Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for quantitative detection of H-FABP and H-FABP quantitative detection method

A H-FABP, quantitative detection technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of high detection cost, time-consuming, poor precision and sensitivity, etc., to improve detection efficiency and accurate detection results , The effect of high detection precision

Pending Publication Date: 2020-06-16
苏州翊讯生物科技有限公司
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the latex-enhanced immunoturbidimetric method is currently the most widely used because of its low cost and short detection time, but its precision and sensitivity are poor
However, other detection methods have the defects of high detection cost and long time consumption, and require high professionalism of operators

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for quantitative detection of H-FABP and H-FABP quantitative detection method
  • Kit for quantitative detection of H-FABP and H-FABP quantitative detection method
  • Kit for quantitative detection of H-FABP and H-FABP quantitative detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: Preparation of magnetic particle reagent

[0038] The magnetic particle reagent of the present invention is prepared as follows: mix 1 mg anti-H-FABP primary antibody with 100 mg MS-300 magnetic beads and 10 mg EDC, incubate at 37°C for 2 hours, collect magnetism, remove supernatant, add blocking agent CE210 , incubate at room temperature for 1H, collect magnetism, remove supernatant, add 1ml of magnetic bead preservation solution to prepare semi-finished magnetic particle reagent. Mix the prepared magnetic particle reagent semi-finished product with the magnetic bead buffer at a volume ratio of 1:50 to prepare the magnetic particle reagent. The magnetic beads in this embodiment are carboxyl-modified magnetic beads, which may also be other modified magnetic beads such as amino magnetic beads, hydroxyl magnetic beads, or mercapto magnetic beads.

[0039] The formula of the magnetic bead buffer of the present invention is: 4.58g / L TRIS, 6.81g / L NaCl, 0.1% T...

Embodiment 2

[0040] Example 2: Activation of Anti-H-FABP Secondary Antibody

[0041] Dissolve the activator 2-IT in a phosphate buffer solution with pH 7.2 to prepare a 2-IT solution with a concentration of 1.367 mg / ml, mix 2-IT and anti-H-FABP secondary antibody at a mass ratio of 1:50, After fully activating the reaction for 15 minutes, 2% by mass glycine of the anti-H-FABP secondary antibody was added to terminate the activation reaction, and then, excess 2-IT was removed by desalting using a PD10 column.

Embodiment 3

[0042] Example 3: Activation of Alkaline Phosphatase

[0043] Use DMF to prepare SMCC reagent with a concentration of 1.367mg / ml, mix SMCC reagent and alkaline phosphatase at a mass ratio of 1:50, mix well, activate the reaction for 15 minutes, and add 2% by mass of alkaline phosphatase to terminate the reaction Activate the reaction and remove excess SMCC by desalting using a PD10 column.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for quantitative detection of H-FABP and a H-FABP quantitative detection method. The kit for the quantitative detection of the H-FABP comprises a reagent strip, wherein aplurality of accommodating groove bodies are formed in the reagent strip; and the kit further comprises a magnetic particle reagent, a labeling reagent, a cleaning solution and a luminous substrate of alkaline phosphatase which are respectively pre-stored in different accommodating tank bodies, the magnetic particle reagent is prepared from magnetic beads coated with an anti-H-FABP primary antibody, and the labeling reagent is prepared from an anti-H-FABP secondary antibody subjected to activation treatment and alkaline phosphatase subjected to activation treatment. When the kit is used for detection, the operation is simple, the result is fast, the detection result is accurate, and the detection precision is high.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit for quantitative detection of H-FABP and a method for quantitative detection of H-FABP. Background technique [0002] In recent years, the incidence of acute coronary syndrome has been increasing, and its condition develops rapidly. If it cannot be treated in time, it will bring life-threatening to patients. In my country, the incidence of acute coronary syndrome is about 50 / 100,000. According to statistics, the number of people who die of acute myocardial infarction in my country exceeds 1 million every year. [0003] The duration of the diagnosis of acute coronary syndrome is directly related to the treatment effect. At present, the biochemical indicators used for acute coronary syndrome mainly include myoglobin, troponin and creatine kinase isoenzyme. However, myoglobin has a short half-life and is prone to false positive test results. The detection time ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/543
CPCG01N33/6893G01N33/581G01N33/54326G01N2800/324G01N2333/47
Inventor 江运强
Owner 苏州翊讯生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com