Flos lonicerae and radix scutellariae lung-clearing preparation fingerprint spectrum establishment method and standard fingerprint spectrum and application thereof

A standard fingerprint, the technology of Yinhuang Qingfei, is applied in the application field of the fingerprint in the quality control of the preparation, and can solve the problems of not being able to better guarantee the clinical efficacy and the like

Active Publication Date: 2015-07-22
湖南安邦制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Yinhuang lung-clearing preparation is mainly used for clearing the lungs and resolving phlegm, relieving cough and relieving asthma. There is no good quality control method in its existing quality standards and other prior art to fully and accurately control product quality, and it cannot be guaranteed better. Clinical efficacy

Method used

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  • Flos lonicerae and radix scutellariae lung-clearing preparation fingerprint spectrum establishment method and standard fingerprint spectrum and application thereof
  • Flos lonicerae and radix scutellariae lung-clearing preparation fingerprint spectrum establishment method and standard fingerprint spectrum and application thereof
  • Flos lonicerae and radix scutellariae lung-clearing preparation fingerprint spectrum establishment method and standard fingerprint spectrum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0165] Example 1 Establishment of Yinhuang Qingfei Capsule Fingerprint

[0166] (1) Instruments and reagents

[0167] Shimadzu LC-10ATVP high performance liquid chromatograph, CLASS-VP 5.0 chromatographic workstation; Inertil C18 chromatographic column.

[0168] (2) preparation of need testing solution

[0169] Take about 1.2 grams of the contents of 130201 batches of Yinhuang Qingfei Capsules, add 30ml of methanol precisely, weigh, and ultrasonically treat for 30 minutes. After cooling, use methanol to make up for the lost weight, filter through a microporous membrane, and take the subsequent filtrate to obtain .

[0170] (3) Preparation of reference substance solution

[0171] Take an appropriate amount of ephedrine hydrochloride reference substance, add methanol to make a solution containing 0.2mg per 1mL, and obtain it;

[0172] Take an appropriate amount of schisandra alcohol A reference substance, add methanol to make a solution containing 6mg per 1mL, and obtain it;...

Embodiment 2

[0209] Example 2 Establishment of Fingerprint of Yinhuang Qingfei Capsules

[0210] (1) Instruments and reagents

[0211] Shimadzu LC-10ATVP high performance liquid chromatograph, CLASS-VP 5.0 chromatographic workstation; Inertil C18 chromatographic column.

[0212] (2) preparation of need testing solution

[0213] Take about 1.5 grams of the contents of 130104 batches of Yinhuang Qingfei capsules, accurately add 40ml of methanol, accurately weigh, reflux and extract for 30 minutes, make up the lost weight with methanol after cooling, filter through a microporous membrane, and take the subsequent filtrate, namely have to.

[0214] (3) Preparation of reference substance solution

[0215] Take an appropriate amount of ephedrine hydrochloride reference substance, add methanol to make a solution containing 0.2mg per 1mL, and obtain it;

[0216] Take an appropriate amount of schisandra alcohol A reference substance, add methanol to make a solution containing 6mg per 1mL, and ob...

Embodiment 3

[0252] Example 3 Establishment of Fingerprint of Yinhuang Qingfei Capsules

[0253] (1) Instruments and reagents

[0254] Shimadzu LC-10ATVP high performance liquid chromatograph, CLASS-VP 5.0 chromatographic workstation; Inertil C18 chromatographic column.

[0255] (2) preparation of need testing solution

[0256] Take about 1 g of the contents of 130216 batches of Yinhuang Qingfei capsules, accurately add 50ml of methanol, weigh, ultrasonically treat for 60 minutes, make up for the lost weight with methanol after cooling, filter through a microporous membrane, and take the subsequent filtrate to obtain .

[0257] (3) Preparation of reference substance solution

[0258] Take an appropriate amount of ephedrine hydrochloride reference substance, add methanol to make a solution containing 0.2mg per 1mL, and obtain it;

[0259] Take an appropriate amount of schisandra alcohol A reference substance, add methanol to make a solution containing 6mg per 1mL, and obtain it;

[026...

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Abstract

The invention discloses a flos lonicerae and radix scutellariae lung-clearing preparation fingerprint spectrum establishment method and a standard fingerprint spectrum and application thereof. According to the method, pretreatment methods of various test samples are simple, characteristic ingredients can be retained completely, product quality information can be comprehensively reflected, the fingerprint spectrum has strong specificity and stability, and high precision, separation effect of each characteristic peak in the obtained fingerprint spectrum is good; the fingerprint spectrum can be used to quickly determine whether a flos lonicerae and radix scutellariae lung-clearing preparation is qualified so as to ensure the stable quality and the clinical curative effect of the flos lonicerae and radix scutellariae lung-clearing preparation.

Description

technical field [0001] The invention relates to a method for establishing a fingerprint of a traditional Chinese medicine preparation and a standard fingerprint thereof, and also relates to the application of the fingerprint in the quality control of the preparation. Background technique [0002] Yinhuang lung-clearing preparation is mainly used for clearing the lungs and resolving phlegm, relieving cough and relieving asthma. There is no good quality control method in its existing quality standards and other prior art to fully and accurately control product quality, and it cannot be guaranteed better. Clinical efficacy. Therefore, in order to control the quality of the product more comprehensively and effectively, it is very necessary to establish its fingerprint for quality control. Contents of the invention [0003] An object of the present invention is to provide a method for establishing a fingerprint of Yinhuang Qingfei preparation based on high performance liquid c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张水寒高尚周卿意骏
Owner 湖南安邦制药股份有限公司
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