Method for establishing polygonatum sibiricum red high-frequency regeneration system and culture medium

Abstract

The invention aims at solving the problems that seed propagation adopted in current polygonatum sibiricum red production is difficult, plant diseases and insect pests can easily become more serious and germplasm can be easily degenerated during the conventional rhizome propagation, tissue culture mainly takes terminal buds of rhizome and stem tips as explants for establishing a rapid propagation system, source of the explant is single, test material consumption is high and propagation efficiency is low. According to the method provided by the invention, easily available polygonatum kingianum terminal bud scales are selected as an explant test material, adventitious roots are obtained by inducing bud scale differentiation, and then further induction is carried out on the adventitious roots, so that differentiation bodies of different types such as rhizomes having adventitious roots and terminal buds at the same time, adventitious bud clumps and callus tissues in multiple forms are obtained; and respectively continuously culturing the differentiation bodies of different types, so that new adventitious bud clumps, rhizomes with terminal buds and callus tissues of multiple types can be respectively continuously produced, and an in vitro high-frequency regeneration system for roots, stems and leaves of polygonatum sibiricum red is established; a high-frequency regeneration system for the roots and stems can also be obtained by adopting the same culture medium. The roots, stems, leaves, callus tissues and rhizomes obtained by utilizing the method can be mutually transformed and grow in different culture stages.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and a culture medium for establishing a high-frequency regeneration system of Taishan Polygonatum by using isolated bud scales of Taishan Polygonatum as explants. Background technique [0002] Taishan Polygonatum ( polygonatum sibiricum Red. ) is a perennial herb of the Liliaceae Polygonatum, also known as tiger ginger, chicken head ginseng, fairy surplus grain, etc. There are about 40 kinds of plants in this genus, which are mainly distributed in the northern temperate zone and the northern subtropical zone. There are 31 species in my country, and they are distributed in most provinces and cities in the country. Taishan Polygonatum, Polygonatum Dianensis and Polygonatum Polyflora are identified as authentic medicinal varieties by the "Chinese Pharmacopoeia" (2010 edition), mainly using their fleshy rhizomes as medicines, with sweet and flat medicinal properties; it belongs...

AI Technical Summary

Problems solved by technology

[0005] Although the above studies on Polygonatum tissue culture have all been successful, they are all limited to the establishment of a rapid propagation system using the underground rhizomes of Polygonatum as explants, and the rhizome explants are easy to carry various pathogens because they are buried deep in the soil for a long time. It is not conducive to thorough disinfection, and it is easy to lead to a higher explant contamination rate and a lower aseptic body formation rate; while using rhizome top buds and shoot tips as explants, although the success rate of aseptic bodies has been improved, there are complications. The source of culture materials is single, the aseptic operation of stem tip stripping is not easy to control, and a large amount of root buds are consumed, which leads to the problem that the rhizomes of Polygonatum rhizomes without terminal buds are difficult to germinate and grow.

Therefore, judging from the current research status of Polygonatum tissue culture, there are still some limitations in the research on the tissue culture technology of Polygonatum, and there are few explorations on the tissue culture technology of different organs of Polygonatum, and there is still a certain technical gap in the tissue culture of Polygonatum.