2 results about "Sorbitol solution" patented technology

The present application provides a kind of sorbitol continuous dehydration preparation isosorbide method, belong to the preparation field of bio-based chemicals. Using solvent containing dimethyl sulfoxide, sulfolane and other compounds to dissolve sorbitol, increase the mobility of sorbitol solution, and then realize the continuous dehydration of sorbitol to prepare isosorbide. The specific steps are as follows: the solid acid catalyst is loaded into the fixed bed reactor, the preheated sorbitol solution is continuously passed through the catalyst layer for dehydration reaction, and the mixture after reaction is separated to obtain isosorbide. The present application adds polar aprotic solvent to improve the flowability of the reaction solution during the dehydration reaction stage, realizes continuous reaction, and the dehydration reaction temperature is low, and the generated pyrolysis black substance is greatly reduced; aromatic hydrocarbon is used to extract high-boiling organic solvent in the product separation stage, and the separation effect is good and the energy consumption is low; the method has important application prospect.

This invention discloses a method for establishing a high-efficiency regeneration system for *Pyrus pyrifolia* leaves, belonging to the field of plant tissue culture technology. The method includes: spreading a layer of sorbitol solution on the surface of a culture medium; inoculating *Pyrus pyrifolia* seedlings into the medium and placing them in a dark environment for etiolation treatment; taking etiolated leaves from the *Pyrus pyrifolia* seedlings and inoculating them in a regeneration medium for dark culture, then transferring them to light for further culture to produce adventitious buds; after the adventitious buds reach the target length, cutting off their lateral buds and placing them on a subculture medium to obtain regenerated seedlings. This invention, by adding sorbitol solution before dark treatment, can significantly shorten the time to obtain etiolated material to 7 days, and the etiolated plants are in better condition after three weeks; furthermore, by placing *Pyrus pyrifolia* tissue culture seedlings in a dark environment for dark culture, the physiological state of the tissue culture seedlings is altered, and the obtained etiolated leaves are inoculated into the regeneration medium, improving the regeneration efficiency, and the material acquisition is not affected by the season.