The invention discloses a preparing method of Guizhou three-flavor paste particles. The method comprises the following steps: screening and cleaning ripe roxburgh rose fruits, airing surface moisture, and juicing; taking part of roxburgh rose juice for standby; separating roxburgh rose meat and roxburgh rose seeds after juicing, drying, shattering and grinding so as to obtain roxburgh rose mesh micro powder for standby, wherein the roxburgh rose mesh micro powder can pass through a sample separating screen with 300 meshes; sterilizing, shattering and grinding fagopyrum tararicum, so as to obtain fagopyrum tararicum micro powder which can pass through the sample separating screen with the 300 meshes; shattering cane sugar so as to enable the cane sugar powder to pass through a sample separating screen with 80 meshes, weighting the roxburgh rose meat micro powder, the fagopyrum tararicum micro powder, radix puerariae powder and the cane sugar powder according to a formula proportion, placing the powders in a stirring machine, uniformly mixing and stirring, adding the roxburgh rose juice and proper purified water, mixing, stirring, pelletizing, and then finishing so as to obtain cane sugar type particles; or placing maltodextrin, a composite sweetener, the roxburgh rose meat micro powder, the fagopyrum tararicum powder and the radix puerariae powder in the stirring machine, uniformly mixing and stirring, adding the roxburgh rose juice and the proper purified water, mixing, stirring, pelletizing, and then finishing so as to obtain lower sugar type particles.