302 results about "Ion current" patented technology

A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units. In a further embodiment, different voltages are applied across the nanopore during translocation in order to improve the resolution of polymer units.

A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units. In a further embodiment, different voltages are applied across the nanopore during translocation in order to improve the resolution of polymer units.

The invention relates to the technical field of biology, in particular to a signal noise reduction processing method and system, and the method comprises the steps: collecting an original ion current signal, and constructing an original ion current signal segment and a training set associated with a pure ion current signal segment corresponding to the original ion current signal segment; constructing a noise reduction neural network model, and defining a loss function for the noise reduction neural network model; training trainable parameters of the noise reduction neural network model through the training set and the loss function so as to complete training of the noise reduction neural network; inputting all to-be-detected original ion current signal segments of to-be-detected original ion current signals into the trained noise reduction neural network model, and outputting corresponding to-be-detected noise reduction ion current signal segments; and splicing all the to-be-detected noise reduction ion current signal segments to obtain a complete to-be-detected noise reduction ion current signal. According to the invention, the noise in the original ion current signal can be effectively removed, and the distortion of the ion current signal is avoided, so that the recognition effect of the basic group is ensured.

Provided is a fluid injection device (10) capable of efficiently administering a drug. A first transport body (11) has a first transport flow path through which a first fluid (21) flows, and a second transport body (12) has a second transport flow path through which a second fluid (22) flows. Positive-side microneedles (13) provided to the first transport body (11) have a first opening and a first flow path to which a positive charge is fixed. Negative-side microneedles (14) provided to the second transport body (12) have a second opening and a second flow path to which a negative charge is fixed. When a current / voltage application means (15) applies a current or voltage between a first electrode (24) disposed in the first transport flow path and a second electrode (25) disposed in the second transport flow path, an ion current flows through the first fluid (21) and the second fluid (22), and the first fluid (21) flows from the first opening toward the outside and the second fluid (22) flows from the second opening toward the outside by electroosmotic flow.

A pulse generator is disclosed. The pulse generator includes a DC source; a plurality of switches, a transformer; and a pulsing output. The pulse generator can be coupled with a plasma chamber. The pulsing output outputs high voltage pulses having a peak-to-peak voltage greater than 1 kV and a voltage portion between consecutive high voltage bipolar pulses that has a negative slope that substantially offsets the voltage reduction on a wafer within a plasma chamber due to an ion current. The resulting voltage at the wafer may be substantially flat between consecutive pulses.

A sensor device (902) for a nanopore sensor, the sensor device (902) comprising: an insulating substrate (903); one or more wells (908) for containing a fluid, where the one or more wells (908) are formed on a first side of the substrate (903); a sensor electrode (910) for detecting an ionic current in each of the one or more wells (908); wherein the sensor electrode (910) is formed on the first side of the substrate (903) at the base of the one or more wells (908); one or more contacts (914) formed on a second side (903) of the substrate; and one or more through holes (912) extending through the substrate (903); wherein the one or more through holes (912) connect the sensor electrode (910) to the one or more contacts (914); wherein the electrode (910) comprises a different structure than the one or more contacts (914), and / or the sensor electrode (910) is made of a different material or materials than the one or more contacts (914).

A method of performing nucleic acid sequencing may comprise using a disintegrator situated in a fluidic channel of a sequencing device to cleave off a portion of a nucleic acid molecule in the fluidic channel; applying an electrostatic force to divert the portion of the nucleic acid molecule through a nanopore; detecting an ionic current through the nanopore; and determining an identity of at least one nucleotide of the portion of the nucleic acid molecule based at least in part on the ionic current. A system for sequencing nucleic acids may comprise an array comprising a plurality of sequencing devices, each comprising a fluidic channel, a disintegrator embedded in the fluidic channel, and a nanopore coupled to an exit end of the fluidic channel; and detection circuitry coupled to the array and configured to detect ionic currents through the nanopores.

