The invention relates to the technical field of
microscopic imaging, and particularly provides a
stimulated Raman scattering
microscopic imaging system capable of simultaneously scanning double Raman
frequency shift spectrums, which comprises a
laser light source driving and modulating unit and a
signal detecting and
data processing unit, the
laser light source driving and modulating unit comprises a dual-channel
laser synchronous excitation module, a
light beam polarization regulation and power distribution module, a
pulse broadening and spectrum focusing module, a dual-channel dual-
phase modulation module, a common-path different-time dual-
frequency difference module and a
laser scanning and telecentric focusing module; and the
signal detection and
data processing unit comprises a dual-channel orthogonal
demodulation module and a scanning driving and
signal synchronous acquisition module. Compared with the prior art, the dual-Raman
frequency shift spectrum scanning
stimulated Raman microimaging device has the advantages that dual-Raman
frequency shift spectrum scanning
stimulated Raman microimaging with higher acquisition speed,
high resolution and wide spectrum coverage range is realized, and the problem of long time consumption for acquiring the complete spectrum of the to-be-tested sample by the traditional single-frequency
resonance spectrum scanning stimulated Raman microimaging is solved.