The invention provides a method for determining the position of a reflection interface of seismic data, and belongs to the field of petroleum geophysical exploration. The method includes: (1) supposing seismic waves as f (t), performing Hilbert transform of the f (t), calculating analytic signals of real seismic signals, and obtaining the instantaneous amplitude, the instantaneous phase section Theta (t), and the instantaneous frequency of the seismic waves f (t); (2) performing first-order differential operation based on the instantaneous phase section Theta (t) of the seismic waves, and obtaining the instantaneous phase first-order difference G (t); and (3) obtaining F (t)=-f (t) via the polarity reverse processing of the seismic data f (t), performing Hilbert transform of the F (t), obtaining the instantaneous phase section Theta1 (t), performing first-order differential operation of the instantaneous phase section Theta1 (t), obtaining the instantaneous phase first-order difference j (t) of the seismic waves after polarity reverse, and calculating J (t)=-j (t) via the calculation of the opposite number of the j (t).