A pulse generator is disclosed. The pulse generator includes a DC source; a plurality of switches, a transformer; and a pulsing output. The pulse generator can be coupled with a plasma chamber. The pulsing output outputs high voltage pulses having a peak-to-peak voltage greater than 1 kV and a voltage portion between consecutive high voltage bipolar pulses that has a negative slope that substantially offsets the voltage reduction on a wafer within a plasma chamber due to an ion current. The resulting voltage at the wafer may be substantially flat between consecutive pulses.

The invention discloses a cold cathode ionization gauge driving acquisition circuit, relates to the field of vacuum measurement, and solves the problems of signal conversion nonlinearity and large ion flow when a gauge tube works at a lower vacuum degree in the existing cold cathode ionization gauge driving acquisition technology. Comprising an ion current logarithmic acquisition circuit used for converting the ion current of the cold cathode ionization gauge into a voltage signal, a current monitoring circuit used for generating a control signal according to the voltage signal, and a voltage-controlled high-voltage driving circuit used for driving the cold cathode ionization gauge according to the control signal. The nonlinear compensation operation conditioning circuit is used for performing nonlinear compensation on the voltage signal according to the control signal to obtain an operation acquisition voltage, and the operation acquisition voltage is used for being input into an acquisition system; the non-linear problem of signal conversion is solved through the non-linear compensation circuit, negative feedback loop control is carried out through the current monitoring circuit and the voltage-controlled high-voltage driving circuit, the driving voltage of a gauge tube is reduced, and the maximum ion current is limited at a low level.

The invention provides a glass nanopore probe based on an aptamer and application of the glass nanopore probe in dopamine detection, and belongs to the technical field of electrochemical sensing and scanning probe microscopy. The sensor comprises: a glass nanopore probe, the inner wall of which is modified with a gold layer; a cDNA (complementary deoxyribonucleic acid) single chain is fixed on the gold layer through a gold-sulfur bond; a part of the sequence of the dopamine aptamer is hybridized and combined with the cDNA single chain; wherein the combination of the dopamine and the dopamine aptamer causes the change of an ion current rectification signal of the glass nanopore probe, so that the detection of the dopamine is realized based on the change of the ion current rectification signal of the glass nanopore probe. The invention provides a valuable scheme for developing an aptamer nanopore scanning electrochemical sensor for single cell analysis and researching neurotransmitter-related diseases.

The invention belongs to the field of scanning ion conductance microscopes and scanning electrochemical cell microscopes, and discloses a scanning imaging system and method based on optical feedback positioning and ion conductance technology, the scanning imaging system comprises an SICM ion current circuit, an optical path reflector and an adapter bracket, the adapter bracket is arranged on a motion platform, and a laser generator is arranged on the upper part of the adapter bracket; the light path reflector is rotationally connected with the adapter bracket, a prism system and a CCD camera are sequentially arranged on a reflected light path of the light path reflector, and the prism system is fixed on the adapter bracket; the lower part of the adapter bracket is provided with a micromotor, the output end of the micromotor is connected with a Z-direction piezoelectric ceramic, the Z-direction piezoelectric ceramic is connected with a piezoelectric ceramic controller and is connected with the flexible hinge, the Z-direction piezoelectric ceramic is provided with a probe holder, the SICM probe is arranged on the probe holder, and the probe electrode is arranged in the SICM probe. According to the invention, the position of the probe can be rapidly and accurately controlled, and the problem of low scanning speed of scanning ionic conductivity detection equipment is solved.

The invention relates to a single cell metabonomics-based environmental pollutant toxic effect analysis method, and belongs to the technical field of single cell mass spectrometry. The invention relates to a single-cell metabonomics-based environmental pollutant toxic effect analysis method. The method comprises the following steps: S1, importing mass spectrum flow type original data and extracting a target ion current diagram; s2, identifying a single cell mass spectrum signal and deducting a background signal; s3, matching and identifying metabolites; S4, carrying out data standardization processing and data quality control; S5, identifying differential metabolites; and S6, carrying out pathway enrichment analysis on the screened differential metabolites. According to the analysis method disclosed by the invention, high-quality treatment of single cell metabonomics data is realized, and high-throughput, multi-component and label-free analysis is carried out on pollutants in a single cell.

