O -type foot-and-mouth disease antibody horizontal detection test paper and preparation method

A technology for horizontal detection and foot-and-mouth disease, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of frequent antigen variation, difficult diagnosis, and large antigen difference, and achieve intuitive and easy result judgment, rapid detection process, and reproducible good sex effect

Active Publication Date: 2008-08-06
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

FMDV antigenic mutations are frequent, and the antigenic differences

Method used

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  • O -type foot-and-mouth disease antibody horizontal detection test paper and preparation method
  • O -type foot-and-mouth disease antibody horizontal detection test paper and preparation method

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0035] Specific examples of the invention are provided below.

[0036] (1) Preparation of test strips:

[0037] 1) Prepare the immune gold label layer

[0038] The preparation of i colloidal gold: get 100mg chloroauric acid and be dissolved in 1000ml triple distilled water, add 20ml concentration and be 1% trisodium citrate, boil 15 minutes, obtain the colloidal gold solution of 40 nanometers after cooling;

[0039] ii Colloidal gold-labeled cells to culture foot-and-mouth disease virus, take 100ml of colloidal gold solution, adjust pH to 8.0 with 0.2mol / L potassium carbonate solution, add 0.15mg of O-type foot-and-mouth disease whole virus, stir for 20 minutes, then add 500mg of bovine serum albumin, continue to stir for 15 minutes minutes, and stand at 4°C for 2 hours. The O-type foot-and-mouth disease whole virus used here can be a purified natural virus, or a gene recombinant expression O-type foot-and-mouth disease virus and then purified by differential centrifugation o...

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Abstract

The invention relates to a colloidal gold test paper strip for carrying out the quick detection of the O-shaped foot and mouth disease antibody level of artiodactyls, the preparing method of the test paper strip and a specific detection method. A sample application absorption cushion made of porous fiber material, an immune gold label layer which is arranged on the tail end of the sample application absorption cushion and is connected with the sample application absorption cushion, a detection section made of nitrocellulose film, and an absorption section which is made of water absorption material and is arranged on the tail end of the detection section and is connected with the detection section are fixed on the base plate of the test paper strip. A quality control line is an O-shaped foot and mouth disease resistant rabbit or guineapig IgG antibody, a detection line is coated with O-shaped foot and mouth disease virus, and the O-shaped foot and mouth disease virus of the detection line of the detection section and the colloidal gold label attached to the immune gold label layer is natural virus, or DNA recombination expression foot and mouth disease virus, or the decomposed antigen of the foot and mouth disease.

Description

technical field [0001] The present invention relates to a detection substance for rapid detection, in particular to a test strip for rapid detection of O-type foot-and-mouth disease antibody level in cloven-hoofed animals, preparation of the test strip, and use of the strip to detect A specific detection method for the level of foot-and-mouth disease antibody in artiodactyl animals. Background technique [0002] Foot-and-Mouth disease (FMD) is an acute, febrile, highly contagious animal disease that can spread quickly and over long distances caused by foot-and-mouth disease virus. The targets of infection are pigs, cattle, sheep and other major livestock species, as well as other domestic and wild artiodactyls, with more than 70 susceptible species. The incidence rate of foot-and-mouth disease is 100%, and the outbreak can bankrupt farms, and the animal husbandry and even the entire national economy in the affected area and country suffer a huge blow. Therefore, the World ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/52
Inventor 蒋韬梁仲王超英杨亚民刘西兰陈涓张军翟国元才学鹏卫广森刘湘涛刘在新任维维智晓莹祁光宇
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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