Method for measuring D-sorbitol in plasma or urine

A technology of sorbitol and plasma, applied in the field of drug analysis, can solve the problems of cumbersome operation, inaccurate quantification, and low sensitivity, and achieve the effects of high sensitivity, fast method, and easy operation

Inactive Publication Date: 2012-08-01
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the method for the determination of D-sorbitol in biological samples is mainly enzymatic spectrophotometry, which has defects such as low sensitivity, inaccurate quantification, and cumbersome operation.

Method used

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  • Method for measuring D-sorbitol in plasma or urine
  • Method for measuring D-sorbitol in plasma or urine
  • Method for measuring D-sorbitol in plasma or urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the mensuration of D-sorbitol concentration in canine plasma

[0030] 1. Experimental materials and instruments

[0031] D-Sorbitol reference substance: purchased from China Institute for the Control of Pharmaceutical and Biological Products; batch number: 100555-200306 Tinidazole reference substance (internal standard): purchased from China Institute for the Control of Pharmaceutical and Biological Products; batch number: 100336-200402 Test water: ultrapure Water; Methanol: chromatographically pure (Merck Company).

[0032] ZQ2000LC / MS liquid-mass spectrometry instrument (Waters Company of the United States, equipped with high-performance liquid phase instrument Waters 2695); Masslynx 4.0 data processing system; WH-2 micro-vortex mixer (Shanghai Huxi Analytical Instrument Factory); AE240 electronic balance ( Shanghai Mettler-Toledo Co., Ltd.); Millipore Drict-Q5 pure water machine (Millipore, France); Biofuge PrimoR refrigerated high-speed centrifuge (He...

Embodiment 2

[0051] The D-sorbitol content in plasma of the above three dogs given D-sorbitol was 70.0, 60.2, 65.4 μg·mL respectively -1 . Embodiment 2: Determination of D-sorbitol concentration in dog urine

[0052] 1. Experimental materials and instruments

[0053] D-Sorbitol reference substance: purchased from China National Institute for the Control of Pharmaceutical and Biological Products; batch number: 100555-200306 Tinidazole reference substance (internal standard): purchased from China National Institute for the Control of Pharmaceutical and Biological Products; batch number: 100336-200402 Test water: Super Pure water; methanol: chromatographically pure (Merck Company).

[0054] ZQ2000LC / MS liquid-mass spectrometry instrument (Waters Company of the United States, equipped with high-performance liquid phase instrument Waters 2695); Masslynx 4.0 data processing system; WH-2 micro-vortex mixer (Shanghai Huxi Analytical Instrument Factory); AE240 electronic balance ( Shanghai Mettl...

Embodiment 3

[0074] Embodiment 3: the mensuration of D-sorbitol concentration in canine plasma

[0075] With reference to Example 1, 3 dogs were continuously infused with 5% D-sorbitol solution intravenously for 3 hours, and the dosage was 150 mg·kg -1 . 3 hours after the infusion, 3ml of peripheral venous blood was collected, injected into a heparin tube for testing, and plasma was prepared. During sample processing, acetonitrile protein precipitation agent was used for protein precipitation. The rest of the conditions were the same, and the results were determined. The content of D-sorbitol in dog plasma was 53.1, 88.2, 54.4 μg·mL -1 .

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Abstract

The invention discloses a method for measuring D-sorbitol in plasma or urine with the liquid chromatography-mass spectrometry, which comprises the following steps: taking a to-be-tested sample, adding a certain amount of an organic solvent protein precipitation for protein precipitation, separating via a chromatographic column after pretreatment, and detecting with a mass spectrometric detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and convenient in operation, and is suitable for measuring concentration of D-sorbitol in plasma and urine.

Description

technical field [0001] The invention relates to a drug analysis technology, which relates to a method for analyzing and measuring drugs in vivo, in particular to a method for measuring the concentration of D-sorbitol in blood plasma or urine. Background technique [0002] Hepatic functional blood flow refers to the part of hepatic blood flow that flows through the hepatic sinusoid and exerts its function. Currently, it can only be measured by the method of clearance rate. Clinically, the clearance rate of D-sorbitol can reflect the total hepatic blood flow, and its change can reliably reflect the change of hepatic blood flow, thus reflecting the liver reserve function. Therefore, the hepatic clearance rate of D-sorbitol is a reliable indicator for choosing a treatment method and evaluating the therapeutic effect in the treatment of liver cirrhosis. The overall clearance rate and renal clearance rate of sorbitol can be calculated by measuring the plasma concentration and uri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 许美娟储继红刘史佳居文政张军吴婷李长印
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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