The invention discloses a waste gas non-methane total hydrocarbon emission continuous monitoring system and method, and relates to the related technical field of environmental monitoring, and the system comprises: a connection collection device, which is used for extracting to-be-detected gas from a waste gas source; carrying out gas chromatographic separation, and determining separated components; detecting through a hydrogen flame ionization detector to generate an ion current, and converting the ion current to obtain a current signal; weak current analysis is carried out, and a concentration-signal relation is established through fitting; extracting real-time to-be-detected gas for passing treatment, and generating real-time concentration in combination with the concentration-signal relationship; and the real-time concentration is output through a monitoring interface. The technical problem that in the prior art, continuous monitoring is difficult to conduct to effectively extract and separate different pollutant components, and consequently the accuracy and timeliness of the emission monitoring result are insufficient is solved, and the technical effect of improving the accuracy and timeliness of the emission monitoring result is achieved.

Embodiments of this disclosure generally relate to systems used in semiconductor device manufacturing processes. [Solution] Embodiments provided herein generally include apparatus and methods for synchronizing and controlling the supply of RF bias voltage signals and pulsed voltage waveforms to one or more electrodes inside a plasma processing chamber. Embodiments of the Disclosure include methods and apparatus for synchronizing a pulsed radio frequency (RF) waveform with a pulsed voltage (PV) waveform so that the pulsed RF waveform is turned on during a first stage of the PV waveform and turned off during a second stage. The first stage of the PV waveform includes a sheath collapse stage. The second stage of the PV waveform includes an ion current stage.

The invention relates to means and methods for analysis of target analytes using nanopore-based sensors, more in particular to methods, nanopore systems and devices for single-molecule profiling of polymers, e.g. polypeptide or polysaccharides. Provided is a method for translocating a non-nucleic acid based polymer analyte through a nanopore, the nanopore being comprised in a membrane separating a fluidic chamber of a nanopore system into a cis side and a trans side, comprising adding the analyte to the cis side of and allowing for translocation, wherein the nanopore system has a cis to trans electro-osmotic force (EOF) resulting from a net ionic current flow cis to trans, preferably wherein the cis to trans EOF results from a net ionic current flow cis to trans over total ionic current flow of greater than 0.2 or less than −0.2.

The invention discloses a method for detecting polypeptide sequence difference and post-translational modification thereof based on a nanopore system, the nanopore system is constructed in the method, and the nanopore system comprises an alpha-hemolysin nanopore and octa-amino modified gamma-cyclodextrin embedded in the nanopore; the amino acid sequence of the alpha-hemolysin nanopore comprises mutation sites M113R and T145R, and the alpha-hemolysin nanopore is characterized in that the alpha-hemolysin nanopore According to the detection method, polypeptide with negative charges enters a nanopore under the driving of external voltage, an ion current blocking signal is recorded, and the polypeptide sequence difference and post-translational modification are analyzed according to blocking amplitude, duration, noise characteristics and event frequency. The method can detect a peptide chain with the length of 2-10 amino acid residues, can distinguish the post-translational modification state in homopolypeptide composed of 4-10 amino acids and polypeptide by single amino acid resolution, and can be used for protein enzyme digestion product analysis, oligopeptide drug detection and rapid polypeptide identification under the condition of no mass spectrum.

