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Method for fast detecting gamma-aminobutyric acid in baijiu

A technology of aminobutyric acid and white wine, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of liquid phase-derivatization method instability, derivatization reagent poisonous, unfavorable to human health, etc., achieve excellent separation effect, save Effects of Human, Reproducible Satisfaction

Inactive Publication Date: 2017-02-22
ANHUI RUISIWEIER TECH
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Problems solved by technology

Among them, the colorimetric method can only be semi-quantitative, the liquid phase-derivatization method is unstable, time-consuming and troublesome to operate, and the derivatization reagent is toxic, which is not conducive to human health

Method used

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  • Method for fast detecting gamma-aminobutyric acid in baijiu
  • Method for fast detecting gamma-aminobutyric acid in baijiu
  • Method for fast detecting gamma-aminobutyric acid in baijiu

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Embodiment Construction

[0052] For verifying the accuracy of the method of the present invention, get several liquor samples of known gamma-aminobutyric acid content and measure by the method of the present invention, concrete steps are as follows:

[0053] (1) Pretreatment of the liquor sample to be tested:

[0054] Accurately measure 50mL of the liquor sample to be tested in a 100mL rotary evaporator bottle, and evaporate to 3-5mL to remove the alcohols in the liquor to be tested, then dilute to 10mL with ultrapure water, and finally pass through a 0.22μm filter needle filter to obtain the sample to be tested;

[0055] (2) The conditions for setting the ultra-high performance liquid chromatograph are:

[0056] The chromatographic column is ACQUITY UPLC BEH C18 1.7μm 2.1×50mm Column liquid chromatography column;

[0057] The column temperature is 40°C;

[0058] Sample chamber temperature is 20°C

[0059] Mobile phase: A phase is acetonitrile, and B phase is an aqueous solution of acetic acid wit...

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Abstract

The invention discloses a method for fast detecting gamma-aminobutyric acid in baijiu. The method is characterized in that an ultra-high performance liquid chromatography serially connected triple quadrupole mass spectrometer is used for detecting the gamma-aminobutyric acid in baijiu; after the rotary evaporation for alcohol removal on a baijiu sample to be tested, an ACQUITY UPLC BEH C18 1.7 mum 2.1*50mm Column liquid chromatography column is used; an acetonitrile (A)+0.1 percent acetic acid water solution (B) is used as a flowing phase to perform isocratic elution; after a sample is subjected to ultra performance liquid chromatography separation, detection is performed through the triple quadrupole mass spectrometer. The method provided by the invention has the advantages that the method is simple; the qualitative and quantitative detection on gamma-aminobutyric acid in the baijiu can be accurately performed; the scientific basis is provided for the accurate judgment and fast detection of the gamma-aminobutyric acid in the baijiu.

Description

technical field [0001] The invention relates to a method for rapidly detecting γ-aminobutyric acid in liquor, in particular to a method for rapidly detecting γ-aminobutyric acid in liquor by using ultra-high performance liquid chromatography connected in series with a triple quadrupole mass spectrometer, which belongs to wine Analyze technical fields. Background technique [0002] γ-Aminobutyric acid (GABA), as a naturally occurring non-protein amino acid, is an important nutrient in liquor. It is a very important inhibitory neurotransmitter in the central nervous system and has extremely rich physiological functions, such as: (1) sedative nerve and anti-anxiety. (2) lower blood pressure. GABA can act on the vasomotor center of the spinal cord, effectively promote the expansion of blood vessels, and achieve the purpose of lowering blood pressure. According to reports, the effective antihypertensive component of traditional Chinese medicine such as Astragalus is GABA. (3)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/88
CPCG01N30/02G01N30/88
Inventor 王银辉刘国英汤有宏左飞
Owner ANHUI RUISIWEIER TECH
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