, the mixture in step A is irradiated with laser light, and the light intensity emitted by the mixture is measured simultaneously;

[0086] C. Calculate the concentration of the sample to be tested according to the light intensity;

[0087] The oxygen-supply microsphere reagent includes oxygen-supply microspheres, and the oxygen-accepting microsphere reagent includes oxygen-accepting microspheres, both of the oxygen-supplying microspheres and the oxygen-accepting microspheres are polystyrene microspheres, and the oxygen-supplying microspheres The surface of the ball is coated with hydrophilic aldehyde dextran, and the surface of the oxygen-receiving microsphere is coated with...

Embodiment 2

[0092] On the basis of Example 1, in step A, the sample to be tested is firstly diluted with a diluent, and then mixed with the oxygen-supplying microsphere reagent.

[0093] The diluent includes buffer, protein, stabilizer, preservative and the like. The diluent has the functions of dilution and buffering, which improves the accuracy of the final detection result and the stability of the sample to be tested.

Embodiment 3

[0095] When some specific detection methods are used, in addition to the sample to be tested, the oxygen-supplying microsphere reagent and the oxygen-receiving microsphere reagent, additional reagents are needed to carry out smoothly or can be optimized. In embodiment 1 or embodiment 2, in step A, first mix the sample to be tested with the first reagent evenly, and then mix it with the oxygen-supplying microsphere reagent.

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Abstract

The invention provides a homogeneous phase immunoassay POCT detection technique and a system using same. The homogeneous phase immunoassay POCT detection technique has the characteristics of high sensitivity, high precision and wide range of chemiluminescence immune assay technique and rapidness, portability and the like of a POCT detection technique; in addition, as pure liquid phase detection, the homogeneous phase immunoassay POCT detection technique overcomes the problem that CV is relatively high due to an NC membrane, so that the detection precision is high, generally the CV can be controlled to be within 5%, and the level of precision of the POCT detection technique can reach or even exceed that of the chemiluminescence immune assay technique. In the system using the homogeneous phase immunoassay POCT detection technique, a reagent card and a POCT analyzer are separately designed, a sample to be tested is acquired by using the reagent card, and the system is convenient to carry over.

Description

technical field [0001] The invention belongs to the field of immune analysis, and in particular relates to a homogeneous immune analysis POCT detection technology and a system using the technology. Background technique [0002] The existing chemiluminescent immunoassay technology is the mainstream technology for clinical testing and analysis, and is popular for its high sensitivity and wide analysis range. There are mainly the following disadvantages: [0003] A. The instrument system is bulky and occupies a large area. At the same time, due to the large test throughput, the reagent card adopts 100 tests as the whole unit, which requires the laboratory sample size; [0004] B. The instrument system and reagents are expensive and the maintenance cost is high, so it is not suitable for primary medical institutions; [0005] C. The instrument is bulky and cannot be carried into the diagnosis and treatment site; [0006] D. The chemiluminescent system mainly uses serum and pl...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/53G01N33/577
CPCG01N21/6402G01N33/5302G01N33/577
Inventor 包德泉
Owner 成都爱兴生物科技有限公司
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