The invention belongs to the technical field of beauty equipment, and particularly relates to a beauty light zirconium ion excitation device which comprises a device shell, the head of the device shell is provided with an ion excitation head, the ion excitation head comprises an insulating base and an excitation electrode group fixed on the insulating base, and the excitation electrode group is formed by arranging a plurality of microelectrodes in an array form; the device shell is internally provided with an ion excitation circuit, the ion excitation circuit comprises a high-voltage generation module and an electrode driving module, the high-voltage generation module is electrically connected with the electrode driving module, and the electrode driving module is respectively connected with each microelectrode in the excitation electrode group through a wire. According to the invention, the electrode group formed by arranging a plurality of independent microelectrodes in a specific array is arranged on the ion excitation head, the electrode driving module is respectively connected with the microelectrodes, and the array formed by the plurality of microelectrodes can physically form an ion excitation source with wider coverage and more dense distribution; and the generated ion current is milder and more stable.

A misfire detection system (300) is disclosed, comprising an ignition system (303) including an ignition coil (107), an ion current measuring circuit (302) electrically connected to the ignition coil (107) for measuring an ion current generated by a spark plug (106), and a signal processor (401) for processing and conditioning the ion current measured by the ion current measuring circuit (302) to determine the occurrence of a misfire in the IC engine. The ignition coil (107) comprises a primary winding (101) and a secondary winding (103), wherein a first end (101B) of the primary winding (101) is electrically connected to a first end (103B) of the secondary winding (103). Misfire detection improves the reliability and durability of the IC engine.

The invention relates to a method for pretreating a surface of containers (2) before printing, such as digital printing, wherein the method comprises: - Emitting a plasma stream by means of at least one plasma unit (7, 8) comprising at least one plasma source (9, 10, 11, 12, 13, 14) for emitting the plasma stream and arranged next to a transport path for containers, - Moving the containers along the transport route and rotating the containers about their longitudinal axis (5), - Irradiating at least part of the surface of the containers with at least part of the plasma stream. The invention further relates to a device (1) for pretreating a surface of containers (2) before printing, such as digital printing, with: - a transport and rotation device (3) for moving the containers along a transport path and for rotating the containers about their longitudinal axis (5), - at least one plasma unit (7, 8) comprising at least one plasma source (9, 10, 11, 12, 13, 14) for emitting a plasma stream, which is arranged next to the transport route for containers, and for irradiating at least a part of the surface of the containers with at least a part of the plasma stream.

This invention relates to a safety protection method and circuit for preventing electron backflow in an ion electric thruster, applicable to spacecraft ion electric propulsion systems. The ion electric thruster's grid gate is connected to the positive output terminal of the grid power module, the acceleration grid gate is connected to the negative output terminal of the acceleration power module, and the common ground is connected to the common power ground of both the grid power module and the acceleration power module. The safety protection method involves detecting the output current of the acceleration power module to determine if an overcurrent has occurred. If an overcurrent has occurred, a synchronous shutdown signal is generated and transmitted to the PWM controllers of 1 to N grid power modules and acceleration power modules. Upon receiving the synchronous shutdown signal, the PWM controllers of the 1 to N grid power modules and acceleration power modules synchronously shut down their voltage output terminals, thereby achieving synchronous 0V voltage signal output from the positive high-voltage output terminals of the 1 to N grid power modules and the negative high-voltage output terminal of the acceleration power module, preventing electron backflow caused by any grid module failing to shut down its high-voltage output.

This invention relates to a high-efficiency ion current acquisition structure and a mass spectrometer. The ion current acquisition structure includes: a collection chamber adapted to be installed between the ion source and the sampling cone of the mass spectrometer; a trumpet-shaped aperture disposed within the collection chamber at the end facing the ion source; an annular electrode disposed on the inner wall of the trumpet-shaped aperture; and a beam rod assembly comprising multiple rod-shaped electrodes uniformly arranged circumferentially along the outlet of the trumpet-shaped aperture, with adjacent rod-shaped electrodes spaced apart to form gaps; wherein, on the side away from the ion source, the ends of the multiple rod-shaped electrodes are all bent and converged towards the central axis of the collection chamber. This invention provides a high-efficiency ion current acquisition structure that can improve the technical problems of low ion acquisition efficiency before entering the vacuum system and high background noise caused by the inability to effectively filter neutral particles in the prior art.

