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Antibody dispersing agent for homogeneous labeling of immuno gold staining and application thereof

A technology for labeling antibodies and dispersants, which is applied to analytical materials, measuring devices, instruments, etc., can solve the problems of poor consistency of labeling effect and large amount of conjugates, and achieve the effects of improving consistency, improving production efficiency, and reducing costs

Active Publication Date: 2020-03-24
北京纳百生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the current immunocolloidal gold direct labeling method has problems such as poor consistency of labeling effect and large amount of conjugates.

Method used

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  • Antibody dispersing agent for homogeneous labeling of immuno gold staining and application thereof

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Experimental program
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Effect test

Embodiment 1

[0014] This embodiment provides an antibody dispersant for homogeneous labeling of immunocolloidal gold, which is obtained by mixing 30 g of bovine serum albumin, 30 g of polyethylene glycol and 40 g of purified water.

Embodiment 2

[0016] This embodiment provides an antibody dispersant for homogeneous labeling of immunocolloidal gold, which is obtained by mixing 30 g of bovine serum albumin, 30 g of polyvinylpyrrolidone and 40 g of purified water.

Embodiment 3

[0018] This embodiment provides an antibody dispersant for homogeneous labeling of immunocolloidal gold, which is obtained by mixing 30 g of polyvinylpyrrolidone, 30 g of polyethylene glycol and 40 g of purified water.

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Abstract

The invention discloses an antibody dispersing agent for homogeneous labeling of immuno gold staining and application of the antibody dispersing agent, and belongs to the technical field of immuno gold staining. The antibody dispersing agent for homogeneous labeling of immuno gold staining comprises the following components in percentage by mass of 0-30% of bovine serum albumin, 0-30% of polyvinylpyrrolidone, 0-30% of polyethylene glycol and the balance of solvent. The antibody dispersing agent can be applied to an immune colloidal gold homogeneous labeled antibody. According to the technicalscheme, the consistency of the labeling effect can be remarkably improved, and the feeding amount of the to-be-labeled sample is reduced.

Description

technical field [0001] The invention relates to the technical field of immune colloidal gold, in particular to an antibody dispersant for homogeneous labeling of immune colloidal gold and its application. Background technique [0002] Immune colloidal gold technique (Immune colloidal gold technique) is an immune labeling technique that uses colloidal gold as a tracer marker to detect antigens and antibodies. The English abbreviation is: GICT. Colloidal gold is made from chloroauric acid (HAuCl 4 ) under the action of reducing agents such as ascorbic acid, sodium citrate, etc., reductively polymerize into gold nanoparticles of a specific size, and become a stable colloidal state due to electrostatic interaction, called colloidal gold. Colloidal gold particles are negatively charged and can form a strong bond with the positively charged groups of protein molecules. Since this bond is electrostatic, it does not affect the biological characteristics of the protein. In addition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/532
CPCG01N33/532
Inventor 杨春江袁志波马孝斌
Owner 北京纳百生物科技有限公司