This invention relates to the field of microscopy application technology, and in particular to a SICM scanning control method and related device based on feedback quantization. The method acquires the test height, initial scanning speed, initial safe height of the test point, first speed adjustment start point, and second speed adjustment start point of the test point. It controls the scanning probe to descend from the initial safe height of the test point to the first speed adjustment start point at the initial scanning speed, intervenes with first dynamic speed adjustment, continues to descend to the second speed adjustment start point, intervenes with second dynamic speed adjustment, and continues descending until the probe detects a sudden change in ion current and stops descending. This control method solves the problems of low scanning time resolution and poor safety in existing scanning ion conductivity microscopes by setting an initial speed adjustment point that dynamically adjusts the speed according to the probe's descent position.

The invention discloses a laser ion source system based on double pulses and AI feedback control and a control method, and relates to the technical field of plasmas, and the system is characterized in that a first laser generates pre-ablation laser, and generates pre-plasma on a target material; the second laser generates main ionization laser which irradiates the pre-plasma to generate main plasma; and the intelligent control unit extracts spatial features from the plume image, fuses the spatial features, the spectral data, the real-time ion current intensity and the equipment parameters to obtain high-order state features, and generates laser pulse optimization parameters according to the high-order state features and the equipment parameters. According to the invention, a double-pulse mechanism is adopted to enable main ionization to occur in the expanded plasma, charge exchange and energy dispersion are reduced, the energy divergence of the ion beam is remarkably reduced, the energy conversion efficiency is improved, AI real-time feedback control can compensate factors such as target pitting corrosion and laser power fluctuation, and very high inter-pulse stability is realized.

The invention discloses a multi-channel ion current detection circuit for an aero-engine pedestal. The multi-channel ion current detection circuit comprises a signal control box and a dual-channel real-time detection circuit, in each channel of the real-time detection circuit, the multi-gear ion current display circuit is connected to an ion current detection loop of the signal control box, and the ion current of the ion current detection loop in the signal control box is monitored in real time; in the threshold value display circuit and the multi-gear ion current threshold selection circuit which are connected in series, voltage division is realized through divider resistors which are connected in series at the front ends, adjustable resistors are connected in series on all voltage division paths, and all the voltage division paths which are connected in parallel and the adjustable resistors in the voltage division paths are connected to an ion current threshold value adjusting circuit of the signal control box. The ion current threshold value of the current threshold value adjusting circuit is set by adjusting the resistance value of the adjustable resistor in each voltage dividing path, and the voltage display of each voltage dividing resistor is realized through a voltmeter V1 connected with each voltage dividing resistor, so that the ion current threshold value set in the corresponding voltage dividing path is reflected through the display of the voltmeter V1. According to the technical scheme, the problems that an existing ionic current detection method cannot detect data of ionic current in an afterburner of an engine in real time, and the flame signal threshold value is difficult to adjust in the engine test run process are solved.

A pulsed ion current antenna includes an enclosed racetrack having an interior configured to be placed under vacuum. The enclosed racetrack has an ion injection zone, a beam merging zone, a first beam bending zone, a beam return zone, and a second beam bending zone. An ion source is provided at an end of the ion injection zone. Two parallel magnet plates are provided in each of the first and second beam bending zones, configured to produce a respective magnetic field that bends a path of travel of an ion beam within the enclosed racetrack. A plurality of loop coils are configured to generate magnetic fields to shape travel of ions within the enclosed racetrack such that ions from the ion source that are injected through the ion injection zone are merged in the beam merging zone into an ion beam within the enclosed racetrack.

A sensor device (902) for a nanopore sensor, the sensor device (902) comprising: an insulating substrate (903); one or more wells (908) for containing a fluid; wherein the one or more wells (908) are formed on a first side of the substrate (903); a sensor electrode (910) for detecting an ionic current in each of the one or more wells (908); wherein the sensor electrode (910) is formed on the first side of the substrate (903) at the base of the one or more wells (910); one or more through holes (912) extending through the substrate (903); wherein the one or more through holes (912) are connected to the sensor electrode (910); wherein the one or more through holes (912) each comprise a conductive cap (950) at one or both ends of the through hole (912).

The application discloses a cold cathode ionization gauge driving and collecting circuit, and relates to the field of vacuum measurement, which solves the problems of signal conversion nonlinearity and excessive ion current when the gauge tube works at a lower vacuum degree in the existing cold cathode ionization gauge driving and collecting technology, and comprises the following parts: an ion current logarithmic collecting circuit, which is used for converting the ion current of the cold cathode ionization gauge into a voltage signal; a current monitoring circuit, which is used for generating a control signal according to the voltage signal; a voltage-controlled high-voltage driving circuit, which is used for driving the cold cathode ionization gauge according to the control signal; a nonlinear compensation operation conditioning circuit, which is used for performing nonlinear compensation on the voltage signal according to the control signal to obtain an operation collecting voltage; and the operation collecting voltage is used for inputting into a collecting system; the problem of signal conversion nonlinearity is overcome through the nonlinear compensation circuit, and the negative feedback loop control is performed through the current monitoring circuit and the voltage-controlled high-voltage driving circuit, so that the driving voltage of the gauge tube is reduced, and the maximum ion current is limited at a lower level.

The invention relates to the technical field of plasma detection, in particular to a dual-channel ion energy probe based on a magnetic deflection effect. The invention provides a dual-channel ion energy probe based on a magnetic deflection effect. The dual-channel ion energy probe comprises an upper permanent magnet plate, a lower permanent magnet plate, a dual-channel collimator and a dual-channel probe, the dual-channel collimator penetrates into the shell and comprises a scanning channel and a reference channel, the scanning channel and the reference channel are connected with voltages of different values, the dual-channel collimator is used for enabling ions to enter the shell along the channel direction, and the upper permanent magnet plate and the lower permanent magnet plate are respectively fixed at the upper end and the lower end of the shell to form a uniform magnetic field. The dual-channel probe is arranged in the shell and comprises a scanning collection probe coplanar with the scanning channel and a reference collection probe coplanar with the reference channel, and the scanning collection probe and the reference collection probe are connected with voltages of different values respectively. According to the scheme, the ion current probe with a high signal-to-noise ratio can be provided.

The utility model provides an ion current skin repair instrument control system, which comprises a central control module electrically connected with a display module, and is characterized in that the central control module is electrically connected with an ion emitter, the ion emitter is electrically connected with a beauty control handle, the central control module comprises a controller, and the controller is electrically connected with a battery. A timing module, a charging module and an adjusting module are further installed on the central control module, and the controller is electrically connected with the timing module, the charging module and the adjusting module; the timing module is used for measuring a time interval in a beauty operation process; the charging module is used for managing and recording beauty fees; and the adjusting module is used for adjusting the working parameters of the ion emitter according to different beauty treatment requirements. According to the utility model, the beauty treatment accuracy and individuation are improved, and the beauty treatment effect is improved.

The application discloses a real-time monitoring method for a water heater controller system and relates to the technical field of water heater flame monitoring and control. The real-time monitoring method for the water heater controller system comprises the following steps: flame ion current signal monitoring, flame state identification process monitoring and flame regulation state monitoring. The application monitors the flame state detection state of the water heater controller system under different flame change speeds, then performs real-time monitoring and real-time analysis on the level input and output of the flame ion current signal, performs flame state identification early warning and signal strength interference judgment, finally performs interference analysis and judgment on the flame regulation process, realizes real-time capture, dynamic identification and anti-interference regulation closed-loop optimization of the flame ion current signal, and effectively solves the problem of inaccurate actual flame detection result caused by untimely capture of the flame ion current signal in the water heater controller system in the prior art.